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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and alkaline phosphatase (AP) staining was performed using the Leukocyte Alkaline Phosphatase kit (Sigma-Aldrich). Alternatively ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% SDS) supplemented with both phosphatase and protease inhibitors (phosphatase inhibitor cocktail (PIC, Sigma #P2850), 1 mM sodium orthovanadate (Na3VO4 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% SDS) supplemented with both phosphatase and protease inhibitors (phosphatase inhibitor cocktail (PIC, Sigma #P2850), 1 mM sodium orthovanadate (Na 3VO4 ...
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bioRxiv - Cell Biology 2023Quote: Alkaline phosphatase test was performed using the Leukocyte Alkaline Phosphatase Kit (86R-1KT, Sigma-Aldrich). First ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies were bound to protein G-Sepharose (Sigma-Aldrich) in RSB-100 buffer containing 0.1% NP40 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit anti-Protein A antibodies (Sigma Cat. No P3775) was used for ChIP at a final concentration of 20 μg/ml of immunoprecipitate (IP) ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-green fluorescent protein antibody (Sigma, SAB4301138). In most cases ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were probed with antibodies against FLAG M2 (Sigma), Cdc2 (Santa Cruz Biotechnologies SC-53217) ...
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bioRxiv - Physiology 2023Quote: ... Actin protein was probed (monoclonal antibody from Sigma-Aldrich) as a control to verify equivalent protein loading between the variants ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2, Millipore Sigma #04-263) at a dilution of 1:400 for 3 hours at room temperature.
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Microbiology 2021Quote: ... MERS S-protein was detected using a monoclonal anti-FLAG M2-HRP conjugated antibody (SIGMA, A8592 @ 1:2000) which bound to a C-terminal FLAG-tag ...
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bioRxiv - Physiology 2020Quote: ... and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000, Sigma, #S8809), the anti-transferrin receptor mouse monoclonal antibody (TransR ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then analyzed by western blotting using a primary anti-biotin antibody (1:5,000 dilution; Sigma-Aldrich) and an anti-mouse secondary antibody coupled to peroxidase (1:10,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to PVDF membranes and probed with M2 anti-FLAG antibody (Sigma; 1 in 5,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Neuroscience 2022Quote: ... and immunoblotted for protein expression using the following antibodies: guinea pig anti-GLT1 at 1:25,000 (Millipore AB1783) and mouse anti-beta actin at 1:20,000 (Sigma-Aldrich A1978) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to PVDF membrane and probed with M2 anti-FLAG antibody (Sigma; 1 in 1,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Cell Biology 2020Quote: Antibodies against the following proteins were used at indicated dilutions: anti-acTub (Sigma-Aldrich T7451, mouse 1:2,000), anti-Arl13b (Proteintech 17711-1-AP ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... the primary antibody was glial fibrillary acidic protein (GFAP) (1:1000 dilution; Millipore, Temecula, CA, USA; Cat. #MAB360). Secondary antibodies were conjugated to Alexa Fluor 594 (donkey anti-mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Bioengineering 2020Quote: ... The mouse anti-HA antibody (Covance, Princeton, NJ, USA) and goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich) were added to the wells for incubation successively ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then washed three times with TBS-T and incubated with alkaline phosphatase-conjugated secondary antibodies (Sigma) in 5% milk in TBS-T for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Color reaction was performed after incubation with anti-DIG antibody conjugated to alkaline phosphatase (Sigma-Aldrich Cat#11093274910) using NBT/BCIP substrate (Roche Cat#11697471001) ...
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bioRxiv - Immunology 2023Quote: ... and plates were probed with IgG1-and IgG2a-specific secondary alkaline phosphatase (AP)-conjugated detection antibodies (Sigma-Aldrich #SAB3701172 and #SAB3701179 ...
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bioRxiv - Cell Biology 2022Quote: Knockdown of DYNC1H1 subunit of dynein was achieved by using mission® esiRNA from Sigma (EHU059651). The esiRNA was prepared according to the manufacturer’s instructions and the cells were transfected with the esiRNA pool with Hiperfect (Qiagen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The 50S subunit sample was then concentrated using a 100 kDa cut-off spin filter (Millipore) and washed with buffer A-1 ...
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bioRxiv - Systems Biology 2019Quote: ... we harvested cells at indicated time points and isolated proteins by lysis in the presence of protease and phosphatase inhibitors (Roth and Sigma-Aldrich), Trichostatin A (APExBio ...
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bioRxiv - Neuroscience 2022Quote: Each hemibrain was weighed prior to homogenizing in tissue protein extraction reagent (T-PER ThermoScientific; 1mL per 100mg of tissue weight) supplemented with protease and phosphatase inhibitors cocktail (Sigma-Aldrich). Total protein concentration was measured using bicinchoninic acid (BCA ...
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bioRxiv - Cancer Biology 2022Quote: ... purified the protein using Ni- resin and exchanged the bound nucleotide for non-hydrolysable GTP analogue GMPPCP using alkaline phosphatase beads (Sigma-Aldrich) and following the protocol of John et.al ...
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bioRxiv - Physiology 2023Quote: ... For protein extraction the tissue was homogenised in RIPA buffer with a cocktail of phosphatase inhibitors (Sigma-Aldrich, Merck, Gillingham, Dorset), using a sonicator three times for 30s each time ...
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bioRxiv - Biochemistry 2019Quote: ... 0.6 µM GTP-gamma-S in the presence of 60 Units of Thrombin (Sigma-Aldrich T1063-1KU). The sample was then passed through a Ni-agarose column (3 ml ...
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bioRxiv - Cancer Biology 2023Quote: ... gamma-h2AX levels were measured for conditions indicated in figures using manufacturer’s protocol (Sigma, no. 17-344). 200k cells were used per replicate per condition and assay was run in technical triplicate per condition.
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Physiology 2021Quote: ... 500 μg of lysates/mouse were then immunoprecipitated with anti-PKG antibody(19) and protein conjugated-Sepharose beads and blotted with anti-NT antibody (Millipore, Cat#05-233, 1:2000). The same membrane was also blotted with anti-PKG antibody as a loading control ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for immunofluorescence (IF) in this study: a monoclonal antibody (mAb) against tau protein (clone Tau-1; Millipore, Cat Number: MAB3420, RRID: AB_94855) diluted 1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.01% SDS supplemented with protease inhibitor and phosphatase inhibitor (1:10 11836170001, Sigma-Aldrich, 1:100 P5726, Sigma Aldrich) was added to the sample and incubated for at least 30 min with 25 μl Dynabeads A (10001D ...
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bioRxiv - Cell Biology 2022Quote: ... 0.01% SDS supplemented with protease inhibitor and phosphatase inhibitor (1:10 11836170001, Sigma-Aldrich, 1:100 P5726, Sigma Aldrich) was added to the sample and incubated for at least 30 min with 25 μl Dynabeads A (10001D ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1% Phosphatase inhibitors cocktail 2 Sigma-Aldrich Cat:P5726 ...
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bioRxiv - Pathology 2024Quote: ... supplemented with phosphatase and protease inhibitor (539132, Millipore, 1:100; P0044; Sigma-Aldrich, St. Louis, MO, USA; 1:100). Before clarification ...