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Citations for Millipore Sigma :
951 - 1000 of 6722 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 10 μM DTT (Sigma-Aldrich), alkylated with 50 μM iodoacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... suis protein homogenates were proteolysed using trypsin (Sigma-Aldrich, Trypsin Sequencing Grade from bovine pancreas ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to 0.22μm PVDF membrane (Millipore, ISEQ00010) using tank transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was concentrated using Amicon 10K centrifugal filters (Millipore). PMSF was added to all buffers including PBS and DTT was also added to the elution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... Elutions were precipitated using ProteoExtract Protein precipitation kit (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 V for 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore) using 1× Power Blotter 1-Step Transfer Buffer (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... proteins were reduced and alkylated with dithiothreitol (Sigma 43815) and iodoacetamide (Sigma I6125 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a Immobilon PVDF membrane (Millipore), which was then blocked in 4% milk in tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore). Membranes were incubated overnight at 4°C with the specific primary antibodies anti-αSMA and anti-tubulin (Supplementary table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were concentrated using Amicon Ultra-15 (Millipore) tubes before loading to a Superdex 75 column ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were immunoprecipitated using anti-Flag (M2, Sigma), anti-Myc (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ReadyBlueTM protein stain were purchased from Sigma-Aldrich. PUREfrex® 1.0 Solutions II (enzymes) ...
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bioRxiv - Developmental Biology 2023Quote: ... Ezview Red Protein G Affinity Gel (Sigma-Aldrich, E3403) was used for negative control samples ...
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bioRxiv - Genomics 2023Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) at 95°C for 10 min and alkylated for 30 min with 40 mM iodoacetamide (Pierce) ...
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bioRxiv - Immunology 2023Quote: ... filtered through 0.45 μm low- protein-binding filters (Millipore) and concentrated via 16 hours centrifugation at 3000 x g at 10 °C ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
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bioRxiv - Microbiology 2024Quote: ... Protein transfer to a PVDF-FL membrane (Millipore, #05317) was achieved at a current of 2.5 mA/cm2 for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto PVDF membrane (EMD Millipore IPVH00010) for 2.5 hours at 200 mA in ice-cold transfer buffer (25 mM TrisHCl ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich) for 30 min at 37 °C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the BCA kit (Sigma), except for insoluble fractions due to due to formic acid’s denaturation effect ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to PVDF membranes (Merk Millipore) and probed with the appropriate indicated antibodies.
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bioRxiv - Molecular Biology 2024Quote: ... A mixture of Protein A agarose (Millipore, 16-157) and Protein G agarose (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a PVDF membrane (Millipore), and the membrane was blocked with blocking buffer (TBST + 5% non-fat dry milk ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated in a 3 kDa concentrator (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The proteins were transferred to Immobilon-FL (Millipore IPFL00010).
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bioRxiv - Biochemistry 2024Quote: ... proteins were expressed in BL21 (DE3)T1R cells (Sigma) at 18 °C overnight after induction with 1 mM IPTG ...
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bioRxiv - Biophysics 2024Quote: ... the proteins were concentrated using 30kDa MWCO Amicon (Millipore) ultra-centrifugal filters to 10 mg/mL and immediately injected in gel filtration column S200 10/300 (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µL of the Protein G agarose bead (Millipore) slurry was washed and added to the antibody-lysate mixture and incubated for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein G beads (Millipore, Cat. #16-266) to the immuno-complex for 2h ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Genetics 2024Quote: ... Co-purifying proteins were imaged with anti-HA (Sigma) and anti-VSV (Bethyl Laboratories ...
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bioRxiv - Immunology 2024Quote: ... 20 μg of Ovalbumin recombinant protein (Sigma-Aldrich, A5503) (1:1 mixed with Alhydrogel ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by Bradford assay (Sigma, #B6916), after which samples were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Biochemistry 2024Quote: ... protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chlorophorm phase separation ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were blotted onto a nylon membrane (Millipore). Blots were blocked using 5% nonfat dry milk (NFDM ...
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bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysates (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10-μg protein samples were separated on NuPAGE 4%-12% Bis-Tris protein gels and transferred onto Immobilon-P PVDF membranes (EMD Millipore, Billerica, MA). The membranes were incubated for 1 hour in blocking buffer (Tris-buffered saline with 0.1% Tween and 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-HKU1 proteins or lacking the fusion protein (Δ Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland). HAE cultures were washed thrice with 100 μl of 1 × PBS and subsequently inoculated with S-HKU1 or VSV-G pseudoviruses ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysate (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...