Labshake search
Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM, Sigma) was dissolved in DMSO at 4 mg/ml as stock solution and diluted 20 times using a buffer containing 25 mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: ... and Heptakis(2,6-di-O-methyl)-β-cyclodextrin (Sigma-Aldrich, 39915-1G). The assays were measured at different time points (2d ...
-
bioRxiv - Cell Biology 2023Quote: ... were treated for 45 min with 1.5% methyl-β-cyclodextrin (MβCD, Sigma) in DMEM/F-12 to deplete cellular cholesterol ...
-
bioRxiv - Biophysics 2023Quote: ... the master was developed in propylene glycol methyl ether acetate (Sigma-Aldrich) to remove any photoresist which had not cross-linked.
-
bioRxiv - Cancer Biology 2023Quote: ... Antibodies used: Asymmetric Di-Methyl Arginine ASYM25 (Sigma, #09-814, 1:1000), Tubulin (Santa Cruz #sc-5286 ...
-
bioRxiv - Physiology 2023Quote: A solution of 10 μM palmitoyl-L-carnitine-(N-methyl-d3) (Sigma), 10 μM palmitic acid-d31 (Sigma) ...
-
bioRxiv - Biophysics 2023Quote: ... 1 μl of 200 mM methyl viologen dichloride hydrate (856177, Sigma-Aldrich) and 75 mM MgCl2.
-
bioRxiv - Cell Biology 2023Quote: ... and methyl jasmonate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Prostaglandin E2 was purchased from Tocris (Abingdon ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... NaCl was replaced with 110 mM N-methyl-D-glucamine (NMDG, Sigma)and 15 mM Tris-base ...
-
bioRxiv - Physiology 2023Quote: ... 1mL of 250mM Paraquat (Methyl viologen dichloride hydrate, 856177-1G, Sigma Aldrich) solution was added to the 60 mm NGM plates and plates were put on a shaker for 1 hour ...
-
bioRxiv - Biophysics 2023Quote: ... the master was developed in propylene glycol methyl ether acetate (Sigma-Aldrich) to remove any photoresist which had not cross-linked ...
-
bioRxiv - Immunology 2023Quote: ... PTC-028 (Sapphire Bioscience) was dissolved in 0.5% Methyl cellulose (Sigma-Aldrich) 1% Tween 80 (MPBio ...
-
bioRxiv - Microbiology 2020Quote: The fluorescent DNA-binding stain DAPI (Sigma Aldrich) was used to visualise cell distribution as described previously (63) ...
-
bioRxiv - Cell Biology 2022Quote: ... OEC-2 was cultured on human fibronectin-coated plates (15 μg/ml; EMD Millipore) in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio ...
-
bioRxiv - Immunology 2021Quote: ... supplemented as per RF10 above with the addition of 2 % human AB serum (Sigma).
-
bioRxiv - Microbiology 2023Quote: ... 10 μg/mL goat anti-human IgG1 Fc F(ab’)2 (Sigma-Aldrich, I3391) was coated in 96-well NUNC Immunoplate (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific L3000015 ...
-
bioRxiv - Cell Biology 2024Quote: ... 2×106 sperm/mL were incubated in human tubular fluid (HTF) medium (EMD Millipore) at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
-
bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were co-expressed in Rosetta 2(DE3)pLysS Ecoli (Novagen). Cells were grown at 37°C in 2XYT media to an OD600 of 0.8 ...
-
bioRxiv - Microbiology 2021Quote: ... Protein extraction was performed in the presence of 2% SDS (Sigma) and 4 M acetic acid (Fisher ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 µg of protein was reduced with 2 mM dithiothreitol (Sigma) for 60 minutes at 55 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt Bis-Tris gels (Thermo Fisher) ...
-
bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
-
bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
-
bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
-
bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
-
bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
-
bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
-
bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
-
bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
-
bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
-
bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
-
bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
-
bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
-
bioRxiv - Cell Biology 2024Quote: ... purified CAPS-GST protein was mixed with increasing concentrations of microtubules in Ca2+ Binding-Buffer (80 mM PIPEs, 2 mM MgCl2, 50 uM CaCl2, 1 mg/ml BSA, 10 uM leupeptin (Sigma Aldrich L-2884), 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;) ...
-
bioRxiv - Neuroscience 2020Quote: ... Flat-mounted retinas were incubated overnight at 4°C with antibodies against RNA-binding protein with multiple splicing (RBPMS; EMD Millipore Corp., Burlington, MA) diluted 1:500 in blocking buffer (2% bovine serum albumin ...
-
bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
-
bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...