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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for 7 Chloro 1 iodonaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed at 4 days in vitro (DIV4) with 4% PFA and the Duolink PLA kit was employed as previously described 7 following the manufacturer’s (Sigma-Aldrich) protocol 45.
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bioRxiv - Plant Biology 2023Quote: ... Flag-ELF7 and GFP-VIP3 ChIP-seqs were performed using 7-day-old seedlings grown under standard conditions and an anti-Flag M2 antibody (F1804, Sigma) and an anti-GFP antibody (ab290 ...
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bioRxiv - Cell Biology 2023Quote: ... incubating them in 0.5 ml cFAD with 3.75×10^7 PFU/ml supplemented with 4 μg/ml Polybrene (Sigma Aldrich, H9268) on top of the insert and 2 ml cFAD on the bottom ...
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bioRxiv - Cancer Biology 2023Quote: ... T47D and MCF-7 cells were incubated in phenol red-free charcoal-stripped medium with E2 (10 nM; Sigma-Aldrich) for 5 min and 15 min/37°C ...
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bioRxiv - Immunology 2023Quote: Thymic lobes were isolated from 1- to 3-day old neonatal WT or RIP-mOVA mice and cultured for 7-10 days on a filter-strainer stack (filter: MF-Millipore Membrane Filter ...
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bioRxiv - Plant Biology 2023Quote: ... The 30 collected fractions were combined into 15 pools and desalted on C18 Cartridges (Empore SPE Cartridges C18 standard density, bed I.D. 7 mm, volume 3 ml, Sigma-Aldrich). Each fraction was concentrated by vacuum centrifugation and reconstituted in 40 µl of 0.1% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer-exchanged against HBS-E (10 mM HEPES at pH 7, 150 mM NaCl, 2 mM EDTA) by ultrafiltration using Amicon Ultra devices (Millipore).
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bioRxiv - Developmental Biology 2023Quote: ... 20-40 µg of total protein was separated on 7-10% SDS-PAGE polyacrylamide gels and transferred onto Immobilon PVDF membranes (Millipore). Membranes were blocked using 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Mettl3flox/flox and Mettl3VilCreERΔ/Δ mice were moved to new cages at 7 weeks of age and given sterile DI water supplemented with 0.5g/L Ampicillin (Sigma A9518), 0.5g/L Neomycin (MP Biomedicals 180610) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 U/ml of Penicillin-Streptomycin, and freshly added TGF1 (10 ng/ml, 7754-BH, RD Systems) and 10−7 M dexamethasone (Dex, D8893, Sigma). Osteogenic differentiation medium ...
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bioRxiv - Immunology 2023Quote: ... and then digested in 7 ml Hank’s balanced salt solution (HBSS) with 0.5% bovine serum albumin (Sigma-Aldrich, Cat# A2153), 2% FCS ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-PMCA4bLA and PMCA4-specific shRNA expressing MCF-7 lines were mixed with cold Matrigel (Corning Matrigel, DLW356231, Sigma-Aldrich) and 15 µl of cell-Matrigel mixtures were added to removable 8-well chambers (80841 ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, Sigma Aldrich, St Louis, MO, USA). This solution was applied to the autaptic culture neurons for 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Bioengineering 2023Quote: ... 7 μL of the cells and fibrinogen solution was combined with 7 μL of VascuLife with 2.8 U/mL thrombin from bovine plasma (Millipore Sigma) prior to injection in the device gel port (for a final concentration of 7 million ml-1 HUVECs ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Non-anesthetized 7 dpf Tg(HuC:H2B-GCaMP6F; casper) larvae were immobilized at in 2% low melting point agarose (Sigma-Aldrich) prepared in Danieau solution and mounted dorsal side up in a custom-made perspex cylindrical Danieau-filled chamber ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and a 7-point calibration curve from 125–10 µg/mL were determined using an enterobactin standard (Sigma Aldrich #E3910). To minimize carryover the needle was washed for 10 sec before and after each injection ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then treated with 16 μM of the ROS-sensitive fluorogenic probe 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA, Sigma-Aldrich) for 1 h in the dark at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... CAS#96736-11-7) was used at a final concentration of 100 μg/ml and thiabendazole (TBZ) (Sigma-Aldrich; T8904) at 5-15 μg/ml in YE media where appropriate ...
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bioRxiv - Biophysics 2021Quote: ... two solutions of 500 mM ATP and two solutions of 500 mM ADP were made in 50 mM HEPES (pH 7; Sigma-Aldrich). The pH was measured using a Thermo Scientific Orion Star pH meter ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... 20 µg of proteins in each sample were precipitated by methanol/chloroform method and re-suspended in 30 µl of multichaotropic sample solution UTT buffer (7 M Urea, 2M thiourea, 100 mM TEAB [Sigma-Aldrich]) and 10 µl of 20% SDS was added ...
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bioRxiv - Microbiology 2022Quote: ... and sample inputs were normalized to identical OD260 value in 300-400 μL total volume which was layered onto a 7-47% (w/v) linear sucrose gradient (Sigma BioUltra) in bacterial polysome buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... Fe(III) glutamic acid (5 mM FeCl3 + 50 mM glutamic acid, pH 7, bought from Sigma, CAS number: 56-86-0) and Fe(III ...
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bioRxiv - Biochemistry 2019Quote: ... After 6 or 7 days cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with 100 μL of PBS and incubated with 100 μL of 20 μM 2’,7’-dichlorofluorescin diacetate (DCFH-DA, Sigma D6883) in DMEM in the dark for 40 min at 37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... CYP1A2 and CYP2A6 activities were measured by incubating the cultures with 5 μM 7-ethoxyresorufin or 50 μM coumarin (Sigma-Aldrich) for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 1.7×103 g) pellet was resuspended in 900 μl of ice-cold 0.1% NP40 (IGEPAL® CA-630, I8896, Sigma Aldrich) in PBS and 300 μl of the lysate (whole cell lysate fraction ...
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bioRxiv - Synthetic Biology 2021Quote: ... a freshly prepared solution of 5-(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; Sigma-Aldrich Co., St. Louis, MO, USA) in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in the serum were removed by passing it through a pre-rinsed (7 times washed) Amicon Ultra-2ml 3000 MWCO (Merck Millipore, USA) column ...
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bioRxiv - Immunology 2020Quote: ... Cells were centrifuged at 300 g for 7 min and erythrocytes were lysed for 10 min with Red Blood Cells Lysis Buffer (Sigma-Aldrich). After a second centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Unisexual and bisexual mating assays were induced on either 5% V8 juice agar medium (pH = 7) or Murashige and Skoog (MS) medium minus sucrose (Sigma-Aldrich) in the dark at room temperature for the designated time period.
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bioRxiv - Neuroscience 2020Quote: ... hyposmia was induced by bilateral intranasal injections with 20 μL of 5% (0.17M) zinc sulfate (Sigma-Aldrich, catalog #7446-19-7) in male C57BL/6N mice ...
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bioRxiv - Microbiology 2021Quote: ... The infected host cells were incubated for 7 min at 37°C with DMEM containing 3 μM of calcium ionophore A23187 (C7522, Sigma-Aldrich) prior to fixation with 4% PFA ...
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bioRxiv - Microbiology 2020Quote: VeroE6 cells expressing TMPRSS2 (VeroE6/TMPRSS2)7 were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: α-synuclein aggregates in the head muscles of 7-days old worms were visualized by fluorescence microscopy (400x magnification) in worms anesthetized with 15 mM sodium azide (Sigma, S2002). Pictures were segmented using Ilastik (version 1.3.0 ...
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bioRxiv - Plant Biology 2022Quote: Proteasome activity was measured using the fluorogenic substrate N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin (Millipore Sigma; S6510) as described Üstün et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... bacterial densities were determined by plating out 100 µl of a dilution series ranging from 10−5 to 10−7 on Vibrio selective Thiosulfate Citrate Bile Sucrose Agar (TCBS) plates (Sigma Aldrich). Plates were incubated over night at 25° C and the total amount of colonies was counted the following day.
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 105 IL-7 dependent primary Irf4−/− preB-I cell cultures were resuspended in 400 µL RPMI medium (D5030, Sigma-Aldrich) with 600 µL viral supernatant and 1.5 µL polybrene and spun in culture plates at 2700 RPM for 90 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... until 10-7 and 10 µl of the dilutions between 10-5 and 10-7 were plated in BHI agar plates supplemented with Congo Red (Sigma®) at a final concentration of 25 µg.ml-1 ...
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bioRxiv - Microbiology 2022Quote: ... and 100 μl spread on either LB agar plates with or without specified antibiotics (2 μg/ml amikacin disulfate salt or 7 μg/ml nitrofurantoin (both Sigma-Aldrich) or 50% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... bone marrow cells were flushed from dissected femurs and tibias and cultured for 7 days in macrophage growth medium (RPMI with 10% heat-inactivated FBS (Sigma-Aldrich), 10% 3T3 m-CSF ...
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bioRxiv - Microbiology 2022Quote: ... For verification of the expected product size and RNA integrity, RNA gels (6 % PAA, 7 M urea) were prepared with subsequent staining using StainsAll (Sigma-Aldrich).
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bioRxiv - Bioengineering 2022Quote: ... The retinal strips were sectioned (7–10 µm thick) and placed on microscope slides that were treated with 3-Aminopropyltriethoxysilane (APES, Sigma A3648). Before immunohistochemistry ...
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bioRxiv - Plant Biology 2021Quote: ... Lipids extracts were incubated 1 h at 50°C in 2 mL of methylamine solution (7 ml methylamine 33% (w/v) in EtOH combined with 3 mL of methylamine 40% (w/v) in water (Sigma Aldrich) in order to hydrolyze phospholipids ...
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bioRxiv - Plant Biology 2021Quote: The glass fibre filter papers (GFFPs) used to collect each sample were submerged in 7-mL volumes of HPLC-grade methanol (>99.9%, Millipore-Sigma Canada) in 20-mL scintillation vials (Chromatographic Specialties Inc. ...
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bioRxiv - Microbiology 2020Quote: ... CD14+ macrophages differentiated for 7 days in M-CSF (described above) were detached from the petri dish using 2 mM ethylenediaminetetraacetic acid (EDTA, Sigma Aldrich) in PBS at 4 °C as well as mechanical scraping ...