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Citations for Millipore Sigma :
1051 - 1100 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and supplemented with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore). Cultured mESCs at 60% confluency were washed with PBS twice before the preparation of total lysate ...
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bioRxiv - Developmental Biology 2022Quote: ... FGF treatments were done using recombinant Fibroblast Growth Factor-Basic (F3685, Sigma-Aldrich) concentrations were 300 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA (10 µg) was incubated with recombinant RNAse-free DNase I (Sigma) in 50 µl-reaction tubes at 37° C for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were DNase I digested (DNase I recombinant, Rnase-free, Millipore Sigma), cleaned up (RNA Clean & Concentrator-5 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated with recombinant Cholera toxin B (CTxB)-FITC (Sigma Aldrich C1655) for 1 h to stain for lipid rafts and then with an anti-TLR4 antibody (Abcam ab22048 ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant plasmids were transformed into BL21 (DE3) or BL21 (DE3) Tuner cells (Novagen) and were incubated in 1L LB in 2L conical flasks at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Neuroscience 2023Quote: ... Michigan) and 0.6 TIU/mL of recombinant aprotinin was added (Sigma; Burlington, MA). For saliva ...
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biochemistry 2023Quote: ... periplasmic expression of the recombinant GFP-nanobody in BL21(DE3) Rosetta cells (Novagen) was induced with 0.1 mM IPTG for 16 hours at 16°C ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... into PBS with 0.05% BSA and 5 µL/mL recombinant RNAse inhibitor (Sigma). Isolation of single cells was verified with a haemocytometer ...
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bioRxiv - Bioengineering 2024Quote: ... Cell culture medium was supplemented with 1.5 μg mL−1 recombinant ApoE (Sigma), prepared according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107) (Day −1).
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bioRxiv - Immunology 2024Quote: ... Recombinant FasL-LZ was purified using magnetic beads conjugated to anti-FLAG (Sigma). Quantification of recombinant protein was determined by ELISA (R&D) ...
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bioRxiv - Evolutionary Biology 2024Quote: Recombinant HK3 proteins were expressed in Escherichia coli Rosetta (DE3) T1 (Novagen N59205). Cultures were grown in auto-induction medium 139 at 37°C for 2 hours ...
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bioRxiv - Genomics 2024Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma) at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2024Quote: Chemicals and recombinant proteins were obtained as follows: H-1152 (555550; Sigma Aldrich); Y27632 (08945-84 ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... Degradation of AID-tagged CHD4 was induced by the addition of 500 μM 3-Indoleacetic acid (I2886, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... The GST-tagged proteins were eluted by 10mM reduced glutathione (Sigma, 100mM stock made in 50mM Tris, pH7.6) in PBS (containing 1mM PMSF and 0.02% (∼3mM ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Cell Biology 2023Quote: 75 μg GST-tagged Rab GTPase was loaded with either 10 mM GDP or GTP (Sigma Aldrich, Germany) in the presence of 20 mM EDTA and 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Biochemistry 2024Quote: ... and a N-terminally 10xHis-3xFLAG-TEV tagged MIDEAS were co-transfected into HEK293F cells with PEI (Sigma). After 48 hours of growth ...
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bioRxiv - Biochemistry 2024Quote: The purified MBP-TEV-tagged proteins were concentrated to about 80 µM with a centrifugal filter unit (Millipore) before adding 500 units of TEV protease (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... human insulin was purchased by SIGMA and was used at a final concentration of 7ug/ml ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with human albumin (Sigma, #A0237), sodium DL-lactate (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human serum albumin (Sigma, Catalog # A9511) and human pituitary FSH (National Hormone and Pituitary Program ...
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bioRxiv - Immunology 2021Quote: ... purified human C1q (10μg/ml; Millipore) was added as a source of complement for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 0.5% human serum albumin (Sigma Aldrich). Cell number in the suspension was adjusted to achieve a λ (mean number of cells per droplet ...
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bioRxiv - Cell Biology 2020Quote: ... TANGO1 (rabbit anti–human; Sigma-Aldrich), sec16A (rabbit anti-human ...
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bioRxiv - Cell Biology 2020Quote: ... sec16A (rabbit anti-human; Sigma-Aldrich), calreticulin (goat anti–human ...
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bioRxiv - Biochemistry 2020Quote: ... (iv) 10 nM human insulin (Sigma), and (v ...