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Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Immunology 2022Quote: ... and then incubated overnight at 4°C with 1:1000 dilution of primary antibody - either anti-p52/p100 (Millipore Sigma Cat#05361) or anti-β-Actin (Cell Signaling Technology Cat#3700) ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Molecular Biology 2019Quote: Recombinant rcCBP2 was run on SDS-PAGE and subjected to western blotting using anti-hexa histidine HRP (horse radish peroxidase) conjugated antibodies (Sigma; 1:2000) to confirm identity of the purified protein.
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bioRxiv - Bioengineering 2019Quote: ... MA) and the secondary antibody used was HRP-conjugated goat polyclonal anti-mouse IgA (1:500; A4789; Sigma Aldrich; St. Louis, MO). HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... 5 × 106 cell equivalents were analyzed on 10% PAA / SDS gels and sequentially probed with anti-HA antibodies (Sigma, 3F10, 1:3000), anti-Protein A antibodies (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Molecular Biology 2021Quote: The following antibodies were used for Western blotting at the indicated dilution: anti-FLAG-HRP conjugate (Sigma M2 A8592-1MG, 1:5000); anti-MBTD1 (Abcam ab116361 ...
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining.
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-calbindin (1:1000, mouse, Sigma Aldrich, #C9848). After 3 rinses with PBS for 10 min ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in a phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... For KIF18A localization analyses the following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (MAB1864; Sigma Aldrich), rabbit anti-KIF18A 1:100 (A301-080A ...
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bioRxiv - Genetics 2019Quote: ... 0.1%SDS and 1% Tween 20 and re-stained for the loading using the anti-glucose-6-phosphate dehydrogenase antibody (Millipore, cat #A9521). Blots were again imaged on ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were analysed using 4-12% Bis-Tris polyacrylamide gels in MOPS buffer followed by Coomassie staining or western blot using the following antibodies: Mouse anti-FLAG M2 (Sigma, 1:10,000). RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... ovaries were incubated overnight at 4°C under agitation with the primary antibody (anti-pTyr (4G10) (05-321, Millipore Sigma, 1:40), anti-pERM (3141S ...
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bioRxiv - Microbiology 2022Quote: ... Each strain to be tested was then washed twice with sterile 1x PBS and adjusted to a concentration of ~2×105 cells/mL in macrophage growth media containing 1 μg/mL of the anti-GXM antibody 18B7 (Sigma Cat# MABF2069). Cells were opsonized in this media for 1h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore, MAB1574, 1:5,000).
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bioRxiv - Neuroscience 2022Quote: ... Larvae were washed three times in 0.1% PBST and incubated with secondary antibodies: anti-mouse Alexa Flour 647, 1:100 (Invitrogen, #28181. Stained larvae were mounted using DAPI Fluoroshield (Sigma-Aldrich, #F6182). Images were collected on a Nikon A1 eclipse Ti confocal microscope.
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bioRxiv - Molecular Biology 2023Quote: ... ChIP experiments were performed basically in accordance with the above procedure using 1 µg of an anti-H4K16ac antibody (Millipore, 07-329) and 2.4 µg of an anti-H2A.Z antibody (Kudo et al ...
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bioRxiv - Neuroscience 2022Quote: ... mid-sagittal cerebellar vibratome sections were blocked and permeabilized with 10% normal goat serum in PBS containing 0.2% Triton X-100 and immunostained with a mouse anti-Calbindin D28k antibody (Sigma-Aldrich, 1:1000) followed by incubation with Alexa Fluor 555-labeled goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution: anti-smooth muscle actin (SMA) 1:500 (Sigma, A2547), anti-GFP 1:200 (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated overnight at 4°C with mouse anti-TH antibody (1:8000 dilution, T2928, Sigma-Aldrich, St. Louise, MO, USA), 1% NHS ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and developed by detecting bound IgG or IgA with appropriate fluorochrome-labelled secondary antibodies: 1 µg/ml goat anti-human IgG-Cy3 (Sigma, Cat# C2571) or goat anti-human IgA-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... The membranes that were loaded with fetal membrane and uterine tissue lysates were re-probed for 1 h at room temperature with a mouse anti-β-actin (ACTB) monoclonal antibody (Cat# A5441, Sigma-Aldrich). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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Characterization of pediatric porcine pulmonary valves as a model for tissue engineered heart valvesbioRxiv - Bioengineering 2023Quote: ... The co-stain dye was prepared from a 1:50 dilution of anti-α-SMA CY3 conjugated primary antibody (Sigma, cat #C6198) and 1:50 dilution of anti-vimentin Alexa Fluor™ 488 conjugated primary antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 16-h incubation in PBS with 0.1% Triton™ X-100 with the following antibodies: mouse monoclonal anti-NeuN (1:500; EMD Millipore, MAB377), rabbit polyclonal anti-KCC2 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then washed and stained with 1:100 dilution of mouse anti-sarcomeric α-actinin primary antibody (A7811, Sigma-Aldrich) and 1:100 rabbit anti-CD31 (AB32457 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with bovine serum albumin (0.5%) before labeling with anti-STIM1 monoclonal antibody (1:50 dilution; 30 min; Sigma-Aldrich, Rockford, IL). Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...