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Citations for Millipore Sigma :
1001 - 1050 of 3554 citations for FGF 21 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Immunology 2024Quote: ... whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich). The plate was revealed with OPD/H2O2 HRP-chromogenic mix substrate and the absorbance was measured at 492 nm ...
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bioRxiv - Pathology 2021Quote: ALI cultures were fixed for 15 minutes on day 21-32 in 4% paraformaldehyde and stained for α-tubulin (Sigma-Aldrich, MAB1864; 3 μg/mL), MUC5AC (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... evaporated to dryness under flowing air and redissolved in ethanol with 300 µL of 21% (v/v) sodium ethoxide (Sigma-Aldrich, St. Louis, MO, USA). The reaction was gently vortexed every 5 min for 30 min in the fume hood ...
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bioRxiv - Plant Biology 2022Quote: ... genotypes were grown on MS medium supplied with or without the indicated concentration of 1-aminocyclopropane-1-carboxylic acid (ACC) (Sigma-Aldrich, CAS 22059-21-8).
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 21 days-after-flowering (DAF) sunflower seeds using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Cancer Biology 2021Quote: ... and Human specific Ki67 (MAB4383, 1:500, Millipore), followed by secondary antibody Envision+ anti-mouse labeled-polymer (Dako/Agilent) ...
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10 μg/ml recombinant human insulin (Sigma). BT474 cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: F4883-500MG) were purchased from Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: T6884-100UN) and Fibronogen ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix) ...
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bioRxiv - Cell Biology 2022Quote: ... Human cells were pretreated with glycine (Millipore, 104201) at a concentration of 50 mM for at least 10 minutes before stimuli was added.
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bioRxiv - Bioengineering 2022Quote: ... human [Leu15]-gastrin I (10 nM; Millipore Sigma), N-acetyl cysteine (500 μM ...
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bioRxiv - Immunology 2022Quote: ... and 0.5% recombinant human serum albumin (Sigma Aldrich). Microfluidic droplets were generated as water-in-oil emulsions using a co-flow of aqueous phases ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Thrombin from human plasma was obtained from Millipore-Sigma (Cat ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 20% Human serum (Sigma-Aldrich, UK), and used up to passage 4.
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bioRxiv - Bioengineering 2019Quote: ... and human plasma fibronectin (Millipore Sigma, Catalog #FC010) were conjugated to fluorophores using the DyLight™ 488 Microscale Labeling Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... and 5 IU of human chorionic gonadotropin (Sigma) were injected into 3 week old B6C3F1/J female mice by intraperitoneal injection to stimulate superovulation ...
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bioRxiv - Molecular Biology 2019Quote: ... Human α-thrombin (20 nM, T6884, Sigma, USA) and human α-FXIIa (20 nM ...
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bioRxiv - Immunology 2019Quote: ... Peroxidase-labeled anti-human IgG (A8667, Sigma-Aldrich) was applied at 1:60,000 for 1 h at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... 200nL 0.1µg/µL human noggin protein (Sigma H6416) was injected into the blastocoel of embryos at stage 8 ...
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bioRxiv - Cell Biology 2019Quote: ... and human-EGF (50 ng/ml, Sigma Aldrich), as published 60 ...
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bioRxiv - Physiology 2019Quote: ... 5 IU human chorionic gonadotropin (hCG, Millipore, 230734), after which they were immediately put in mating with fertile 2-4-month old C57Bl/6N males ...
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bioRxiv - Microbiology 2019Quote: ... Complement was obtained from lyophilized human sera (Sigma) reconstituted in sterile water as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of human AChE (Sigma, catalogue number C0663) and BuChE (Sigma ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of human CES1 (Sigma, catalogue number E0287) and CES2 (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855 ...
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bioRxiv - Bioengineering 2020Quote: ... sterile fibrinogen from human plasma (Millipore Sigma, 341576) was dissolved in sterile PBS (Corning ...
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bioRxiv - Biochemistry 2020Quote: 400 μL human male serum (Sigma; cat. #H4522) was incubated at 37 °C for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... with 6.3 µM human holo Tf (Sigma-Aldrich) in 0.1 M carbonate buffer (1 ml ...
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bioRxiv - Bioengineering 2021Quote: ... Polyclonal IgM isolated from human serum (Sigma Aldrich) was used as control ...
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bioRxiv - Microbiology 2020Quote: ... 10 nM [Leu15]-gastrin I human (Sigma-Aldrich), 10% (vol/vol ...