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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for 6 methylpyridine 2 4 diol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Next the slides were mounted with a coverslip using Mowiol (6 g glycerol, 2.4 gr polyvinylalcohol 4-88 (Sigma, 81381), 6 ml MQ and 12 ml 0.2 M Tris HCL pH 8.5).
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... RIPK3 inhibitor GSK 872 (N-(6-(Isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine) was purchased from Millipore Sigma and frozen stocks were maintained in DMSO ...
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.
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bioRxiv - Developmental Biology 2021Quote: ... inactivated with 10 µg/ml mitomycin C and seeded at 4 × 105 per 6 cm dish) in DMEM/F12/KSR medium (D-MEM/F12 (Sigma) supplemented with 20% KSR additive ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
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bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
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bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Neuroscience 2024Quote: Pharmacological experiments were carried out on PCs during simultaneous somatic and dendritic recordings after 10 minutes of control recording using ACSF with the following drugs: 20 µM 4- (N-ethyl-N-phenylamino)-1,2 dimethyl-6-(methylamino)pyrimidinium chloride (ZD7288) (Sigma-Aldrich), or 1 µM TTX ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT cells were plated in 10-cm dish with culture medium containing only blasticidin S following co-transfection with Flp recombinase expressed plasmid pPGKFLPobpA and tetracycline-responsive plasmid pcDNA5FRT/TO containing TDP-43 Q331K or pcDNA5FRT/TO only as isogenic sham control in the ratio 6:4 using PEI (polyethyleneimine; Sigma) transfection method ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... i.p.) young adult (4-week-old) C57BL/6 male mice and transferred to ice-cold DMEM/F12 media (Sigma Aldrich) in 6 well plates ...
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bioRxiv - Cell Biology 2023Quote: ... Gene mutation was induced 4 days after organoid passage during 6 days with 400 ng/ml doxycycline (D9891, Sigma-Aldrich). When indicated ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted and purified mGlu5-5M receptor and mGlu5-5M_W785A receptor mutant bound to quisqualate and PAM were concentrated to 5-6 mg/ml using Amicon Ultra-4 centrifugal concentrator 100 kDa (Millipore) for cryoEM grid preparation and analysis.
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Microbiology 2022Quote: ... diluted in DMSO and 6-Thioguanine (6-TG) (Sigma-Aldrich) diluted in 1M NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... and glucose-6-phosphate dehydrogenase (G-6-PDH; Sigma-Aldrich). For enhanced chemiluminescence detection (ECL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Dextran T-500 (Sigma-Aldrich; 6 mL, 6% in saline) was added to the cells and filled up to 50 mL with 0.9% saline ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/mL 6-animopenicillanic acid (6-APA) (Sigma-Aldrich), 5 mg/mL 7-aminocephalosporanic acid (7-ACA ...
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bioRxiv - Neuroscience 2024Quote: ... The neurotoxin 6-hydroxydopamine-HBr (6-OHDA; Sigma Aldrich AB) was dissolved in 0.1% ascorbate-saline at the concentration 4 µg/µl (freebase) ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Vital dye staining of lateral line hair cells of the 4 dpf embryos was performed using DASPEI (2-(4-(dimethylamino) styryl)-N-ethylpyridinium iodide) (Sigma-Aldrich, USA, D0815), 0.8 µg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... pH 6 (Sigma) at 95 °C for 20 minutes then room temperature for 20 minutes prior to staining ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 cells were plated in 6-well poly (2-hydroxyethyl methacrylate) (poly-HEMA, Sigma-Aldrich, St. Louis, MO, USA)-coated plates for the indicated amount of time in the figure legends ...
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bioRxiv - Immunology 2019Quote: ... spleens from C57BL/6 mice were cut to smaller pieces and digested with Collagenase D (2 mg/ml, Sigma) For 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were fasted for 6 hours prior to IP injection of 2 g/kg 20% D-glucose (Sigma-Aldrich) in water with a 26G needle ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were then spun down (2 min with 1000 rpm) and resuspended in 6 ml serum-free medium (Sigma Cat# ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... at an age of 6-10 weeks by a single intra peritoneal injection of 2 mg Tamoxifen (Sigma; T5648) in corn oil (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Adult female flies aged 2–6 days post eclosion were head-fixed to a custom mount using eicosane (Sigma). Cuticle ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Cell Biology 2024Quote: ... 561 and 640 nm laser lines were used for excitation of DAPI (4rn,6-Diamidino-2-phenylindole, Sigma-Aldrich), Alexa Fluor 555 and Alexa Fluor 647 respectively ...
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bioRxiv - Neuroscience 2019Quote: ... whole brains of females (n = 6) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs (12×106 cells/mL) and MSCs (6×105 cells/mL) were suspended in EBM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... the acid insoluble fraction was incubated for 18 hours at 4°C with 500 μL of 6 M guanidine hydrochloride/100mM TRIS buffer (pH 7.4, Sigma-Aldrich, U.K.). The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was immobilized on a nitrocellulose membrane and then probed with antibodies against (6-4)PPs (Cosmo Bio NM-DND-002) or ssDNA (Millipore MAB3034). Chemiluminescence was detected as for protein immunoblotting ...