Labshake search
Citations for Millipore Sigma :
1001 - 1050 of 9019 citations for 4 Acetoxy 4' fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... 4-(4,6- dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) was purchased from Sigma Aldrich (Cat: 74104). Nano-HPLC solvents were LC/MS grade (LiChrosolv® ...
-
bioRxiv - Bioengineering 2024Quote: We used tartrazine (T0388, industrial grade, ≥85%) and 4-aminoantipyrine (4-AA, A4382, reagent grade) sourced from Sigma Aldrich, along with low-melting-point agarose (A9414 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Ovary of both medaka and trout were fixed overnight at 4°C by 4% paraformaldehyde (PFA) in 0,01 M phosphate buffer saline pH 7.4 (PBS, Sigma-Aldrich P4417). Ovaries were washed in PBS and conserved in PBS + 0.5% (w/v ...
-
bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by 4% paraformaldehyde in 0.1 M phosphate buffer (4% PFA-PB; pH 7.4; Sigma-Aldrich, St Louis, MO, USA). Lumbar spinal cords were collected and placed into 4% PFA-PB for 2 hours at 4 °C ...
-
bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sma-CreERT2 or Cx40-CreERT2 males were crossed with R26-Confetti females and low doses of 4-Hydroxytamoxifen (4-OHT H6278, Sigma) was injected intraperitoneally to pregnant females in a single injection ...
-
bioRxiv - Biochemistry 2021Quote: ... and 4) the substrate solution (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate, Sigma-Aldrich, St. Louis, MO). Other details were as described previously [Duk M. ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were subsequently fixed in paraformaldehyde (PFA) 4% with 4% sucrose for 10 min and permeabilized with 0,1% Triton X100 (Sigma-Aldrich) for 5 min ...
-
bioRxiv - Neuroscience 2020Quote: 4% hydrogel monomer solution (HMS) was prepared for perfusion by mixing 40% (w/v) acrylamide (4% final concentration; V900845, Sigma), 2% (w/v ...
-
bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated at 37°C for 30 minutes prior to fixation with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Extracted membranes were separated from insoluble debris by centrifugation (130,000 × g for 1 h at 4 °C) and incubated for 2h with 4 mL of Ni2+-NTA beads (Novagen) equilibrated in buffer III (25 mM Tris-HCl ...
-
bioRxiv - Neuroscience 2020Quote: Cells were washed with phosphate buffered saline (PBS) and fixed for 30 min at room temperature with 4% paraformaldehyde (containing 4% sucrose; Sigma). Cells were permeabilized in 0.5% NP40 in PBS for 5 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... and 0.26g (12.48 x 10−4 mol, 1:4 stoichiometric ratio) cross-linking agent tetraethyl orthosilicate (TEOS – Sigma-Aldrich, 131903) were mixed at 3500rpm for 1 min using a DAC 150FV2-K speedmixer ...
-
bioRxiv - Cell Biology 2021Quote: ... Chromatin was pelleted by spinning 5 minutes at 10,000 g at 4°C and subsequently incubated for 1 hour at 4°C in HEPES buffer containing 500 units of Benzonase (Millipore) and 2 µg Pol II antibody (ab5095 ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
-
bioRxiv - Neuroscience 2020Quote: ... NestinCreERT2 - /+ TDT +/+ IR fl/fl generated secondary spheres were allowed to grow for 4 DIV and then 0.5 µM of 4-hydroxy tamoxifen (Sigma-Aldrich) or vehicle (ethanol ...
-
bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
-
bioRxiv - Neuroscience 2020Quote: ... Induction of recombination in Ai9 mice electroporated with Cre inducible plasmids was achieved by intraperitoneal injection of pregnant dams with the faster-acting and shorter-lived tamoxifen metabolite 4-hydroxy-tamoxifentamoxifen (4-OHT) (Sigma) (1 mg/20 g of body weight ...
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: Cells were seeded on coated glass cover (Assistent) slips 2 days before fixation with 4 % paraformaldehyde (Sigma, 30525-89-4) for 15 minutes ...
-
bioRxiv - Immunology 2022Quote: ... 0.6μL was deposited on a thin layer of an air-dried saturated solution (0.6μL) of the matrix alpha-cyano-4-hydroxycinnamic Acid (4-HCCA, Sigma Aldrich, France) in pure acetone ...
-
bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... the brain was kept in 4% PFA overnight at 4□°C and then placed into PBS containing 0.02% sodium azide (Sigma S8032). The brain was serially sectioned into 50 μm transverse slices on a Campden Instruments 5100mz vibratome ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were stained with 12.5 μg/ml Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophos-phate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate (Sigma Aldrich ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and then 4 fractions of 4mL were concentrated 8 times using 10 kDa Amicon Ultra-4 10K Centrifugal filters (Millipore) after centrifugation (20 min ...
-
bioRxiv - Microbiology 2020Quote: ... Flagella preparations were incubated at 70°C for 15 min then filtered by centrifugation at 5000 × g at 4°C for 30 min using 100 kDa 4 ml filter units (Millipore). Filtrates were confirmed by size-exclusion HPLC to be exclusively monomeric.
-
bioRxiv - Microbiology 2021Quote: ... GM130 (12480, cell signaling), PI(4)P (Z-P004), PI(4)KB (06-578, Milipore) and anti-actin (A5316, Sigma). Secondary antibodies were as followed ...
-
bioRxiv - Neuroscience 2020Quote: ... Srff/f and Srff/fCaMKCreERT2 pups were intraperitoneally injected every other day with three doses of 0.25 mg 4-hydroxytamoxifen (4-OHT; Sigma, #7904) or one dose of 0.75 mg of this compound in sunflower oil ...
-
bioRxiv - Genetics 2020Quote: ... and the colour reaction was carried out with NBT/BCIP (4-nitroblue tetrazolium chloride/bromo-4-chloro-3-indolyl phosphate; Sigma). Sections were dehydrated and mounted with Vectamount (Vector Laboratories ...
-
bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: Oocyte live cell imaging was done in 4-well Labtek chambers (ref. 155383) in 4 μl drops of M16 medium covered with mineral oil (Sigma) in a 5% CO2 environmental microscope incubator at 37 °C (Pecon) ...
-
bioRxiv - Cell Biology 2021Quote: Lgr5-EGFP-IRES-creERT2/RCL-tdT small intestinal organoids were treated in vitro with 100 nM of 4-Hydroxitamoxifen (4-HT) (Sigma) for 48 h to induce the expression of tdTomato in Lgr5+ cells ...
-
bioRxiv - Biochemistry 2022Quote: ... The IMAC pool was diluted 1:4 in 50 mM Tris HCl pH 8 and applied to 4 mL DEAE-Sepharose (Sigma). After washing with 20 mM Tris HCl ...
-
bioRxiv - Neuroscience 2022Quote: ... Ai14 double transgenic mice were generated and received virus injection of rAAV9-N-RAM- d2tTA-eYFP with an identical schedule as described above except of 4-OHT injection (50 mg/kg, 4-Hydroxytamoxifen, H7904, Sigma) immediately after fear conditioning ...
-
bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
-
bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
-
bioRxiv - Microbiology 2022Quote: ... was previously isolated on the International Streptomyces Project 2 medium (ISP2, dextrose 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St ...
-
bioRxiv - Systems Biology 2024Quote: ... and kept at 4°C for a maximum of 4 hours until plasma was separated via centrifugation (2600g for 10 minutes at 4°C) (Sigma 3-16KL ...
-
bioRxiv - Systems Biology 2024Quote: ... and kept at 4°C for a maximum of 4 hours until plasma was separated via centrifugation (2600g for 10 minutes at 4°C) (Sigma 3-16KL, Sigma Laboratory Centrifuges ...
-
bioRxiv - Plant Biology 2024Quote: ... AGPs were finally detected using the following substrates: 4 mg of 5-bromo-4-chloro-3-indolylphosphate (BCiP; Sigma, USA) in 1 mL water and 9 mg of nitro-blue tetrazolium (NBT ...
-
bioRxiv - Microbiology 2024Quote: ... Tissues were fixed in 4% paraformaldehyde for 24 hours at 4 °C and washed with Dulbecco’s phosphate-buffered saline (DPBS, Sigma, USA). After dehydrated through a graded ethanol series to absolute ethanol ...
-
bioRxiv - Neuroscience 2024Quote: ... neurons were fixated at DIV42 with 4% paraformaldehyde/4% sucrose for 15 min and permeabilized with 0.2% Triton X-100 (Sigma Aldrich) in PBS for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... Rosa-Tomato mice in diestrus cycle were injected intraperitoneally with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich, #H6278, St. Louis, MO) to induce CreER recombinase activity ...
-
bioRxiv - Immunology 2024Quote: ... or alternatively plus IL-4 (4ng/ml) or IL-4 and lipopolysaccharide (LPS) (10μg/ml) (Sigma-Aldrich, St. Louis, MO) for 2.5 days.
-
bioRxiv - Microbiology 2023Quote: ... cells were fixed overnight in 4% paraformaldehyde in PBS at 4°C and plated onto 0.1% poly-L-lysine (Sigma-Aldrich) coated cell slide ...
-
bioRxiv - Cancer Biology 2023Quote: ... in vitro culturing was continued in fresh medium containing 500 nM 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich, St. Louis, MO), which was changed daily ...
-
bioRxiv - Microbiology 2023Quote: ... After addition of 250 µL 4× lysis buffer (4% SDS, 40 mM tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich, C4706), 160 mM chloroacetamide (CAM ...
-
bioRxiv - Developmental Biology 2023Quote: ... and in vitro assays on SK-MEL-28 cells were as follows: 4-Hydroxyanisole (4-HA, Sigma-Aldrich, MO, USA), the canonical Wnt pathway antagonist IWR-1 (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... solution for 4 h at 60°C and then dialyzed at 4°C against pure water for 4 days using cellulose dialysis tubes (MWCO 3.5 kDa, Sigma Aldrich). Subsequently ...