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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Developmental Biology 2024Quote: ... Live larvae (3-10 dpf) were immobilized in tricaine and mounted in 1-2% low gelling agarose (Sigma: A9414) on 0.17 mm coverslips and imaged on a Stellaris DMI8 laser scanning confocal microscope at 1024×1024 resolution using the same acquisition settings with a 25x water immersion objective.
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bioRxiv - Microbiology 2024Quote: ... 2 ug mL-1 1-[(5S)-4,5-Dihydro-5-phenyl-1H-pyrazol-1-yl]-2 (GSK’963) (Sigma-Aldrich # SLM2376- 25MG), 2 ug mL-1 N-(6-(isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine hydrochloride (GSK’872 ...
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bioRxiv - Cell Biology 2022Quote: ... were treated with 0.1 M NaOH solution for 5 min to increase the surface area of the coverslip and enable the subsequently added 3-(trimethoxysilyl)propyl methacrylate (cat. no. 440159, Sigma-Aldrich) to attach ...
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bioRxiv - Bioengineering 2024Quote: Hydrogels for cell culture experiments were fabricated by placing 90-µL drops of precursor solution between a 3-(trimethoxysilyl)propyl acrylate (Sigma-Aldrich) silanated 18-mm glass coverslip and a glass slide treated with SigmaCote (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... The surfaces of the glass slides were then modified with methacrylate groups by immersing them in a solution containing 3-(trimethoxysilyl)propyl methacrylate (TMSPMA; Sigma-Aldrich) and Toluene (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... 2-3-week-old plants were treated with 150µM DCMU (D2425-100G, Sigma), kept for 1 hour in the dark and then exposed to high light (HL ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000037282 (shRNA Trim39#2) and TRCN0000438509 (shRNA Trim39#3) were from Sigma-Aldrich. Lentiviral particles were produced as previously described (Iréna Lassot et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Neuroscience 2021Quote: ... MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] were acquired from Sigma (USA). Purified water was prepared with a Milli-Q purification system from Millipore (Milford ...
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bioRxiv - Neuroscience 2020Quote: ... 0.75 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) was added to culture medium for 2 h ...
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bioRxiv - Biophysics 2020Quote: ... and 2 mg/ml N-(3-dimethylaminopropyl)-N′-ethylcarbodiimidehydrochloride (EDC, Sigma-Aldrich, 03450) in 50 mM 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μL 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) solution (Sigma) was added to each well according to manufacturer’s instructions and analysed in a spectrophotometer at 580 nm ...
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bioRxiv - Immunology 2021Quote: ... mtDNA depletion was induced by 100 μM 2′,3′ dideoxycytidine (ddC, Sigma-Aldrich) in medium supplemented with uridine and sodium pyruvate ...
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bioRxiv - Immunology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Sigma, 28718-90-3, St Louis, MO, USA). The results were recorded using Zeiss LSM780 confocal system (Zeiss ...
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bioRxiv - Biophysics 2020Quote: ... MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, p# M5655, Sigma-Aldrich) was dissolved in Phosphate Buffer Solution (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... the reagent 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) was added to the cells and incubated for 4 hours at 37 °C (5 % CO2) ...
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bioRxiv - Neuroscience 2022Quote: ... buffered with 3 mM N-2-hydroxyethylpiperazine-N’-ethanesulfonic acid (HEPES, Sigma Chemical) and pH adjusted to 7.65 at 15°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The MTT compound (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) was dissolved in RPMI 1640 without phenol red ...
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bioRxiv - Molecular Biology 2022Quote: ... growth medium was supplemented with 100 μM of 2′,3′-dideoxycytidine (Sigma; D5782) or 50 ng/ml of ethidium bromide ...
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bioRxiv - Neuroscience 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2, Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich), and then incubated for 1 hour on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Phosphatase inhibitor cocktail 2 (#P5726) and cocktail 3 (P0044) were from Sigma-Aldrich. Glutathione Sepharose 4B (#17-0756-01 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in a solution of 2/3 dichloromethane (DCM; Sigma-Aldrich), 1/3 MetOH ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium Bromide – MTT (Sigma-Aldrich), MitoTracker probe (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 3-(4,5-dimethylthiazol-2-Yl)-Diphenyltetrazolium Bromide (MTT, CT01-5, Sigma-Aldrich, UK) stock solution was diluted in F12 medium without phenol red (1:5) ...
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bioRxiv - Molecular Biology 2023Quote: ... fed every day and split every 2-3 days using Accutase (A6964, Sigma).
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bioRxiv - Biochemistry 2023Quote: Rosetta-gami 2 competent cells (Novagen / Millipore-Sigma, USA; cat. no. 71350-3)
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bioRxiv - Biochemistry 2023Quote: Rosetta-gami 2 competent cells (Novagen / Millipore-Sigma, USA; cat. no. 71350-3)
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bioRxiv - Neuroscience 2023Quote: ... The animals received unilateral injections totalling 3×2 μg of 6-OHDA (Sigma Chemical CO ...
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bioRxiv - Molecular Biology 2024Quote: ... phosphatase inhibitor cocktails 2 and 3 (100uL/10mL, Sigma Cat #P5726 and P0044)) and lysates were cleared by centrifugation at 17,000g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO, USA) to prevent degradation of the protein of interest ...
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bioRxiv - Neuroscience 2024Quote: ... phosphatase inhibitor Cocktails 2 and 3 were from Sigma Aldrich (St. Louis, MO). Mouse anti-Rac1 antibodies (#ARC03) ...
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bioRxiv - Molecular Biology 2024Quote: ... fed every day and split every 2-3 days using Accutase (A6964, Sigma).
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bioRxiv - Bioengineering 2022Quote: ... Polymerization of 1 M monomer was performed with 4 mol% N,N’-methylene-bis-acrylamide (Sigma-Aldrich) as a crosslinker with 0.1% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... We dissolve 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) powder (Sigma Aldrich) in HEPES buffer (pH 7.18 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the coupling reagent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich). Stock solutions were prepared in phosphate-buffered saline at concentrations of 140 mM (PDH ...
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bioRxiv - Microbiology 2024Quote: ... CPSs were incubated with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, Sigma-Aldrich). The mixture was then ultrafiltered to remove extra DMTMM and mixed with carboxyl latex beads (2 μm ...
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bioRxiv - Biophysics 2021Quote: ... extraction solution #1 was replaced by extraction solution #2 (2% Triton X-100, 1% CHAPS hydrate; Sigma Aldrich, Germany) for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... separated by SDS-PAGE with 4-12% Bis-tris gels (Sigma), and transferred to low fluorescence PVDF membranes with the Trans-Blot Turbo Transfer System according to manufacturer’s instructions (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed with PBS and incubated with 100 µM 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2NBDG) (Sigma Cat# 186689-07-6) containing RPMI1640 (HiMedia Cat# AT150 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...
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bioRxiv - Immunology 2024Quote: ... T lymphocytes were adjusted at 50.000 cells/ml in their own culture medium and 0.5 ml of the cell suspension were transferred to 1.5 ml microcentrifuge tubes and incubated at 37°C for 2 hours in the absence or presence of metabolic inhibitors: 50 to 1 mM 2-deoxyglucose (2-DG) (Sigma, catalog: D8375-5G), 200 µM etomoxir (Sigma ...
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bioRxiv - Zoology 2020Quote: ... 3-methyl-but-2-enyl acetate and (2E)-hex-2-enyl acetate were confirmed with analytical standards from Sigma-Aldrich (St. Louis, MO). For the analysis of CHCs ...