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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Developmental Biology 2020Quote: ... 100μL of freshly-thawed pronase E (15mg/mL, Sigma P8811 in egg buffer) was added and larvae incubated for exactly 7 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: Di-E-GSSG was prepared by the reaction of eosin isothiocyanate (Sigma-Aldrich) with L-glutathione oxidised (Sigma-Aldrich Lot#100K72765 ...
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bioRxiv - Immunology 2019Quote: ... E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore) by spinning at 3000g for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... reactions were terminated with 0.1 mM E-64 (Sigma Aldrich, St. Louis, MO), a broad-spectrum cysteine protease inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were coated with lectin (from Phaseolus vulgaris ; PHA-E, Sigma product L8629), washed ...
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bioRxiv - Immunology 2019Quote: ... E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore) by spinning at 3000g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on a Poly-D-Lysine (Millipore Sigma, A-003-E) and ECM coated 8-well chamber slide (Millipore Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... or (iv) pretreated with Vitamin C or E (0.2 mM; 0.01 mM; Sigma) for 24 hours before staurosporine incubation ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... (E)-2-decenal were all obtained from Sigma-Aldrich (St. Louis, Missouri, USA). Dopamine was obtained from Tocris Bioscience (Bristol ...
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bioRxiv - Immunology 2019Quote: ... sections were stained with hematoxylin and eosin (H&E) (Sigma, St. Louis, MO) (Nguyen et al ...
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bioRxiv - Physiology 2023Quote: ... and stained with hematoxylin and eosin (H&E) or Masson’s trichrome (Sigma-Aldrich). To prepare frozen sections ...
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bioRxiv - Microbiology 2022Quote: ... and E-64d used in the viral inhibition assays (208733-1SET, Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... 0.1% BSA) with or without 400 ng/mL Pronase E (Millipore Sigma P8811) as described previously70 ...
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bioRxiv - Cell Biology 2023Quote: Sixteen-well E-plates (Ozyme) were pre-coated with 25µg/ml Fibronectin (Sigma) for NPCs or used uncoated for astrocytes ...
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bioRxiv - Developmental Biology 2023Quote: ... The first series was stained with hematoxylin and eosin (H&E, Sigma Aldrich) for scoring ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µm sections were stained with hematoxylin and eosin (H&E, Sigma-Aldrich) or processed for immunohistochemical analysis ...
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bioRxiv - Microbiology 2023Quote: ... E and/or N in the presence of 5 μg/ml polybrene (Millipore). 1 ml of each undiluted vector preparation was used for transduction of cells plated in one well ...
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bioRxiv - Genomics 2023Quote: Lipopolysaccharide (LPS; E. Coli serotype 00:55 B5 LPS (Sigma Lot nr. 025M4040V)) was diluted to the final concentration (0.02 µg/µl ...
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bioRxiv - Genomics 2023Quote: ... coated with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich A-003-E). CRISPRmap barcode readout and antibody staining were performed as described above in the DDR364-irradiation base-editing screen on MCF7-BE3 cells with minor modifications ...
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bioRxiv - Microbiology 2024Quote: ... chloramphenicol (V. cholerae 3 µg/mL, E. coli 15 µg/mL) (Sigma-Aldrich) and/or gentamicin (10 µg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Thapsigargin ((3S,3aS,4R,6R,7S,8R)-6-acetoxy-4-(butyryloxy)-3,3a-dihydroxy-3,6,9-trimethyl-8-(((Z)-2-methylbut-2-enoyl)oxy)-2-oxo-2,3,3a,4,5,6,6a,7,8,9b-decahydro-1H-cyclopenta[e]azulen-7-yl octanoate) from Sigma was dissolved in DMSO to a stock concentration of 5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with 50ng/mL LPS (E. coli O111:B4, Sigma: L3024) and 30ng/mL recombinant mouse IFNγ (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... which consisted of William’s E medium supplemented with 0.1% Polyvinyl alcohol (Sigma-Aldrich), 0.1 mM nonessential amino acids ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) for 20-24h at 20°C ...
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bioRxiv - Cell Biology 2021Quote: ... eosinophil peroxidase (EPX) and Fibroblast specific protein-1 (FSP-1) were procured from Sigma-Aldrich (Merk). All other important reagents were procured from sigma other than specified Sigma-Aldrich (Merk).
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with primary antibodies diluted with TBS + 2% BSA + 0.1% Triton X-100 at 1:1000 (human anti-ACA, Geisel School of Medicine at Dartmouth) and 1:4000 (mouse anti-α-tubulin, Sigma) following the standard immunostaining protocol as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Human ovarian cancer cell line OV90 (ATCC CRL-11732) was cultured in 1:1 volume of MCDB 105 medium (Sigma-Aldrich) and Medium 199 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and and CD44 (SAB1405590) (mouse) at 1:400 dilution for zebrafish tissue and 1:50 dilution for human tissue (Sigma Aldrich). The following secondary antibodies were used ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary emerin (Protein-tech, cat# 10351-1-AP, 1:3,000 dilution) and γ-tubulin (Sigma, cat# T6557, 1:25,000 dilution) antibodies were incubated overnight with rocking at 4°C and secondary antibodies (goat anti-rabbit [cat# 31462 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with 1 ml of Stimulation Medium supplemented with 0.5 nM human insulin (Sigma) for 5 min ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... or alkaline phosphate-labeled goat-anti-human IgM (1:2000, A9794, Sigma, St. Quentin Fallavier France). Conjugate binding was quantified with SIGMAFAST p-nitrophenyl phosphate (Sigma ...