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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Influenza viruses as well as vesicular stomatitis virus strain Indiana (VSV) were propagated on Madin-Darby canine kidney (MDCKII) cells cultured in minimal essential medium (MEM-Sigma) containing 10% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Virus transduction capability was then tittered on 293T-Ace2 cells treated with 50μl of 5μg/ml polybrene (Sigma-Aldritch LLC). LzGreen titer was determined by fluorescence using BZ-X700 all-in-one fluorescent microscope (Keyence) ...
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bioRxiv - Immunology 2021Quote: ... Dengue virus was added at a MOI=1 in a minimum volume of Dubelcco’s PBS with CaCl2 and antibiotics (SIGMA-Aldrich). After 2 hours ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cells were resuspended at a concentration of 106 cells/mL in virus-containing medium supplemented with 10 μg/mL polybrene (Sigma), divided into 6-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-CAMSAP2 was eluted in 50 μl TEV cleavage buffer containing 0.5 μg of glutathione S-transferase-6x-histidine Tobacco etch virus protease site (Sigma-Aldrich) for 2 hr at 4 °C.
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bioRxiv - Immunology 2022Quote: ... hTert-BJ1 or U937 were infected with 2 ml of fresh virus in presence of 8µg/ml Protamine (Millipore Sigma). To generate transduced MDDCs ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and the virus was resuspended in ultrapure water containing 0.005% Bovine serum albumin (Sigma-Aldrich, USA). Purified and filtrated SARS-CoV-2 pseudovirus was firstly fixed in 2% glutaraldehyde (Leagene ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were infected with either of the sgPLXNB2 clones or the control sgRNA with Cas9-GFP virus (Sigma-Aldrich #0030) at 10 IFU/cell in Opti-MEM (Thermo Fisher Scientific #31985070 ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Neuroscience 2023Quote: ... The media containing lentivirus was collected and virus particles were concentrated 20- fold using Amicon Ultracel-100k (EMD Millipore 901024). For all the experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... 786-O cells were cultured with virus containing media and 4µg/mL polybrene (Sigma Aldrich, Cat. No. TR-1003-G) for 24 hours ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Biophysics 2023Quote: ... The cells were then infected in triplicate with VSV-G-pseudotyped GFP-encoding virus in the presence of polybrene (10 µg/ml; Sigma) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was refreshed the next day and virus-containing medium was collected and concentrated 72 hours post-refreshing using Amicon Ultra 15 filters (C7715, Merck Millipore).
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bioRxiv - Pathology 2024Quote: ... Huh7 infection was repeated and non-internalized virus particles were removed by adding 2 mg/ml of pronase (Sigma-Aldrich) in 200 µl of serum-free medium for 5 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... The 54%-40% fraction containing the virus was collected using an 18-gauge needle and concentrated using an Amicon Ultra Centrifugal Filter Ultracel-100K (Millipore). The titer of each AAV (in vector genomes (VG)/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Fibroblasts were isolated from normal human skin and immortalized using human papilloma virus type oncogenes E6/E7 as previously described66 and cultured at 37 °C and 5% CO2 in DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: Virus lytic replication in TREx-BCBL1-RTA cells was induced via addition of 2 µg/ml doxycycline hyclate (Sigma-Aldrich). HEK-293T-rKSHV.219 cells were induced via addition of 20 ng/ml TPA and 3 mM sodium butyrate ...
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bioRxiv - Microbiology 2024Quote: ... Virus lytic replication in TREx-BCBL1-RTA cells was induced via addition of 2 µg/ml doxycycline hyclate (Sigma-Aldrich)30 ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts were seeded and allowed to attach overnight before incubation with the virus and polybrene (8 µg ml−1, Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK 293T cells seeded on a 6-well plate (pre-treated with poly-L-lysine) were incubated in virus-resuspended media supplemented with 0.4 µg/mL polybrene (Millipore Sigma). The plate was centrifuged at 931 g for 2 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... with each virus (DENV, ZIKV, KUNV) was collected 5–8 days postinfection and concentrated using 100-kDa Amicon filter units (Millipore) by centrifuging at 3250xg for 20 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... HEK-293T cells were transfected with pHW2000 reverse genetics plasmids (250 ng of each plasmid in 6-well plate format) and the media changed to ‘virus growth medium’ (serum-free DMEM supplemented with 1 ug/mL TPCK-trypsin (Sigma) and 0.14% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... OCI-AML3 cells were spinofected with virus particles (60 min at 2000 rpm) in the presence of 5µg/ml polybrene (Sigma Aldrich). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were infected for 16-24 h with virus in the presence of 2 μL polybrene (TR-1003G, Sigma-Aldrich), washed ...
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bioRxiv - Cancer Biology 2024Quote: ... 105 target cells were seeded per well in a 24-well plate for 24 hours prior to viral infection with 0.5 ml of virus suspension in complete medium in presence of 8 μg/ml Polybrene (TR-1003-G, Millipore Sigma).
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bioRxiv - Cell Biology 2024Quote: ... cells were divided into 12-well plates at a density of 2 million cells per along with 75 µL virus and 0.8 µg/mL polybrene (Millipore, TR1003G). Plates were centrifuged at 37 °C for 2 h at 830g ...
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bioRxiv - Cancer Biology 2024Quote: ... CMT167 cells were infected overnight with scrambled or Urod shRNA (2 independent shRNAs) concentrated virus and 8ug/mL polybrene (Sigma). Transduced cells were selected in 2ug/mL puromycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... The virus was added to MIA PaCa-2 Luc+ cells or KPCY Luc+ cells along with 10ug/mL polybrene (Sigma) for 72 hours ...
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bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were cultured in hypoxia and treated with 50µM 3-bromopyruvate (3-BP) (Sigma, 376817) dissolved in DMSO.
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bioRxiv - Immunology 2021Quote: ... (3) TexMACS™ medium (Miltenyi) was supplemented with 3% heat-inactivated human AB serum (Sigma) and 1X Penicillin Streptomycin solution (TAC) ...
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bioRxiv - Plant Biology 2021Quote: ... lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone) were purchased from Sigma-Aldrich. Vismione H and madagascine were obtained from PGE2 fraction of Psorospermum glaberimum as previously described (Gallé ...
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bioRxiv - Molecular Biology 2022Quote: ... home-made LIF conditioned medium and 2i inhibitors: 3 μM GSK-3 inhibitor XVI (Sigma) and 10 μM MEK inhibitor PD0325901 (Tocris)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Developmental Biology 2022Quote: Caspase 3 activity was measured using the Caspase-3 Colorimetric Activity Assay Kit (APT131, Millipore). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... we prepared a mixture containing 100mM 1-Ethyl-3-(3’-dimethylaminopropyl)carbodiimide HCL (8510070025, Sigma), and 200mM N-Hydroxysuccinimide (130672 ...