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Citations for Millipore Sigma :
951 - 1000 of 2557 citations for SOX8 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and proteasome was eluted by adding 3x FLAG peptide (400 μg/ml) (Sigma) in Buffer A”.
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bioRxiv - Systems Biology 2022Quote: ... beads were immersed in elution buffer (0.15 ug/mL 3X Flag peptide (Sigma)) and agitated on rotisserie for 1 hour prior to elution ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide stock solutions were diluted with washing buffer (0.1% NP40 substitute (Sigma 74385) in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... The fractions containing the peptides were pooled and concentrated with an Amicon (Millipore) centrifugal concentrator ...
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bioRxiv - Microbiology 2019Quote: Peptide hydrolysis was assayed using the Cbz-Gly-Gly-Leu-AMC (Millipore Sigma) substrate ...
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bioRxiv - Genetics 2019Quote: ... Chromatin was then eluted from the beads by competition with FLAG peptide (Sigma) and cleavage by TEV protease (New England Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... and eluted with 0.2 mg/mL 3X FLAG peptide (Sigma-Aldrich Cat #F4799). We note that similar cyclin-Cdk fusions have previously been able to restore wild-type function in vivo(23) ...
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bioRxiv - Systems Biology 2021Quote: ... peptides were acidified with formic acid and desalted using C18 Zip-Tips (Millipore) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2020Quote: ... Peptides were reconstituted in high grade DMSO (Sigma-Aldrich, St-Louis, MO, USA) at a concentration of 0.1 mg/ml and used at a final concentration of 0.25 μg/ml in a maximum of 0.2% DMSO ...
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bioRxiv - Immunology 2020Quote: ... The labelled peptide pools were desalted using P10 C18 pipette ZipTips (Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The immune precipitates were eluted by incubation with FLAG peptide (F4799) (Sigma-Aldrich) at room temperature for 15 min and were subjected to silver staining by using SilverXpress (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting peptide mixture was subjected to C18 Zip Tip clean-up (Millipore) before being analyzed by high-resolution LC-MS/MS using an Ultimate 3000 Nano Ultra High Pressure Chromatography (UPLC ...
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bioRxiv - Biochemistry 2021Quote: ... beads were incubated in TBS with 200-ng/μl FLAG peptide (Sigma Aldrich) at 4°C for 30 min.
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bioRxiv - Biophysics 2021Quote: Each lyophilized peptide was dissolved in 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP; Sigma©) at a concentration of 1 mg/mL ...
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bioRxiv - Immunology 2020Quote: ... HLA-peptide complexes were loaded on preconditioned 50mg C18 desalting columns (Sigma Millipore), then followed three 0.1% TFA in water washes ...
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bioRxiv - Immunology 2020Quote: ... Peptides were purified by ultrafiltration using centrifugal filter units (Amicon; Millipore, Billerica, MA), desalted using ZipTip C18 pipette tips (Millipore ...
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bioRxiv - Immunology 2020Quote: ... HLA-peptide complexes were loaded on preconditioned 50mg C18 desalting columns (Sigma Millipore), then followed three 0.1% TFA in water washes ...
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bioRxiv - Immunology 2020Quote: ... The tetramerized peptide–MHC complexes were incubated with 10μM of free biotin (SIGMA) to saturate remaining unbound streptavidins ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were identified by immunolabeling using an anti-myc antibody (C3956, Sigma-Aldrich). Cy2 or Cy3 conjugated donkey secondary antibodies against mouse or rabbit IgG were from Jackson ImmunoResearch (1/300).
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bioRxiv - Microbiology 2022Quote: ... The resulting peptides were desalted with C18 Zip-tip (Millipore, Bedford, MA, USA) for LC-MS/MS analysis.
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bioRxiv - Biochemistry 2022Quote: ... Dried peptides were dissolved in 60 µL of 0.1% trifluoroacetic acid (TFA, Sigma), and desalted using 2-core MCX stage tips (3M ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were eluted with 75 µL of 65% ACN with 5% NH4OH (Sigma), and dried.
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bioRxiv - Biochemistry 2022Quote: ... RAF protein was then eluted with 200 μg/mL 3xFLAG peptide (Sigma-Aldrich) in TN buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were desalted and resuspended in 0.1% FA using C18 stage tips (Millipore).
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bioRxiv - Bioengineering 2024Quote: ... Digested peptides were then desalted using ZipTip according to the manufacturers protocol (Millipore). Desalted peptides were then eluted ...
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bioRxiv - Neuroscience 2024Quote: ... we pre-incubated this primary antibody with the GnRH1 peptide (L7134, Sigma-Aldrich) at increasing concentrations (1 ...
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bioRxiv - Cell Biology 2023Quote: ... and the excess peptide was depleted via Amicon Ultra Centrifugal Filters (Merck Millipore). To label the azide-coupled nanobody with a fluorophore ...
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bioRxiv - Immunology 2024Quote: ... Peptides were desalted and resuspended in 0.1% FA using C18 stage tips (Millipore). Samples were analyzed in a timsTOF Pro with PASEF (Bruker Daltonics ...
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bioRxiv - Biophysics 2024Quote: ... and eluted with elution buffer containing 3X FLAG peptide (10 µg/ml, SIGMA) using gravity flow columns ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of tryptic peptides were loaded onto an Ascentis® (Sigma–Aldrich) ES-C18 column (2.1 mm × 100 mm ...
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bioRxiv - Microbiology 2023Quote: ... Bound polypeptides were eluted stepwise with 250 μg/ml FLAG peptide (Sigma Aldrich) diluted in BC100 buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Elution buffer was prepared immediately before use by adding 3XFLAG peptide (Sigma F3290) to TBS to a final concentration of 150 ng/µL ...
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bioRxiv - Biochemistry 2023Quote: Sequences encoding for these peptides were cloned into the pET-41b vector (Novagen) using the restriction sites MfeI and SalI using standard methods and verified by sequencing.
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bioRxiv - Neuroscience 2023Quote: ... after which the interesting proteins were competitively eluted using 3XFLAG peptide (Sigma, F4799). The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... the peptides were cleaned using C18 ZipTip® (Merck Millipore, Massachusetts, United States). Samples were run on a Bruker timsTOF Pro mass spectrometer (Bruker Daltonics ...
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bioRxiv - Neuroscience 2023Quote: ... The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore) and resuspended in 0.1% formic acid in HPLC water.
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bioRxiv - Immunology 2023Quote: PBS or HY peptides were emulsified in Complete Freund’s Adjuvant (Sigma, Lyon, France) at a 1:1 ratio ...
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bioRxiv - Biochemistry 2023Quote: All peptides were manually synthesized on Rink-amide ChemMatrix® resin (Sigma-Aldrich) using DIC/oxyma mediated coupling of 5x molar excess of common Fmoc-protected amino acids for one hour or 2x molar excess of Fmoc-Lys(Boc ...
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bioRxiv - Immunology 2023Quote: ... and precipitated proteins competitively eluted with Flag peptide (150 ng/µl; F4799, Sigma). Lysates were denatured in SDS-PAGE buffer (4x Lämmli buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides formed from the digestion were further purified by C18 ZipTips (Millipore) and analyzed by LC-MS/MS ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were desalted and resuspended in 0.1% FA using C18 stage tips (Millipore). Digested samples (500 ng ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of tryptic peptides were loaded onto an Ascentis® (Sigma–Aldrich) ES-C18 column (2.1 mm × 100 mm ...
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bioRxiv - Molecular Biology 2023Quote: ... Elution was performed by incubation of the beads with 3xFLAG peptide (Sigma Aldrich) at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and nucleosomes were eluted by incubating the beads with 3xFLAG peptide (Sigma Millipore) at a concentration of 500 ng/μL in Buffer D for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... and nucleosomes were eluted by incubating the beads with 3xFLAG peptide (Sigma Millipore) at a concentration of 500 ng/μL in Buffer D for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were eluted with ABC buffer and then desalted with C18 tips (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound complexes were eluted by incubation with 500 µg/mL 3xFLAG peptide (Sigma) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For the tandem IP experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For the tandem IP experiment ...