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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... (2) LPS group: Mice received intranasal inhalation of 20 uL LPS (1 ug/uL, from Escherichia coli 0111:B4, Sigma) and i.v ...
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bioRxiv - Biochemistry 2022Quote: Streptococcus pyogenes Cas9 wild type protein and the nuclease dead mutant (D10A, H840A) were both recombinantly expressed for 16 hours at 18 °C in Escherichia coli Rosetta 2 (DE3) (Novagen) N-terminally fused to a hexahistidine affinity tag ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml EGF (Sigma, E 4127) and 100 μl LIF(Millipore ...
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bioRxiv - Biochemistry 2020Quote: Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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bioRxiv - Molecular Biology 2020Quote: Gamma secretase inhibitor Compound E (Millipore Sigma) was dissolved in DMSO and diluted 1:1000 in RPMI media to a final concentration of 1 μM ...
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bioRxiv - Systems Biology 2021Quote: ... 10 μM E-64 (Sigma-Aldrich # 324890), 1.45 μM pepstatin (Sigma-Aldrich # P5318) ...
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bioRxiv - Microbiology 2020Quote: ... The cathepsin inhibitor E-64d (Sigma-Aldrich), the serine protease inhibitor camostat mesylate (Abcam) ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... Rabbit anti-CENP-E (C7488, Sigma Aldrich), diluted 1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... Ergosterol obtained from Sigma-Aldrich (E-6625), cholesterol ester ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.5 mg/mL ProNase E (Sigma P6911), 62.5 U/mL DNAse (Applichem ...
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bioRxiv - Biochemistry 2020Quote: ... pepstatin and E-64 (Sigma Aldrich, USA). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Culture medium was Williams’ Media E (Sigma) supplemented with nicotinamide (12 mmol/L) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1x β-mercaptoethanol (Millipore, ES-007-E), 1µM PD98059 (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μM E-64 (Sigma, E3132) for protease inhibition ...
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bioRxiv - Immunology 2024Quote: ... and stained with H&E (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 μg/ml E-64 (Sigma-Aldrich), 5 mM EDTA (Calbiochem) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3.4 mM vitamin E (Sigma-Aldrich) (45) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20-hydroxyecdysone (20-E) (H5142; Sigma Aldrich) was resuspended to an initial stock concentration of 10 mM in ethanol.
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bioRxiv - Microbiology 2024Quote: ... with 10X E-Media (DMEM, Millipore-Sigma, #D7777-10L ...
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bioRxiv - Microbiology 2024Quote: ... and ethambutol (E) were purchased from Sigma. Pyrazinamide (Z ...
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bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... coli (Novagen and Supplementary file 5) were transformed with pMH-HS-Sumo-dOCRL168-509 vector (KanR ...
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bioRxiv - Microbiology 2021Quote: ... coli strain Rosetta2 (DE3) pLysS (Novagen). For each purification procedure ...
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bioRxiv - Biophysics 2021Quote: ... coli Rosetta2 (DE3) pLysS cells (Novagen) were transformed with pET-21a plasmids encoding for PFD1 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli Rosetta (DE3) pLysS cells (Sigma) for protein overexpression ...
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bioRxiv - Immunology 2022Quote: ... coli (Novagen, Merck KGaA, Darmstadt, Germany) for subsequent recombinant protein production following induction with 1 mM IPTG (Carl Roth ...
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bioRxiv - Molecular Biology 2021Quote: ... coli strain Rosetta2(DE3)pLacI (Novagen), and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... coli 3 BL21 (DE3) Codon+ (Novagen) in 1 L of auto-inducible terrific broth media (ForMedium AIMTB0260 ...
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bioRxiv - Immunology 2022Quote: ... LPS (Escherichia coli O127:B8, Sigma), cGAMP (InvivoGen) ...
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bioRxiv - Biophysics 2021Quote: ... coli BL21 (DE3) cells (Novagen -USA) and purified by metal-affinity and SEC ...