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Citations for Millipore Sigma :
951 - 1000 of 7712 citations for West Nile Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... proteins were transferred to PVDF (Millipore, IPVH00010) or Nitrocellulose (Sigma_Aldrich ...
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Cancer Biology 2021Quote: Protein extracts were separated from each cell line with RIPA protein lysis buffer (R0278, Sigma, St Louis, MO, USA) containing 1X protease cocktail (P8340 ...
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bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of protein from the different S1 fractions was first treated with PureProteome Protein G Magnetics Beads (Millipore) for contaminant IgG depletion and then directly applied to dry nitrocellulose ...
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bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Developmental Biology 2019Quote: ... The HDAC6 protein was immunoprecipitated from total protein lysates (0.25 mg) of cells treated with different compounds using protein A agarose beads (Sigma). The HDAC6 protein bound beads were used as enzyme source and was incubated with the HDAC assay buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and protein diluted to 1mg/ml 1ml of protein lysate was precleared with 20μl of protein G sepharose bead slurry (Sigma) for 1 hour to remove native immunoglobulins ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein was estimated using the bicinchoninic acid protein estimation method (BCA, B9643, Sigma-Aldrich). We separated a protein sample (80µg ...
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bioRxiv - Paleontology 2023Quote: Information for the protein standards purchased for the ToF-SIMS analyses are as follows: type-1 collagen protein (Sigma, CAS ...
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bioRxiv - Cell Biology 2024Quote: ... Yeast protein extracts were separated on protein gels containing 10% polyacrylamide and transferred to an Immobilon-P membrane (Millipore). The membranes were probed with anti-Myc antibodies (9E10 ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G and Protein A Dynabeads (1:1 mixed) were blocked with 1mg/ml denatured yeast tRNA (Sigma-Aldrich, R5636) and 1mg/ml of BSA (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Immunology 2021Quote: ... Proteins smaller than 30 kDa were removed by passing the protein lysate through an Amicon Ultra - 15 Centrifugal Filter (Millipore). One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Molecular Biology 2019Quote: Purified proteins were concentrated to the indicated protein concentration described in the text using Amicon Ultra centrifugal filters (10K MWCO, Millipore) and added to solutions at varying concentrations with the indic ated final salt and molecular crowder concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... Bcl2/Adenovirus E1B 19 kDa and protein-interacting protein 3-like (BNIP3L/NIX) from Sigma-Aldrich (St. Louis, MO, USA), perilipin (PLIN ...
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bioRxiv - Cell Biology 2020Quote: ... The target protein was detected by specific primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated antibodies (for less abundant proteins) (Sigma) or fluorescence-labeled secondary antibodies (for abundant antigens ...
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bioRxiv - Cell Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore; endogenous RNA pol II IPs) or GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 40μg of total protein were loaded per well in SDS-acrylamide/bisacrylamide gel and proteins were electrophoretically transferred to a PVDF membrane (Millipore). For treatment with MG132 and ABA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed for target proteins with specific antibodies against phosphorylated or total proteins and secondary antibodies before being developed using Immobilon chemiluminescent substrate (Millipore) and visualized using the Odyssey Infrared Imaging System (LI-COR).
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Physiology 2023Quote: ... All images were normalized total protein from the same membrane stained using Amido Black total protein-stained membrane (A8181, Sigma).
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of clear lysates and debris were loaded onto the same 10% Bis-Tris gel for the comparison of total protein (Coomassie staining) and ubiquitinated protein levels (anti-ubiquitin antibody, Sigma, 05-944 ...
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bioRxiv - Cell Biology 2023Quote: ... Instrument performance was monitored daily using a system suitability test data consisting of a 30-minute gradient injection of 30fm BSA and 60fm of casein protein digest (both standards were trypsin digested in-house from commercial protein stocks (Sigma)) that was run before each sample ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 µL was taken as protein input while the remaining lysate was pre-cleared using 100 µL or Protein G Sepharose beads (Sigma) at 4°C for 30 min ...