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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... FLAG and T7 affinity tags were detected with anti-FLAG (F3165, M2 clone, Sigma-Aldrich) and anti-T7 antibody (69522 ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates were subjected to B9-Flag immunoprecipitation using Anti-FLAG M2 Affinity Gel (Sigma) for 2h at 4°C ...
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bioRxiv - Biophysics 2020Quote: Anti-FLAG-M2 affinity agarose gel and 3X FLAG peptide were obtained from Sigma Aldrich, n-dodecyl β-D-maltoside (DDM ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG and HA immunoprecipitations (IP) were carried out by using the ANTI-FLAG® (Sigma) and Anti-HA Affinity Gel (Biotool ...
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bioRxiv - Neuroscience 2022Quote: ... and followed by immunoblotting for Flag (1:1000 Mouse Anti-Flag M2 monoclonal, Sigma F3165) and beta-tubulin (1:1000 Anti-beta-tubulin DSHB Clone E7) ...
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bioRxiv - Molecular Biology 2022Quote: ... referred to as FLAG-C-PHD) were incubated with anti-FLAG M2 beads (Sigma Aldrich) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The AGAL-FLAG was detected with mouse monoclonal Anti-FLAG M2 antibody (F1804, Sigma Aldrich); lysosomes were detected with rabbit polyclonal anti-LAMP2 (custom prepared) ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µL of Flag-agarose gel slurry (Anti-Flag M2 Affinity Gel (Sigma, Cat#: A2220)) was transferred to a 1.5mL microfuge tube washed 2X with 1XTBS and 1X Lysis buffer at 1000 Xg for 30sec ...
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bioRxiv - Microbiology 2022Quote: ... Precleared lysates were subjected to CLAMP-Flag immunoprecipitation using Anti-FLAG M2 Affinity Gel (Sigma) for 2h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... SERK1-FLAG was detected in Western blot assays using monoclonal anti-FLAG (Sigma-Aldrich, F1804).
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bioRxiv - Genetics 2022Quote: ... Flag expression was assessed by Western Blot (Monoclonal ANTI-FLAG® M2 antibody, Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation of FLAG fusion proteins was performed using an anti-FLAG M2 affinity gel (Sigma) by following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The DDB2-FLAG fusion protein was detected using the anti-FLAG HRP (Sigma-Aldrich: A8592) at a 1:5,000 (v ...
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bioRxiv - Neuroscience 2023Quote: ... and the FLAG-Ppil4 was purified by immunoprecipitation with M2 anti-FLAG agarose beads (Sigma) as described above ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... FLAG-LANA mutants (N+C, N, C) were detected with Rabbit anti FLAG (Sigma 7425).
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were cleared by centrifugation at 12000 rpm for 10 minutes and incubated with anti-flag (anti-flag M2 Affinity Gel, Sigma) antibodies covalently attached to agarose 4 hours at 4 °C on a rocking platform ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg of protein per lysate were subjected to anti-FLAG immunoprecipitation using 15 ul of packed magnetic anti-FLAG beads (M2, Sigma) for 4h ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified lysates were incubated with equilibrated anti-Flag M2 affinity gel (ANTI-FLAG M2 Affinity Gel Sigma-Aldrich, A2220) on a rotating wheel for 3 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation reactions were performed using anti-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, F1804, Sigma-Aldrich). The complex of chromatin antibody was captured with protein G beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of protein extracts was performed using a monoclonal anti-Flag antibody covalently attached to agarose (Anti-FLAG M2 Affinity gel, Sigma). Proteins were eluted in 2xNuPAGE LDS Sample Buffer (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were clarified by ultracentrifugation and supernatants were supplemented with NaCl up to a final concentration of 500 mM and subjected to anti-FLAG immunoprecipitation using 100 ul of packed beads magnetic beads (anti-FLAG, M2, Sigma). After 16h beads were washed 5 times with IP buffer and eluted twice with 250 ul of 1 mg/ml 3x-FLAG peptide (Bachem ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Biophysics 2024Quote: ... the blots were blocked with Tris-buffered saline with 0.1% Tween-20 detergent (TBST) + 5% milk and probed using TBST + 5% milk with anti-FLAG (ANTI-FLAG(R) M2-Peroxidase (HRP) by Sigma) or anti-His6X (His HRP conjugate by Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and 1:1,000 anti-FLAG (Sigma; clone M2).
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bioRxiv - Cancer Biology 2021Quote: ... anti-Flag (F7425, dilution 1:1000, Sigma-Aldrich), anti-MYC (05-724 ...
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bioRxiv - Microbiology 2020Quote: ... Anti-FLAG M2 beads (Sigma-Aldrich, Shanghai, China) were added to the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich), anti-TCHP (abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µL anti-FLAG M2 agarose beads (Sigma) were used for the enrichment of FLAG-fusion proteins of each well by incubating with the supernatants overnight at 4℃ on a vertical rotator ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-flag (1:500; Sigma, F1804), mouse monoclonal anti-tubulin (clone DM1A ...
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bioRxiv - Cell Biology 2020Quote: Anti-FLAG M2 affinity gel from Millipore Sigma; XtremeGene9 and Complete Protease Cocktail from Roche ...
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bioRxiv - Cell Biology 2020Quote: ... an anti-M2-FLAG antibody (Sigma-Aldrich, F1804) was used in combination with anti-GAPDH (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... and detected with corresponding antibodies anti-Flag (Sigma), anti-HA (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-FLAG (Sigma-Aldrich, M2; 1/200); rabbit Anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... mouse anti-FLAG (Sigma-Aldrich; catalog no. F1804), mouse anti-HA (EMD Millipore ...
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bioRxiv - Genetics 2021Quote: ... Cell lysate was incubated with anti-FLAG (Sigma) at 4 °C with gentle agitation overnight ...
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bioRxiv - Genetics 2021Quote: ... mouse monoclonal anti-FLAG M2 (Sigma-Aldrich, F1804), and mouse monoclonal anti-MYBPC3 (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2020Quote: ... before incubating with Anti-FLAG M2 resin (Sigma) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... or rabbit anti-FLAG (M2, 1:250, SIGMA), Mouse anti-NC82 (1:50 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 2 µg/ml of anti-FLAG (Sigma), anti-HA (Cell Signaling) ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibodies for anti-FLAG-HRP (Sigma, 1:2500), anti-HA-HRP (Roche ...
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Increasing phagocytosis of microglia through targeting CD33 with liposomes displaying glycan ligandsbioRxiv - Immunology 2021Quote: ... we used anti-FLAG antibody (1:100, Sigma). Briefly cells were permeabilized after fixation step by incubation with 100 μL of 5% goat serum containing 0.1% Triton X-100 for 5 min and were further blocked in 5% goat serum for an additional 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-Flag M2 magnetic beads (Sigma-Aldrich, M8823) were used to immunopurify eF-Piwi from OSCeF-Piwi (https://doi.org/10.1101/2020.12.18.423517) ...
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bioRxiv - Neuroscience 2022Quote: ... immunostained using anti-FLAG antibody (Sigma-Aldrich, #F7425), and imaged by confocal microscopy.
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bioRxiv - Immunology 2022Quote: ... anti-Flag-HRP (Sigma, 1:1000-1:5000), R-anti-Flag (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... and anti-Flag BioM2 (1:2000) (Sigma F9291) overnight at 4°C ...