Labshake search
Citations for Millipore Sigma :
1051 - 1100 of 6718 citations for MXRA8 protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Zoology 2019Quote: ... Hsp70 protein was labelled with anti-HSP70 (Sigma-Aldrich, # H9776) at 1:3000 ...
-
bioRxiv - Immunology 2019Quote: ... and the antibody was purified with Protein G (Sigma-Aldrich). Mice were injected every 2 days for a total of 9 injections with 1.5 μg of the anti-c-Fms antibody in 3 μL of phosphate-buffered saline (PBS) ...
-
bioRxiv - Physiology 2019Quote: ... followed by determining protein concentration using BCA assay kit (SIGMA). Samples were loaded onto SDS-PAGE for protein expression analysis.
-
bioRxiv - Molecular Biology 2020Quote: 20 μg protein was incubated in 5x Laemmli buffer (Sigma) at 95°C for 10 min and separated by a Mini-PROTEAN® TGX (Bio-Rad ...
-
bioRxiv - Cell Biology 2019Quote: ... The proteins were transferred to PVDF membranes (Immobilon-P, Millipore) and probed with antibodies to detect the FLAG fusions and endogenous proteins ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein concentrations were determined using the Bradford assay (Sigma-Aldrich) with bovine serum albumin as standard.
-
bioRxiv - Systems Biology 2019Quote: ... Separated protein lysates were transferred to PVDF membranes (Millipore #IPVH00010), which were subsequently rinsed in TBST (Tris-buffered saline ...
-
bioRxiv - Genetics 2019Quote: ... Proteins were transferred from gels to Immobilon-P membrane (Millipore) and probed with antibodies as described 20 ...
-
The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... and protein was quantified using a Bradford assay kit (Sigma) following manufacturers’ instructions.
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were detected with antibodies against GFP (Sigma, 1:1000) and FMR polyclonal antibodies (1:1000) ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using the Direct Detect assay (Millipore). Protein samples were run on 4–15% Mini-PROTEAN TGX gels (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: Proteins were extracted using RIPA buffer (Sigma-Aldrich, 20-188) supplemented with Complete Mini EDTA-free Protease inhibitor cocktail (Roche ...
-
bioRxiv - Biochemistry 2019Quote: ... and supplemented with SyproOrange Protein Gel Stain 1:500 (Sigma). After incubation on ice for 1.5 h ...
-
bioRxiv - Biochemistry 2019Quote: ... The transferred proteins were visualized with chemiluminescence detection kit (Millipore).
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were transferred onto PVDF membranes (Millipore, Burlington, MA, USA). The blots were blocked for 2 h at room temperature with 5% non-fat milk in PBS/0.05% Tween 20 and then incubated with the primary antibody (1 µg/mL ...
-
bioRxiv - Physiology 2019Quote: ... Protein transfer was assessed by ponceau red staining (Sigma-Aldrich). Membranes were blocked with 5% bovine serum albumin (BSA ...
-
bioRxiv - Physiology 2019Quote: ... Eluted proteins were run through Amicon Ultracel 10K (Millipore, #UFC501096) to concentrate in a final volume of 50μL ...
-
bioRxiv - Plant Biology 2021Quote: ... and protein was precipitated by 10% TCA (trichloroacetic acid, Sigma) in acetone ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Protein concentration was determined using Bradford reagent (B6916, Sigma-Aldrich). For offline high pH reversed-phase (hpH RP ...
-
bioRxiv - Biophysics 2021Quote: ... Purified protein was concentrated using Amicon Ultra-Centrifugal units (Millipore) and flash frozen for storage at −80°C or used immediately for experiments.
-
bioRxiv - Immunology 2021Quote: ... The proteins were concentrated with Amicon (cutoff 10 kDa, Sigma) to achieve around 0.200 mg/mL of protein solution for mass spectrometry.
-
bioRxiv - Microbiology 2021Quote: ... the protein solution was reduced with 10 mM DTT (Sigma) for 1 h at 37 °C and alkylated with 20 mM IAA(Sigma ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... proteins were transferred to Immobile FL PVDF membrane (EMD Millipore) and immunoblotting was performed with anti-FLAG M2 (F1804 ...
-
bioRxiv - Cancer Biology 2021Quote: ... the proteins were visualized with Chemiluminescent HRP Substrate (Merk Millipore) and signals recorded using Bio-Rad ChemiDoc MP chemiluminescence imaging system (BIORAD).
-
bioRxiv - Cell Biology 2021Quote: ... The proteins were concentrated with Amicon Ultra-15 filters (Millipore) with molecular weight cutoffs of 30 kDa and 50 kDa for ICR1-His6 and ICR2-His6 ...
-
bioRxiv - Biochemistry 2021Quote: ... the proteins were incubated with SYPRO orange dye (Sigma-Aldrich), which binds specifically to hydrophobic amino acids leading to an increased fluorescence at 610 nm when excited with a wavelength of 490 nm ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein A/G agarose beads (EMD Millipore, cat. no. IP05) were incubated with primary antibody for 2 hours at 4°C before addition to cell lysate ...
-
A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... The recombinant protein was produced in Rosetta (DE3) cells (Novagen) and purified by affinity chromatography using a GSTrapFF column ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The total proteins were transferred to PVDF membrane (Millipore, USA). Transferred protein quantities were detected with Ponseau S solution (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2021Quote: ... Eluted protein was crosslinked with 0.04% glutaraldehyde (EM-grade, Sigma) on ice for 25 min ...
-
bioRxiv - Biophysics 2021Quote: SSB protein from Escherichia coli was purchased from Sigma-Aldrich/Merck ...
-
bioRxiv - Cell Biology 2021Quote: ... carrier protein and 0.2 mL of 100% Trichloroacetic acid (Sigma) was added to the extracted protein fraction ...
-
bioRxiv - Cell Biology 2021Quote: ... and protein concentration was determined with Bradford reagent (Sigma-Aldrich). For further analysis ...
-
bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with Protein A Agarose beads (Sigma-Aldrich) for 1 h at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein were transferred to Immobilon-P PVDF membrane (IPVH00010, Millipore) either for 1 hour at 100V at 4°C or overnight at 35V and 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Gli2-ZF protein was expressed in BL21 (DE3) Rosetta (Millipore) E ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were then transferred to Immobilon-FL PVDF membrane (Millipore), blocked in 5% milk in TBST (Tris-buffered saline + 0.1% Tween-20) ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were revealed using chemiluminescent detection (Immobilon Western, Millipore WBKLS0100) and visualized using a Kodak GEL Logic 1500 Imaging System with Transilluminator.
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins were blotted onto Immobilon-P PVDF Membranes (Millipore, USA). For washing of membranes TBS-T (137 mM NaCl ...
-
bioRxiv - Cancer Biology 2019Quote: ... Proteins were fluorescently labelled with Atto 488 NHS ester (Sigma) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA was double-crosslinked to proteins with 37% formaldehyde (Sigma) at a final concentration of 1% ...
-
bioRxiv - Biochemistry 2020Quote: ... the proteins were incubated with SYPRO orange dye (Sigma-Aldrich), which binds specifically to hydrophobic amino acids leading to an increased fluorescence at 610 nm when excited with a wavelength of 490 nm ...
-
bioRxiv - Biochemistry 2020Quote: Proteins were extracted for Western blotting using trichloroacetic acid (Sigma) and glass beads as described in (Cox et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... Solubilized proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich), pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein expression was induced in BL21(DE3) cells (Sigma 69450) at 20°C using 1 mM isopropyl β-D-1-thiogalactopyranoside (dioxane free ...
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were eluted using a 3xFLAG peptide (Sigma #F4799). Samples were then heated to 100°C for 10 minutes in SDS sample buffer and analyzed by western blotting.
-
bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using a centrifugal filter (Millipore; UFC803024). For in vitro EM experiments ...
-
bioRxiv - Microbiology 2020Quote: ... Goat and cow whey protein were purchased from Sigma (USA). The recombined lactoferrin (rLF) ...
-
bioRxiv - Systems Biology 2021Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore Sigma) by wet blotting ...