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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... for 2 hours at room temperature followed by administration of primary antibodies such as ChAT (goat polyclonal, 1:500, EMD Millipore AB144P), Pax2 (rabbit polyclonal ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were incubated with 50μL primary antibody solution (dilution 1:1000) in 2% BSA (Bovine Serum Albumin, Sigma Cat. No.: A7906) at 4°C cold room overnight ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542) at RT for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and MRS 1220 (N-(9-chloro-2-furan-2-yl-[1,2,4]triazolo[1,5-c]quinazolin-5-yl)-2-phenylacetamide) were purchased from Sigma-Aldrich and dissolved in dimethyl-sulphoxide (DMSO) ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) photoinitiator (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2019Quote: RNA was extracted by RNA miniprep kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated using GenElute RNA Miniprep Kit (Sigma) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... MultiScreen® HTS 96 multiwell plates (pore size 0.45 μm) with high protein-binding membrane (hydrophobic Immobilon-P PVDF membrane) were purchased from Millipore (Amsterdam, The Netherlands), conical 96-well Nunc plates from Thermo ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed again 3 times for 5 minutes each in PBS then non-specific binding sites were blocked in 1% bovine serum albumin (Sigma-Aldrich, A9418) in PBS for approximately 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... For RBD inoculations eight-week-old male BALB/c mice were immunized with 7.5 μg of the receptor binding domain of Spike protein (kindly gifted by Andrea Gamarnik, Argentina) in 75 μL Complete Freund’s Adjuvant (CFA, Sigma, St. Louis, MO) via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA ...
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bioRxiv - Cancer Biology 2021Quote: ... and calmodulin-binding peptide (CBP) was affinity-purified by using streptavidin-conjugated agarose beads (EZview™ Red Streptavidin Affinity Gel, Sigma-Aldrich). The initial protein peaks containing EP400NL were collected and pooled together after the size exclusion chromatography and incubated with streptavidin affinity beads at 4 °C overnight with constant rotation ...
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bioRxiv - Bioengineering 2021Quote: ... A 25-µL aliquot of fluorescently labeled aptamer probe in binding buffer (wash buffer containing 1% sheared salmon sperm DNA and 300 µM dextran sulfate sodium salt (40 kDa, Sigma-Aldrich, 42867)) was added to each well and allowed to incubate for 60 minutes while shaking at 500 RPM ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Molecular Biology 2019Quote: The nucleic acid binding property of rcCBP2 was further validated using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 3µg of rcCBP2 was mixed with 500 µl cellulose matrix [1 mg/ml matrix prepared in 50 mM sodium phosphate (pH 7.6) ...
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bioRxiv - Microbiology 2020Quote: ... aureus binding to fibronectin and fibrinogen was determined using a semi-quantitative adherence assay on 96-well tissue culture plates (Sigma-Aldrich, USA). Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6 ...
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bioRxiv - Neuroscience 2022Quote: ... Permeabilization of the membrane and blocking of unspecific binding sites were performed in one step by adding 0.5% Triton X-100 (Sigma-Aldrich, Buchs, Switzerland) to the blocking solution (PBS - Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: The nucleic acid binding property of protein was further validated using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 μgm recombinant protein was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: Gus activity was determined to quantify chitin binding of Gus-Cts1 using the specific substrate 4-methylumbelliferyl β-D galactopyranoside (MUG, Sigma–Aldrich). To his end 50 μg of U ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 40 μl of Protein A/G magnetic beads was incubated with 5 μg anti-pan-ADP-ribose binding reagent (Millipore Sigma, MABE1016) per IP sample in 100 μl PBS for 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted using an RNA Isolation Kit (Sigma-Aldrich Spectrum™ Plant Total RNA) according to suppliers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Neuroscience 2020Quote: ... in 5 mL of 2-methyl-2-butanol (Sigma Aldrich, #240486). Mice were deeply anesthetized using 15 µL of Avertin 2.5% per gram of body weight ...
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bioRxiv - Bioengineering 2021Quote: ... (2) hexafluorophosphate azabenzotriazole tetramethyl uronium (HATU; 2 equiv:ELP amine, Sigma, 445460), and (3 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM penicillin/streptomycin and 2 mM L-glutamine (Sigma, UK). Hek-293T cells were plated in 24 well plates for 24 hours before transduction with LV-LTα or LV-GFP at MOI 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μM ponatinib (Selleckchem) or 2 μM iodoacetamide (IAA) (Sigma-Aldrich), the other half was treated with vehicle (DMSO or water ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg/mL 4′,6-Diamidin-2-phenylindol (DAPI, Sigma-Aldrich) in 1X PBS was added for 2 min followed by a final washing step ...
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bioRxiv - Microbiology 2020Quote: ... 2% glycerol or 2% casamino acids and in RPMI-1640 (Sigma, containing L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... E2 and P4 were solubilized in 2-butanol (2-BtOH; Sigma) as our previous studies demonstrate that this reagent does not interfere with NgPLD activity ...
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bioRxiv - Biochemistry 2019Quote: 2 µl of 1 mM 2’,7’ Dichlorofluorescein diacetate (Sigma-Aldrich) dissolved in DMSO was added to 198 µl of cell culture in a black f-bottom 96 well plate (Greiner bio-one ...
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bioRxiv - Immunology 2019Quote: ... and recombinant human interleukin-2 (rhIL-2; 25U/ml; Sigma-Aldrich). To mimic a transient stimulation ...
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bioRxiv - Biochemistry 2019Quote: ... Released glycans were labelled with 2-aminobenzamide (2-AB; Sigma-Aldrich) and purified on Whatman 3 mm chromatography paper ...
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bioRxiv - Bioengineering 2020Quote: ... The photoinitiator (2-hydroxy-2-methylpropiophenone, Darocur 1173, 405655, Sigma-Aldrich) is introduced with the two precursors.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25mM Hepes (N-[2-hydroxyethyl]piperazine-N0-[2-ethanesulfonic acid; Sigma]), supplemented with 10% heat inactivated FBS (GIBCO ...
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bioRxiv - Molecular Biology 2019Quote: ... or MG132 (10 μM, 2 h, replicates 2, 3, 4; Sigma). Proteins were precipitated in acetone ...
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bioRxiv - Immunology 2020Quote: 2-deoxy-d-glucose (2-DG; Sigma-Aldrich, St. Louis, MO) treatment of wounded zebrafish larvae (simple tail transection ...
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bioRxiv - Neuroscience 2022Quote: 2-Bromopalmitate (or 2-bromohexadecanoic acid, Sigma-Aldrich, Cat no. 238422) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... 2% pectone (Difco) and 2% glucose (Sigma-Aldrich, Carlsbad, CA, USA) or selective medium YNB containing ...
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bioRxiv - Synthetic Biology 2022Quote: ... was mixed with photo-initiator 2-hydroxy-2-methylpropio-phenone (Sigma) (90/10% w/w) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg/ml doxycycline and 2 µM Ara-C (Sigma-Aldrich). From day 3 – 6 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mm samples were embedded instead in 2% agarose (Sigma-Aldrich) in 0.15 M CaC or in water (depending on the last staining step that the sample was exposed to ...
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bioRxiv - Immunology 2022Quote: ... followed by clearing/mounting using 2’-2’-thio-diethanol (Sigma, 166782) as previously described6 ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM potassium hexacyanoferrate(II) trihydrate (C6FeK3N6+2 · 3H2O; Sigma Aldrich), 2 mM potassium hexacyanoferrate(III ...