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Citations for Millipore Sigma :
1001 - 1050 of 7239 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Ultra centrifugal filters for protein concentration (Millipore-Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μL of protein G Sepharose (SIGMA), previously blocked for 1 hr with lysis buffer containing 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein G agarose beads (Millipore # 16-201) were used to pull down the antibody-protein complex ...
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bioRxiv - Cancer Biology 2023Quote: ... and horse myoglobin as standard proteins (Sigma). All spectra were processed and analyzed using MassLynx 4.1 software.
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 µl of protein A-sepharose (Sigma) were added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... Protein was transferred to PVDF membranes (Millipore) using the TransBlot Turbo semi-dry apparatus (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Protein G agarose (Millipore, Temecula, CA, USA) and anti-myc antibody (Abmart ...
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bioRxiv - Microbiology 2023Quote: ... conjugated with protein G beads (Sigma-Aldrich) for 16 hours at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 nM Cas9 protein (E120020-250ug, Sigma), and 500 nM circular donor plasmid were mixed in the injection buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... protein G- sepharose beads (Sigma, Ref. P3296), GFP-Trap MA beads (Chromotek ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (Millipore) and blocked in 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-TMEM119 (Transmembrane Protein 119; Sigma Prestige Antibodies ...
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bioRxiv - Microbiology 2023Quote: ... or control protein OVA albumin (Sigma/A5503) were mixed with NL63 virus at MOI 0.1 (1:1 ...
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bioRxiv - Plant Biology 2023Quote: ... After electroblotting proteins on nitrocellulose membrane (Millipore), protein blot analysis was performed using antiserum with specific antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... PureProteome Protein A magnetic beads (30µL; Millipore) for 2 hr at 7 rpm at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Protein G agarose beads (Millipore # 16-201) were used to pull down the antibody– protein complex ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μL of protein G Sepharose (Sigma) with Casp-8 (C20 ...
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bioRxiv - Plant Biology 2023Quote: ... protein expression in E.coli Rosetta cells (Novagen) was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Molecular Biology 2024Quote: ... and Protein G agarose (Millipore, 16-201) beads were blocked with 3%BSA in dilution buffer before adding to cell lysate for 1.5h at 4°C with rotation (50 μL/sample beads resuspended in 50 μL/sample dilution buffer ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Cancer Biology 2021Quote: Protein extracts were separated from each cell line with RIPA protein lysis buffer (R0278, Sigma, St Louis, MO, USA) containing 1X protease cocktail (P8340 ...
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bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of protein from the different S1 fractions was first treated with PureProteome Protein G Magnetics Beads (Millipore) for contaminant IgG depletion and then directly applied to dry nitrocellulose ...
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bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Developmental Biology 2019Quote: ... The HDAC6 protein was immunoprecipitated from total protein lysates (0.25 mg) of cells treated with different compounds using protein A agarose beads (Sigma). The HDAC6 protein bound beads were used as enzyme source and was incubated with the HDAC assay buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and protein diluted to 1mg/ml 1ml of protein lysate was precleared with 20μl of protein G sepharose bead slurry (Sigma) for 1 hour to remove native immunoglobulins ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein was estimated using the bicinchoninic acid protein estimation method (BCA, B9643, Sigma-Aldrich). We separated a protein sample (80µg ...
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bioRxiv - Paleontology 2023Quote: Information for the protein standards purchased for the ToF-SIMS analyses are as follows: type-1 collagen protein (Sigma, CAS ...
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Genomics 2022Quote: ... in a 12-well cell culture plate coated with Human recombinant laminin 511 (BioLamina, Cat. LN511-0202) and fixed in 1xPBS with 3.7% formaldehyde (Millipore Sigma, Cat. F8775-25ML) for 10 mins and then permeabilized in ice-cold 70% (vol./vol. ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G and Protein A Dynabeads (1:1 mixed) were blocked with 1mg/ml denatured yeast tRNA (Sigma-Aldrich, R5636) and 1mg/ml of BSA (NEB ...