Labshake search
Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Buffer exchanged the elution to PBS using 30-kDa cut-off Amicon ultrafiltration units.For Fab preparation: incubate 500 μg of concentrated polyclonal IgG samples with papain (Sigma Aldrich P3125) in digestion buffer (100 mM Tris ...
-
bioRxiv - Microbiology 2024Quote: ... Adjustment of lactic acid concentration in cell-free supernatants was performed by dilution of a 2M L-lactate stock (Sigma-Aldrich, Belgium) to 140 mM in GASSN.
-
bioRxiv - Microbiology 2024Quote: ... α-amylase (150 units/mL, Sigma-Aldrich), proteinase K (1 mg/mL ...
-
bioRxiv - Microbiology 2024Quote: ... Natural products and benzaldehyde derivatives were purchased from Sigma Aldrich (MO, USA). V-06-018 was synthesized as previously described (19).
-
bioRxiv - Microbiology 2024Quote: ... and lipase (25 mg/mL, Sigma-Aldrich) were used ...
-
bioRxiv - Immunology 2024Quote: ... 50 μl 3,3′,5,5′-tetramethylbenzidine (TMB; Sigma) was added to each well ...
-
bioRxiv - Microbiology 2024Quote: ... USA) and ampicillin and spectinomycin were purchased from Sigma Aldrich (MO, USA). DMSO (solvent for inhibitor compounds) ...
-
bioRxiv - Immunology 2024Quote: Tamoxifen (Sigma-Aldrich, cat # T5648) was dissolved in corn oil at a concentration of 50 mg/mL ...
-
bioRxiv - Immunology 2024Quote: ... Sialic acids were quantified by comparing with known amount of standard mixture (Neu5Gc and Neu5Ac) purchased from Sigma-Aldrich.
-
bioRxiv - Immunology 2024Quote: ... solution (12,5 mg/mL) was prepared in DMSO (Sigma-Aldrich cat # D2650) and stored at 4°C until diluted 10-fold in corn oil just before administration ...
-
bioRxiv - Immunology 2024Quote: ... Transcription was then blocked by incubating the cells with 10 μg/ml Actinomycin D (Sigma). Cells were harvested at different time points (15 ...
-
bioRxiv - Immunology 2024Quote: ... while cell pellets were lysed in 0.5% Triton X-100 (Sigma) in Tyrode’s buffer ...
-
bioRxiv - Immunology 2024Quote: ... injections of LPS (Sigma Aldrich Cat# L2880, 0.1 mg/kg) for ‘priming’ needed to render barrier-housed mice responsive to antibody injections (19) ...
-
bioRxiv - Immunology 2024Quote: ... containing complete Minitab protease inhibitor cocktail (Sigma Alrich, MO, US). Cell homogenates were centrifuged at 800 x g for 3 min at 4°C to remove cellular debris ...
-
bioRxiv - Immunology 2024Quote: ... β-hexosaminidase substrate (4-nitrophenyl N-acetyl-β-D-glucosaminide, 4 mM, Sigma) was then added to the supernatant and lysate for 1 h at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... 1X phosphatase inhibitor (Sigma)) ...
-
bioRxiv - Immunology 2024Quote: ... for 30 min followed by stimulation with 0.2 μg/ml HSA-DNP (Sigma) for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... 1X protease inhibitor (Sigma), 1X phosphatase inhibitor (Sigma)) ...
-
bioRxiv - Immunology 2024Quote: ... Scgb1a1 (Sigma Aldrich Cat# ABS1673) or CD41 (Biolegend Cat# 133939 ...
-
bioRxiv - Immunology 2024Quote: ... together with a FITC-conjugated antibody raised against Acta2 (Sigma-Aldrich Cat# F3777) were incubated overnight at 1:500 with 5% normal donkey serum ...
-
bioRxiv - Immunology 2024Quote: BMMCs were treated with 1 μg/ml IgE–anti-DNP (Sigma) for 30 min followed by stimulation with 0.2 μg/ml HSA-DNP (Sigma ...
-
bioRxiv - Immunology 2024Quote: ... Mouse IgG (Sigma) or rat IgG (Thermo Fisher Scientific ...
-
Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... 100 µg/ml deoxyribonuclease (DNase) I (Sigma-Aldrich) and one of the following enzyme solutions ...
-
Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: Treg cell-depletion was achieved by intraperitoneal (IP) injection 500 ng diphtheria toxin (DT) (Merck-Millipore) dissolved in of 150 µl of PBS on days 0 and 1 ...
-
Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... and 200U/ml DNase I (Sigma, DN25-100MG) for 20 min at 37°C before mechanical disruption using a gentleMACS Octo dissociator ...
-
bioRxiv - Immunology 2024Quote: ... Carbachol (Sigma-Aldrich, 212385); MG624 Cayman Chemical Company ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ CD45.2+ population was collected directly into 500µl Trizol (Sigma) and stored at −80°C until used.
-
bioRxiv - Immunology 2024Quote: ... powder form was carefully dissolved in DMSO (Sigma-Aldrich, D2438) - sterile ...
-
bioRxiv - Immunology 2024Quote: ... washed 3 times with PBS and blocked with 5% bovine serum albumin (Sigma Aldrich, A9647) in PBS for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... fixation step was performed using 4% paraformaldehyde (PFA) (Sigma Aldrich, 158127) and cells were incubated with Hoechst stain (Anaspec ...
-
bioRxiv - Immunology 2024Quote: ... enterica serotype Typhimurium (Sigma-Aldrich, L6143-1 MG); Torin-1 (Tocris ...
-
bioRxiv - Immunology 2024Quote: ... and deoxyribonuclease (DNase) I (20 μg/ml, Sigma-Aldrich cat # DN25). After digestion ...
-
bioRxiv - Immunology 2024Quote: ... sialic acid (500 ng/mL, catalog no. A0812, Sigma Aldrich), or mock-treated ...
-
bioRxiv - Immunology 2024Quote: ... Mice were immunized intraperitoneally with 10 μg of DECA-PA63 or DECA-CRM197 in complete Freund’s adjuvant (Sigma, St. Louis, MO). Mice were boosted at 14 days interval or as required with 10 μg of each conjugate in incomplete Freund’s adjuvant.9 Mice were bled by retro-orbital bleeding using heparin capillary tubes under isoflurane anaesthesia ...
-
bioRxiv - Immunology 2024Quote: ... and then incubated for an additional 3 h with phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) PMA (25 nM ...
-
bioRxiv - Immunology 2024Quote: ... avidin conjugated with alkaline phosphatase (Sigma-Aldrich) was added ...
-
bioRxiv - Immunology 2024Quote: ... cells were lysed in lysis buffer with protease and phosphatase inhibitors (Sigma-Aldrich). Protein concentration in samples was quantified by BCA assay (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... femurs of chickens were removed and placed into 10 cm petri dishes containing PBS with 1% antibiotics (Sigma-Aldrich, St. Louis, MO). Both ends of the femur bone were cut across the tops with sterile bone-scissors and a sterile iron wire was passed through and the bone marrow was flushed with sterile PBS using 20 ml syringe with 16G needle ...
-
bioRxiv - Immunology 2024Quote: ... These two primary immune responses were fate-mapped by oral gavage of 200 µl tamoxifen (Sigma) dissolved in corn oil at 50 mg/ml ...
-
bioRxiv - Immunology 2024Quote: ... Cell suspensions were overlaid with an equal volume of Histopaque 1119 (Sigma-Aldrich, St. Louis, MO) and centrifuged at 1200 g for 30 min at RT to remove red blood cells ...
-
bioRxiv - Immunology 2024Quote: ... and Rotenone (1 μM; Sigma-Aldrich)/ Antimycin A (1 μM ...
-
bioRxiv - Immunology 2024Quote: ... and 1.25 µL of 0.1 M DTT (Sigma-Aldrich). All wells were capped ...
-
bioRxiv - Immunology 2024Quote: Affinity purified His-tagged RBD produced by Expi293 transient transfection was diluted in 0.1 M NaHCO3 pH 9.8 (20 µL of a 2.5 µg/mL solution) and coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... 20 μg/mL gentamicin (Sigma) and 0.2mM hypoxanthine (C-C Pro ...
-
bioRxiv - Immunology 2024Quote: ... samples were incubated with 3,3′,5,5′-Tetramethylbenzidine substrate (slow kinetic form, Sigma) and the reaction was stopped with 1N HCl ...
-
bioRxiv - Immunology 2024Quote: ... Sigma-Aldrich)/ Antimycin A (1 μM; Sigma-Aldrich) in Mitostress assay.
-
bioRxiv - Immunology 2024Quote: ... FCCP (1.5 μM; Sigma-Aldrich), and Rotenone (1 μM ...
-
bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS-Tween (PBS + 0.05% Tween20; Sigma) and blocked with 2.5% BSA in PBS for 2 hours at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... OCR was measured in the presence of Oligomycin (1.5 μM; Sigma-Aldrich), FCCP (1.5 μM ...
-
bioRxiv - Immunology 2024Quote: ... Reaction mixtures were concentrated down to between 0.5-1 mL volume using Amicon 4 mL 10 kDa concentrators (Millipore) then dialyzed using Slide-a-Lyzer MINI 10 kDa dialysis devices (ThermoFisher ...