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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Physiology 2021Quote: ... and then stained for 2 hours in 1 mL of M9 containing 150 μM 2’,7’-dichlorofluorescein diacetate (DCFDA, Sigma) while rotating in the dark ...
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bioRxiv - Microbiology 2020Quote: ... All strains were cultured in either rich (YPD: 1% yeast extract, 2% peptone, and 2% dextrose) or complete synthetic minimal (CSM) media (Sigma) lacking appropriate amino acids with 2% dextrose ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: ... ALP activity was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: ... Skin was minced into small pieces (about 2-3 mm × 2-3 mm) and digested by incubation with 1 mg/mL collagenase V (Sigma) in PBS for one hour with shaking (250 rpm) ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then blocked 1 hr at the room temperature (RT) in PBS with 2% Triton-X100 and 2% normal donkey serum (Sigma), followed by an incubation overnight at 4℃ with primary antibody diluted in the blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... 80 μL of the supernatant was mixed with 20 μL of 0.75M 2-amino-2-methyl-1-propanol buffer (A65182, Sigma-Aldrich) and 100 μL 100 mM p-nitrophenylphosphate solution (71768 ...
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bioRxiv - Bioengineering 2023Quote: ... substrates containing 400 nm diameter pores at a density of 2·106 pores·cm-2 were coated with 100 µL of 1 µg/mL human plasma fibronectin (Sigma-Aldrich) in PBS (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: Equimolar amounts of IgG1-F405L and IgG1-K409R antibodies (1 mg/mL) were mixed with freshly prepared 2-mercaptoethylamine (2-MEA; Sigma) solution (750 mM ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Immunology 2024Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30°C for 18 hours at 200rpm ...
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bioRxiv - Immunology 2024Quote: ... NK cells were pretreated with DMSO or 2-DG (0.5 mM, 1 mM, 2 mM, 5 mM; Sigma-Aldrich, USA), BAY 87 (10 μM ...
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bioRxiv - Biophysics 2024Quote: ... fixed samples were blocked using 2% BSA for 2 h at room temperature and then incubated primary vinculin monoclonal antibody (1:250, V9131, Sigma) for 2 h ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei and actin filaments were stained simultaneously with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI; 1:2000, Sigma-Aldrich, Cat# D9542) and Phalloidin-Atto 647N (1:2000 ...
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bioRxiv - Physiology 2023Quote: ... glycogen phosphorylase was inhibited by IV (jugular vein) injection of 1-(3-(3-(2-Chloro-4,5-difluorobenzoyl)ureido)-4-methoxyphenyl)-3-methylurea (Sigma, 5 mg/kg) one hour prior to the start of a tracer infusion ...
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bioRxiv - Genomics 2024Quote: ... Samples were stained with 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542) for 5-10 minutes at room temperature manually and rinsed three times with 1xTBS with 0.1% (v/v ...
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bioRxiv - Genomics 2024Quote: ... formamide in 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542). Readout probes ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Samples were dehydrated with methanol series (50%, 75%, 95%, 100%, 100%) and overnight incubated in 1:1 methanol : BABB (BenzylAlcohol-BenzylBenzoate 1:2, both Sigma)) ...
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bioRxiv - Immunology 2024Quote: ... Clearing was initiated by incubating samples in a 1:1 mixture of methanol and BABB (benzyl alcohol and benzyl benzoate at a 1:2 ratio, Sigma ref ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-FLAG-tag (CST, rabbit, 1:1000), anti-total-ERK1/2 (CST, rabbit, 1:1000) diluted in PBS containing 1% BSA (Sigma); and secondary antibody anti-rabbit (CST ...
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bioRxiv - Neuroscience 2022Quote: ... and hydroxy-acrylamide (97%; 697931, Sigma) solutions ...
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bioRxiv - Bioengineering 2022Quote: ... and hydroxy-acrylamide (97%; 697931; Sigma) solutions (177:100:23 ratio) ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Developmental Biology 2020Quote: ... Mouse anti-Pax6 (1:100, SantaCruz, sc-53108)and Rabbit anti-Calbindin (1:500, Sigma, c2724-2). Nuclei were stained with 1 μg/ml DAPI (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... MTs were labeled with rat monoclonal antibody YL 1/2 directed against tyrosinated -tubulin (1:1000, Millipore), or rabbit polyclonal antibody against detyrosinated -tubulin (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... blood was diluted 1:2 in PBS and PBMCs were isolated using a Histopaque (1077-1, Sigma) gradient ...
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bioRxiv - Neuroscience 2024Quote: ... vibratome sections were counterstained with 1:1000 DAPI (4ʹ,6-diamidino-2-phenylindole, 1 mg/ml, Sigma) in PBS and incubated for 20 minutes in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... Each RNA sample was mixed with a 1:1 volume of 2-Propanol (Sigma-Aldrich, 190764-4L) and 0.5 µL Glycogen ...
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bioRxiv - Microbiology 2023Quote: ... acetic acid and distilled water (2:1:1 [vol:vol:vol]) mobile phase and stained using 0.3% of N-napthyl ethylenediamine dihydrochloaride (Sigma) as described previously (17) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% P/S and 1% GlutaMax and 2 μM Cytosine beta-D-arabinofuranoside (AraC; Sigma, #C1768-100MG) to inhibit glial cell growth ...
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bioRxiv - Plant Biology 2020Quote: ... and anti-α-tubulin antibodies (clone B-5-1-2, Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... and this mixture then mixed 2:1 with Fast Green solution (Sigma) and microinjected into the neural tubes of the embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... with BMP4 (1 ng/ml, R&Dsystem) and Thiazovivin (2 microM, Millipore). On day 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... with BMP4 (1 ng/ml, R&Dsystem) and Thiazovivin (2 μM, Millipore). On day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... selection was performed with 1–2 μg/mL puromycin (P8833; Sigma-Aldrich) or 2–3 μg/mL blasticidin (022-18713 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by a 1-2-day photosynthesis inhibitor diuron treatment (Sigma-Aldich) at 50 µM50,51 ...
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bioRxiv - Bioengineering 2021Quote: ... 1% v/v aprotinin (2 mg/mL, Sigma Aldrich, Cat# 616370-M) was added to all media to prevent fibrin gel degradation ...
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bioRxiv - Bioengineering 2020Quote: ... and 0.25 mg·ml−1 L-ascorbic acid 2-phosphate (AA2P, Sigma, A8960)) overnight to allow for protein adsorption.
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bioRxiv - Neuroscience 2021Quote: ... Anti-TYR-TUBULIN clone YL1/2 (1:1000; MAB1864-I, Sigma-Aldrich) antibodies were diluted in a solution of 0.5% BSA ...