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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The crosslinking reaction was performed at final concentrations of 5 mM tyramine (99% Tyramine, Sigma-Aldrich, St. Louis, MO), 10 mM H2O2 (30% Hydrogen Peroxide solution ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampi tissues were manually homogenized in 300 µl ChABC buffer (40 mM Tris-HCl pH 8.0, 40 mM sodium acetate, C3667, Sigma) containing Benzonase supplemented with Halt protease/phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and the suspension was then passed through a 40 μm cell strainer and resuspended in 40% Percoll (Millipore Sigma). The Percoll cell suspension was then overlaid onto 70% Percoll and centrifuged at 939 xg for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (PSD95 1:500, Antibodies Inc., Davis CA and MAP2 1:1000; Millipore) were diluted in 1x PBS with 1% BSA and 0.2% Tween ...
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bioRxiv - Immunology 2021Quote: ... Collagenase D (CAS No. 9001-12-1 from Sigma Aldrich); Masson’s Trichrome (Trichrome Stain Connective Tissue Stain ...
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bioRxiv - Biophysics 2021Quote: ... and 5 mM Trolox (CAS 53188-07-1, Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Dodecanone (CAS #6175-49-1, purity >97%, Aldrich Sigma), (+ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: BPS (Sigma-Aldrich, CAS-No:80-09-1, Purity: 98%) was dissolved in absolute ethanol ...
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bioRxiv - Plant Biology 2022Quote: ... and 1% (v/v) IGEPAL CA-630 (I3021, Sigma-Aldrich)) and incubated for 1 h at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse anti-TUBB3 (1:50, MAB1637, Sigma, CA, USA). The staining and imaging method was the same as the labelling of CGRP and NF ...
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bioRxiv - Plant Biology 2024Quote: ... 0.05% MES Hydrate (Sigma-Aldrich, CAS No. 1266615-59-1), and 0.4% Gelzan (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... and 0.4% Gelzan (Sigma-Aldrich, CAS No. 71010-52-1), and stratified for 72 h at 4℃ in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% Triton X-100 (Sigma, cas# 9002-93-1) rocking gently for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... mecamylamine hydrochloride (M9020-5MG, Sigma-Aldrich, CAS 826-39-1). Drugs were applied to the fly after normal recording (“saline / naïve” ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Igepal ca-360 (Sigma, St. Louis, MI, I-8896), and a protease inhibitor cocktail (Calbiochem ...
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bioRxiv - Plant Biology 2024Quote: ... and 0.7% Gelzan (Sigma-Aldrich, CAS No. 71010-52-1) and stratified for 72 h at 4℃ in the dark ...
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bioRxiv - Plant Biology 2024Quote: ... 0.05% MES Hydrate (Sigma-Aldrich, CAS No. 1266615-59-1), and 0.7% Gelzan (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: STM2457 (Sigma-Aldrich, St. Louis, MO; CAS#2499663-01-1), a selective METTL3 inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... was thawed at 37°C immediately before use and supplemented with 40 μl of 1 mM hemin stock solution and 10 μl of 1 M creatine phosphate (Sigma-Aldrich, Cat: 27920) and 10 μl of 5 mg/ml creatine phosphokinase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... All strains were maintained at −80°C in a 1:1 mixture of 40% glycerol (20% final concentration) and lysogeny broth (LB, Sigma Aldrich, Miller formulation) amended to 3% w/v NaCl (termed LBS 3%) ...
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bioRxiv - Immunology 2024Quote: ... Lysis Buffer (Tris HCl base with 0.1% Tween-20, 0.1% NP-40, 0.01% digitonin, 1 mM DTT, and 1 U/μL Protectors RNase inhibitor, Sigma; full recipe in 10X protocol) was added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Agmatine (CAS no. 2482-0-0) and cisplatin (CAS no. 15663-27-1) were obtained from Sigma Aldrich Corporation (St Louis ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of nuclear extract was mixed with 40 μL anti-Flag M2 affinity agarose slurry (Sigma, #A2220) equilibrated in binding buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... grids were rinsed in PBS and incubated with streptavidin-gold (10 nm, cat# S9059, Sigma-Aldrich, 1:40) 1 h at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.5, 150 mM NaCl, 1mM EDTA, 0.1% Nonidet P-40, 10% glycerol, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 0.1% Nonidet P-40, 1% Plant Proteinase Inhibitor Cocktail [Sigma], and 50 mM 2,2′-Dithiodipyridine [Sigma]) or ...
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bioRxiv - Plant Biology 2020Quote: ... tissue was ground in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 10 mM MgCl2, 150 mM KCl, 1% NP-40 [Sigma I8896-50ML], 0.5 mM DTT [Sigma, 646563] ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins from 7-day-old seedlings were extracted with no-salt lysis buffer (50 mM Tris, pH 8.0, 0.1% Nonidet P-40, and 1% Protease inhibitor cocktail [Sigma]) at a fresh weight:buffer volume ratio of 1 g:2 mL ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Lysis Buffer (150mM NaCl, 50mM Tris-Hcl, pH7.5, 10% Glycerol, 0.5%NP-40, 1mM MgCl2, 1/1000 Benzonase (Sigma), Phosphatase (PPI ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) before eluting in 5 ml of the same buffer plus 40 mM maltose (Sigma Aldrich). The eluate was subjected to ultracentrifugation at 100,000 x g for 1 h at 4 °C to remove any precipitated material and the supernatant was concentrated to the required concentration with 100 kDa cut off vivaspin 500 concentrators (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 1:40 dilution in diluent C of a 1mM stock of PKH26 red fluorescent cell marker (Sigma-Aldrich) for 30 min ...
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bioRxiv - Pathology 2021Quote: ... was homogenized with protein lysis buffer (50mM Tris-HCL pH 8.0, 150mM NaCl, 1% NP-40, 0.5mM PMSF(Sigma) and cOmplete protease inhibitor (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... Dextran (molecular mass 1, 10, 40, 70, 100, 200, 500 and 2000 kDa; Sigma-Aldrich, St. Louis, MO) is added to the culture medium to exert mechanical stress ...
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bioRxiv - Immunology 2020Quote: Whole Cell lysates were prepared with the RIPA buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1mM EDTA with protease inhibitor cocktail [Sigma]), isolated from uninfected ...
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bioRxiv - Immunology 2020Quote: Cells were lysed in RIPA buffer (50 mM Tris.HCl, pH7.4; 150 mM NaCl; 1% NP-40 (Sigma-Aldrich), 0.5% Deoxycholate ...
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bioRxiv - Cancer Biology 2024Quote: ... All cells were lysed in RIPA-buffer (50 mM Tris-HCl pH 8.0, 1 % NP-40 (Sigma, 492018), 0.5 % sodium deoxycholate (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... gels in TAE buffer (40 mM Tris Acetate, 1 mM EDTA pH 8.0) containing ethidium bromide (0.2 mg/mL, Sigma) and imaged on GelDoc DOCTM XR+ Gel Documentation system (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... fluorescence was recorded for ∼100 s before adding 40 μl of 1 M sodium hydrosulfite (Sigma 157953-5G) freshly prepared in 1 M Tris pH 10 ...
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bioRxiv - Cancer Biology 2024Quote: Cell were lysed in RIPA buffer (1X PBS (137mM NaCl; 2.7mM KCl; 4.3mM Na2PO4; 1.47 mM KH2PO4) with 1% Nonidet P-40 Substitute (Sigma #74385), 0.5% Sodium deoxycholate ...
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bioRxiv - Cancer Biology 2022Quote: ... in TAE buffer (40 mM Tris Acetate, 1 mM EDTA pH 8.0) containing ethidium bromide (0.2 μg/mL, Sigma) and imaged on GelDoc DOCTM XR+ Gel documentation system (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml, Sigma-Aldrich, H2519-50UN)/ chondroitinase ABC Lyase (0.1U/ml ...
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bioRxiv - Microbiology 2021Quote: MCF of 1 to 5 m lengths were given an internal hydrophilic coating by incubation with a 5 mg/mL solution of polyvinyl alcohol (PVOH) in water (MW 146,000-186,000, >99% hydrolysed, Sigma-Aldrich, UK) at room temperature for 2h (Pivetal et al. ...
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bioRxiv - Cell Biology 2020Quote: ... with 5 ml fully supplemented media and 100 ng/ml PMA (Phorbol 12-myristate 13-acetate ≥99%, Sigma-Aldrich, Austria) to induce differentiation ...
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bioRxiv - Biophysics 2020Quote: ... followed by 99 μL of TZM-bl cells at 0.1 million cells/ml containing 10 μg/ml DEAE-Dextran (Sigma Aldrich). Final concentrations of the small molecules were 90 ...
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bioRxiv - Microbiology 2022Quote: ... the activity of acetylcholinesterase from the supernatant was calculated using 99% of acetylthiocholine iodide (Sigma-Aldrich, St. Louis, MO, USA) as the substrate in a 2 ml reaction medium consisting of 0.25mM 5 ...
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bioRxiv - Physiology 2021Quote: The assay was performed using the In Vitro Tubulin Polymerization Assay Kit (≥99% Pure Bovine Tubulin; 17-10194 Sigma-Aldrich), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... An o-Phenanthroline solution was prepared mixing 30 μL of hydrochloric acid (37%) with 7 mL MilliQ water and dissolving 0.2 grams 1,10-Phenanthroline monohydrate (Sigma-Aldrich, 99%) in it ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...