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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for 2 Chloro 3 4 cyanobenzoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) for 5 min at RT in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... 4 kDa FITC-dextran and 2 MDa FITC-Dextran were from Sigma-Aldrich. 70 kDa TRITC-dextran was from Chondrex Inc ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... 3 × 4 ml culture sample were applied in parallel onto three 0.45 μm nitrocellulose filters (Millipore) connected to vacuum ...
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bioRxiv - Cell Biology 2021Quote: ... and blocked for at least an hour at 4° in PBST + 3% normal goat serum (Sigma). Tissues were incubated overnight at 4° with gentle agitation with primary antibodies diluted in PBST and washed three times with PBST ...
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bioRxiv - Microbiology 2023Quote: L-Sulforaphane (4-methyl sulfinyl butyl isothiocyanate, 4MSOB-ITC; ≥ 95 %, CAS 142825-10-3, Sigma-Aldrich) or allyl-ITC (allyl isothiocyanate ...
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bioRxiv - Neuroscience 2023Quote: ... 3 colonies of each construct were selected and incubated in 4 mL LB medium (Sigma #L3022) with 1:1000 carbenicillin overnight in a shaker at 37°C and 250 RPM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assays were stopped by filtration at 14,000g and 4 °C with 3 kDa spin filters (Millipore). After filtration ...
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bioRxiv - Neuroscience 2023Quote: ... for another 3 days of cultivation before fixing with 4 % paraformaldehyde (PFA, #P6148, Sigma-Aldrich, USA) for 30 min at 37 °C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Cells were washed 3 times with PBS and then fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... followed by tenfold concentration using Amicon Ultra-4 Centrifugal Filter (3 kDa MWCO, Millipore Sigma UFC800396). Custom-designed 4-Plex Luminex kits (R&D Systems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Odor stimuli were diluted to 10−3 and 10−4 concentration in hexane (>99.5% purity; Sigma), with hexane used as the solvent control ...
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bioRxiv - Developmental Biology 2024Quote: ... 4× SDS sample buffer (0.225 M Tris-HCl pH6.8, 50% glycerol (Sigma-Aldrich, 12-1120-3), 5% SDS ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Biophysics 2020Quote: ... pooled and concentrated to 2-4 mg/mL using Amicon Ultra-4 (100 kDa molecular weight cut off) concentrator tubes (EMD Millipore), flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biophysics 2020Quote: ... MEFs transfected with LIM-protein plasmids were plated in 4-well Strex chambers (STB-CH-4W, 2 cm2 × 4 chambers) coated with 50 μg/mL fibronectin (Millipore, FC010), and allowed to recover overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at room temperature or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma] ...
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bioRxiv - Cell Biology 2021Quote: ... XL-413) for 1 h followed by inhibitor incubation together with 100 μM 5-chloro-2ʹ-deoxyuridine (CldU; Sigma-Aldrich, Germany) and ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759, 40 mM sodium phosphate, Sigma S5011 ...
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bioRxiv - Microbiology 2021Quote: ... Three subsamples (∼2 ml) were taken from each section with a 3 ml syringe and immediately fixed in 2 ml RNAlater (Sigma Life Science) in a 5 ml tube (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt, Aldrich) or negatively charged 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, Sigma-Aldrich, Inc) containing N,N’-methylenebisacrylamide (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... The dried extracts were dissolved in 15 μL or 20 μL of methoxyamine hydrochloride solution (40 mg/mL in pyridine, both Sigma Aldrich, St. Louis, Missouri, U) and incubated for 90 min at 30 °C with constant shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 μl of 5mg/ml MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: Spheroids were cultured on dishes coated with 3% poly-2-hydroxyethyl methacrylate (polyHEMA) (Sigma, P3932), which was dissolved in 95% absolute ethanol overnight in magnetic stirrer at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... RPE1 cells and LCLs were treated with 3 mM or 2 mM thymidine (Sigma, T9250) for 22 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Media was changed every 2-3 days for new media: DMEM/F-12 (Sigma-Aldrich) + 10% FBS (Gibco ...
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bioRxiv - Genomics 2020Quote: ... 3·106 cells were fixed in 400 μl of DPBS/2% Paraformaldehyde (Sigma P-6148) for 10 minutes at room temperature shaking ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) containing protease inhibitor cocktail and phosphatase inhibitor cocktails 2 and 3 (Millipore-Sigma). 15 adult Drosophila males and females of different genotypes were harvested at 10 days post-enclosure ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) containing protease inhibitor cocktail and phosphatase inhibitor cocktails 2 and 3 (Millipore-Sigma). 15 adult Drosophila males and females of different genotypes were harvested at 10 days post-enclosure ...
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bioRxiv - Neuroscience 2020Quote: ... coated tissue culture dishes and routinely sub-cultured every 2-3 days using accutase (Sigma) to detach them.
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bioRxiv - Neuroscience 2020Quote: ... and phosphatase inhibitor cocktail 2 and 3 (sc-24948 Sigma-Aldrich, St. Louis, MO, USA). Total Aβ42 was measured via ELISA (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 mM NaCl and 3 parts of 1x PBS and 2% methanol-free formaldehyde (Sigma) for 15 min at RT on an overhead rotator ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... and 3 ug/ml 5-fluoro-2’-deoxyuridine + 7 ug/ml uridine (FRD+U; Sigma) added ...
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bioRxiv - Microbiology 2021Quote: ... 6×104 HPMEC or 2×105 Calu-3 cells were seeded on 0.2% gelatin-(Sigma) coated glass coverslips in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... only Group-3 animals were treated with 2 mg/kg Dexamethasone sodium phosphate (Sigma Aldrich) in PBS by subcutaneous injection three times for one week ...
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bioRxiv - Immunology 2020Quote: ... only Group-3 animals were treated with 2 mg/kg Dexamethasone sodium phosphate (Sigma Aldrich) in PBS by subcutaneous injection three times ...
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bioRxiv - Physiology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay from Sigma (St. Louis, MO), and D-luciferin from Gold Biotechnology (St ...
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bioRxiv - Developmental Biology 2020Quote: ... 2-3 fillets of late foraging larvae were incubated in M3 insect medium (Sigma-Aldrich) supplemented with 2 mM of trehalose (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-mercaptoethanol) containing 10 µM SB431542 (R&D) and 3 µM CHIR99021 (Sigma), and was subsequently replaced every day ...
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bioRxiv - Cell Biology 2021Quote: ... coated tissue culture dishes and routinely sub-cultured every 2–3 days using accutase (Sigma) for detachment ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...