Labshake search
Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Recombinant Mouse Timd4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... The hexahistidine-tagged protein was expressed in Hi5 insect cells (Trichoplusia ni) grown in 1 liter of M3 medium (Sigma) supplemented with 10% fetal bovine serum (Wisent) ...
-
bioRxiv - Immunology 2024Quote: ... cells expressing GFP-tagged CD80 (Omar S Qureshi et al., 2011) in CHO cell medium (DMEM containing 25 mM D-glucose (Sigma), 1 mM sodium pyruvate (PAA) ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
-
bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... or full length DDX50) and 50 ng N-terminally tagged HaloTag-GID4 using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: ... Streptactin resin was washed with 40 column volumes (CV) wash buffer and SBP-tagged protein was eluted using wash buffer supplemented with 10 mM desthiobiotin (Sigma). Eluate was concentrated to 500 µl using a 100 kDa MWCO concentrator (amicon ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the soluble fractions containing the FLAG-tagged KDM2A protein were affinity purified using the anti-FLAG M2 affinity gel (Sigma). Gel beads were washed with TBS buffer (50 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 30kDa MWCO concentrator (EMD Millipore) to 0.2mL ...
-
bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
-
bioRxiv - Genetics 2024Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
-
bioRxiv - Cell Biology 2024Quote: ... HA-tagged proteins were probed with a 1:1000 dilution of Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
-
bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
-
bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
-
bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
-
bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
-
bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
-
bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
-
bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
-
bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
-
bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
-
bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
-
bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
-
bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
-
bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
-
bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
-
bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
-
bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
-
bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml) and 2i (1μM PD325 ...
-
bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
-
bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...
-
bioRxiv - Neuroscience 2021Quote: ... the astrocytes were stimulated with TNF recombinant (10 ng /ml) (Sigma-Aldrich Corporation, Missouri, EUA) or MCM (300 µl ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
-
bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
-
bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...
-
bioRxiv - Microbiology 2019Quote: Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was purchased from Sigma. The protease inhibitor dec-RVKR-CMK was obtained from Tocris ...
-
bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...