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Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for Mouse Anti Hepatitis C Virus NS5a Antibody 1877 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated at 37ºC for 1 h with Duolink® in situ PLA® Anti-rabbit PLUS and Anti-mouse MINUS (Sigma) and later washed with 0,01% PBS-tween ...
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bioRxiv - Cell Biology 2019Quote: ... K124 and K42 immunoblots were reprobed with anti-keratin K14 and mouse anti-β-actin-HRP mAb (K124 blots; 1:15,000, clone AC-15; Sigma-Aldrich) or anti-β-actin-HRP alone (K42 blots ...
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bioRxiv - Neuroscience 2020Quote: ... as well as secondary anti-mouse and anti-rabbit (Thermo Fischer Scientific) Membranes were incubated with Immobilon® ECL Ultra Western HRP Substrate (Millipore) for 3 minutes prior to image acquisition ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslip were washed with PBS with 0.25% triton X-100 and followed by incubation with secondary antibodies Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:1000) together with Hoechst 33342 1 µM (14533; Sigma-Aldrich) for nuclei staining during 1h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-GSX2 (1:250 dilution; gift from Kenneth Campbell (Toresson et al., 2000)) mouse anti-Olig2 (1:250 dilution; Millipore (#MABN50), Billerica ...
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bioRxiv - Biophysics 2020Quote: ... A second incubation of 1 hour at room temperature was then performed with goat anti mouse Alexa Fluor 555 and anti-rabbit FITC conjugated (1:400, F7512, Sigma Aldrich). For all the labelling ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with primary antibodies diluted with TBS + 2% BSA + 0.1% Triton X-100 at 1:1000 (human anti-ACA, Geisel School of Medicine at Dartmouth) and 1:4000 (mouse anti-α-tubulin, Sigma) following the standard immunostaining protocol as described above ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were Immunoblotted with incubation of anti-Ahnak (rabbit polyclonal, RU2024, 1:5,000) or Anti-Gapdh (mouse monoclonal, MAB374, Millipore, 1:5,000) primary antibody followed by incubation of horseradish peroxidase-linked goat anti-rabbit or anti-mouse secondary antibody (1 ...
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bioRxiv - Microbiology 2020Quote: ... the bound NoV capsid proteins were detected with mouse anti norovirus GII (1:1000; NativeAntigen) and by adding horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma Aldrich). Horseradish peroxidase activity was detected with a TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was incubated in secondary antibody (Biotinylated horse anti-mouse IgG 1:500, BA-2001, Vector Laboratories, Burlingame, CA; biotinylated donkey anti-goat IgG 1:500, AP180B, Millipore-Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... at room temperature and incubated with anti-neurofilament (Chicken, 1:1000, Developmental Studies Hybridoma Bank, 3A10) and anti-NeuN (Mouse, Millipore, MAB377) primary antibodies in blocking buffer for 48 hrs at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10,000 for 1 h (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were incubated in mouse anti-FasII (1:200, Developmental Studies Hybridoma Bank) and rabbit anti-myc (1:1000, Sigma-Aldrich) diluted in blocking buffer for 16 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002; In-Situ Detection Reagents FarRed, Sigma DUO92013) according to the manufacturer’s in situ fluorescence protocol ...
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bioRxiv - Microbiology 2024Quote: ... in Tris-buffered saline with Tween 20 (TBST) and incubated with 1:5000 rabbit anti-FtsA or mouse anti-FLAG (F1804, Sigma-Aldrich) antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... Developmental Studies Hybridoma Bank) primary antibody to detect type I fibers and with anti-laminin antibody (1:200, Sigma) to delineate myofiber outlines present in a muscle section ...
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bioRxiv - Neuroscience 2019Quote: ... cultures were washed three times with medium lacking NGF and then maintained in NGF-deficient media containing a neutralizing antibody (1μg/mL anti-NGF antibody, Millipore) through designated time points at 37°C.
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunoassays: primary antibodies - rabbit polyclonal anti-RTN3 (#ABN1723, Merck Millipore; 1:400 dilution), monoclonal anti-NF200 (#N5389 ...
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bioRxiv - Plant Biology 2022Quote: ... Antibodies used for the western blots were obtained from commercial suppliers: anti-actin monoclonal antibody (Sigma-Aldrich A0480-200μL) and anti-mouse antibody (Agrisera AS111772) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated in 1:50 dilution of primary antibody (anti-lucifer yellow, RRID:AB_2536190) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C under gentle agitation ...
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bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used both anti-α-tubulin antibody and antibody against FMRFamide (rabbit polyclonal; Millipore, Sigma, Cat# AB15348, RRID: AB_805291). After incubating in a blocking solution of 6% goat serum in PBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used both anti-α-tubulin antibody and antibody against FMRFamide (rabbit polyclonal; Millipore, Sigma, Cat# AB15348, RRID: AB_805291). After incubating in a blocking solution of 6% goat serum in PBS ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies and dilutions were as follows: anti-tyrosine-hydroxylase antibody (TH 1:100, AB152, Millipore Sigma, Burlington, MA), anti-calcitonin gene related peptide antibody (CGRP:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... an anti-GFP polyclonal antibody (Medical & Biological Laboratories) and a rabbit horseradish-peroxidase-linked IgG whole antibody (Sigma-Aldrich) were used as the primary and secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then labelled with primary anti-RNAP-II antibodies (CTD Antibody, clone 8WG16, Sigma-Aldrich 05-952-I) and anti-VP (a gift from Anne-Sophie Gosselin Grenet ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in the primary antibody (anti-Kv3.2 antibody, 1:200, Cat.no. APC-011, alomone lab, Israel; anti-GAD67 antibody, 1:200, Cat.no. MAB5406, Sigma, USA) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: Other primary antibodies used were: Atlas Antibody anti-MYO18A (Cat# HPA021121, RRID:AB_1854250) from Sigma-Aldrich (St. Louis, MO, USA); Proteintech (Rosemont ...
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bioRxiv - Molecular Biology 2023Quote: The following primary antibodies were used in this study: anti-v-Src (OP07) antibody was purchased from Sigma-Aldrich. Anti-phospho-Src family (pY416 ...
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bioRxiv - Microbiology 2023Quote: ... The primary antibodies were followed by respective species-specific secondary antibodies conjugated to HRP (Anti rabbit, 1/20000, Sigma) or (Anti mouse ...
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bioRxiv - Cell Biology 2022Quote: ... They were then blocked in 10% goat serum and incubated with primary antibodies (anti-phosphotyrosine antibody (4G10; EMD Millipore), 1:200 ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody incubation was performed for 30min with 1:10000 (v/v) anti-rabbit IgG-peroxidase antibody (Sigma-Aldrich). Again ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used in this study: rabbit anti-EBF1 antibody (1:1000, AB10523; Millipore, Darmstadt, Germany,), mouse anti-MYO7A antibody (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... membrane were firstly immunoblotted with primary antibody diluted in 2% milk PBS-T (generally 1/1000 except for anti H3 whose dilution used is 1/10000) at 4°C overnight and finally immunobloted with secondary antibody coupled to HRP (Sigma) diluted in 2% milk PBS-T at room-temperature for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300, Millipore, Burlinghton, MA, USA; AB5054) diluted in PBS containing 0.3% Triton X-100 and 1% NDS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The membranes were blocked with TBST supplemented with 10% skim milk for 30 mins at room temperature and further probed with primary antibodies at 4°C overnight including Trim17 (1:500, Sigma); Mcl-1 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with a primary antibody for tyrosine hydroxylase (TH) (rabbit, 1:1000, Millipore #657012) diluted in blocking solution ...
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bioRxiv - Molecular Biology 2019Quote: 50 µg of MRC5 chromatin extracts were incubated overnight at 4° C in 150 μl total volume of IP buffer (see above) with antibody (ACTβ: ab8227 Abcam; NMI: M3567 Sigma) (ChIP ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed cells were stained with the following antibodies (for 3 hrs at 37°C): β-Tubulin (1:100, Sigma-Aldrich), DBC1 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were: α-CENP-C (MBL:1:1000) as a kinetochore marker and α-tubulin (Sigma: 1:500). Samples were incubated in primary antibody solution for 1 hour at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma) were added to each sample for 3.5 hours at 4°C after the overnight incubation with antibody ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4°C overnight with the following primary antibodies in 5% BSA in TBST: puromycin (Sigma, 1:25,000) and vinculin (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed for three times with PBS containing 0.1% Triton X-100 and then incubated with fluorescence-conjugated secondary antibodies at 37 °C for 1 h and stained for DNA with 10 mg/ml DAPI (Sigma). The coverslips were sealed with nail polish ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636 ...
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bioRxiv - Neuroscience 2019Quote: ... for 15 min at room temperature and stained overnight with the primary antibodies at 4 °C (Kinesin heavy chain: Millipore MAB1614 1:50 ...