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Citations for Millipore Sigma :
951 - 1000 of 7537 citations for Recombinant NRG4 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Endogenous HA-FLAG-tagged PPS parasite samples were probed with Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and donkey-anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
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bioRxiv - Biophysics 2020Quote: HP1α-AID-sfGFP cells expressing mCherry-tagged HP1αWT or HP1αI165E were treated with control media (no auxin) or 1 mM auxin (NaIAA, Sigma #I5148) for 16 hours before being fixed in 4% paraformaldehyde for 10 minutes ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Microbiology 2023Quote: ... and analyzed by Western blotting for detection of 3xFLAG-tagged IsdF using an anti-FLAG M2 antibody (Sigma, #F3165) and infrared imaging (Odyssey CLx ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
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bioRxiv - Genetics 2023Quote: Human mCherry-tagged CTNS mRNA solutions were prepared in RNAse free water (500ng/µl) with 0.05% PhenolRed (Sigma, cat.no.P0290). All mRNA injections were performed at the one-cell stage using a FemtoJet microinjector (Thermofisher ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... The FLAG-tagged RIG-I was pulled down using anti-FLAG M2 magnetic beads purchased from Sigma-Aldrich (M8823). The proteins were eluted from beads in 30 μL of 4x Laemmli buffer heated to 95 °C for 5 mins and the entire elution was run on a Western blot using anti-FLAG (Sigma-Aldrich F3165 ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Microbiology 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated using a 30K MWCO concentrator (EMD Millipore) to 0.2 ml and diluted to 0.5 ml in GF150 buffer (25 mM HEPES [pH7.4] ...
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bioRxiv - Biochemistry 2024Quote: ... which contained soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... which containing soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the bound (B) and unbound (UB)/flow through were analyzed by Western blotting using anti-GST rabbit polyclonal antibody (Sigma #G7781, 1:5000 dilution).
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bioRxiv - Cell Biology 2019Quote: ... and proteins precipitated using protein-A Agarose (Sigma-Aldrich) prior to analyses by Western blotting.
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bioRxiv - Microbiology 2022Quote: ... proteins proteins were electrophoretically transferred to nitrocellulose membranes (Millipore) using a Transblot semi-dry transfer cell (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Biochemistry 2019Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Samples of episomal PPS-GFP Dd2 or endogenously HA-FLAG-tagged PPS Dd2 parasites were harvested by centrifugation and treated with 0.05% saponin (Sigma 84510) in PBS for 5 min at room temperature and spun down by centrifuge at 5,000 rpm for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were hybridized overnight at 37°C with sixteen FLAP-Y-Cy-3 tagged complementary oligonucleotide probes, targeting regions in Cox7c mRNA (IDT) in Hybridization mix (15 % Formamide, 1.7 % tRNA (Sigma; R1753), 2 % FLAP:Probe mix ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells plated in six well plates were transfected with 1.8□μg of C-terminally tagged histone H3-HaloTag Fusion Vector DNA and 0.2□μg of C-terminally tagged NSD2-PWWP1-NanoLuc (Wt or F266A) or NSD3-PWW1-NanoLuc using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2022Quote: ... The purification of 3XFLAG-tagged 26S proteasome was carried out by affinity purification using M2 anti-FLAG beads (Sigma A2220). After incubation for 1.5 hrs at 4°C the proteasome was eluted with FLAG peptide ...
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bioRxiv - Genetics 2022Quote: ... Degradation of the AID-tagged RAD21 was induced with addition of 500 μM indole-3-acetic acid (auxin; Sigma-Aldrich). Degradation of AID-tagged WAPL was induced with addition of 1μM 5-Ph-IAA (modified auxin ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Molecular Biology 2021Quote: FLAG-tagged RPL3 was transiently expressed in METTL18 KO cells and immunopurified with ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich). The purified proteins were incubated in 1× reaction buffer (50 mM Tris-HCl pH 8.5 and 50 mM MgCl2 ...
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bioRxiv - Genetics 2020Quote: ... The expression of tagged MCM2 in HeLa and Patski cells was confirmed by Western blot using anti-MCM2 rabbit polyclonal antibody (HPA031495 Sigma) at 1:1,000 dilution.
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...