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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for 8 Allyloxycarbonyl amino 3 6 dioxaoctanoic acid dicyclohexylamine Aloc Ado*DCHA Aloc AEEA*DCHA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: Defined minimal media contained 6.7 g/L of yeast nitrogen base without amino acids (cat# Y0626, Sigma-Aldrich, MO, USA), 20 g/L of glucose ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Neuroscience 2019Quote: Tau variants (full length protein and a fragment encoding amino acids 256-368) were cloned into the pET19b vector (Novagen) in between the NcoI and BamHI restriction sites ...
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bioRxiv - Genomics 2020Quote: ... embryoid bodies were transferred to ultra-low-attachment 6-well TC plates in neural induction media (DMEM/F12, 1% Glutamax, 1% non-essential amino acids and 10 µg/ml heparin (Sigma). After another 4-5 days ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then grown for ≈2 generations (OD600 ≈0.2) before amino acid starvation was induced by the addition of either 500 μg/ml valine (Sigma Aldrich) or 400 μg/ml serine hydroxamate (Sigma Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... Then 1 × 107 cells were washed with sterile water and transferred to nitrogen starvation conditions [6.7 g/L YNB without amino acids and without ammonium sulfate (Sigma-Aldrich) and 20 g/l glucose] and grown at 30 °C and 200 rpm ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Microbiology 2021Quote: ... cell cultures were diluted in 5 ml of M9 with 0.4% glucose and 0.08% RPMI 1640 amino acids (Sigma-Aldrich R7131), and incubated in 37°C (strain EL2504) ...
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bioRxiv - Immunology 2021Quote: ... HLA typed HLA-A*02:01+ PBMCs from COVID-19 recovered individuals were incubated with SARS-CoV-2 peptide pools (2µM / peptide) and cultured for 10-14 days in RPMI-1640 supplemented with 1x Non-essential amino acids (NEAA; Sigma), 5 mM HEPES (Sigma) ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...
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bioRxiv - Cell Biology 2022Quote: ... 1% penicillin/streptomycin and 1% MEM non-essential amino acid solution (all three from Sigma-Aldrich, St. Louis, MO, USA) in 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Microbiology 2022Quote: ... Yeast were grown on 2% (w/v) agar plates containing yeast nitrogen base (YNB) without amino acids (6.8 g/l, Sigma-Aldrich) supplemented with yeast synthetic drop out (YSDO ...
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bioRxiv - Molecular Biology 2022Quote: ... yejASm lacking the fragment encoding the first 30 amino acids (signal peptide) was PCR-amplified from Sm1021 genomic DNA and cloned into a pET29b(+) vector (Novagen) generating a C-terminal 6His-tag ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown in M9-glucose minimal medium (0.4% D-glucose, 1× MEM amino acids and 1× MEM vitamin) prepared with ingredients from Sigma (57). Antibiotics were used at the following concentrations ...
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bioRxiv - Molecular Biology 2022Quote: ... where individual amino acids concentrations are as defined in Yeast Synthetic Drop-out Media Supplements (Sigma-Aldrich, Saint Louis, Missouri), and 2% Glucose ...
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bioRxiv - Plant Biology 2022Quote: ... Dried samples were then resuspended in 150 µL methanol containing internal standards (15 µM U-13C, 15N amino acid mix, 5 µg/mL 13C2, 15N3 cytosine (Sigma), 10 µg/mL U-13C mannitol and trehalose (Omicron) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast strains were grown in synthetic dropout (SD) media containing Yeast Nitrogen Base Without Amino Acids mix (Sigma-Aldrich Y0626) supplemented with 10 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: ... Polar metabolites were resuspended in 170 µL of 100% methanol containing 13C-15N labeled amino acids (30 µM, 767964, Sigma). UHPLC normal phase chromatography was performed using an Agilent 1290 LC stack ...
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bioRxiv - Plant Biology 2023Quote: ... then resuspended in 100% MeOH containing an internal standard mix of isotopically labeled compounds (∼15 µM average of 5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 10 µg/mL 13C-trehalose ...
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bioRxiv - Neuroscience 2023Quote: ... gut samples were treated with D-amino acid oxidase from porcine kidney (PkDAAO) (Catalog# A5222, Sigma-Aldrich, St. louis, MO). The sample was mixed with PkDAAO (15 U/mL) ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich), 1 g/L monosodium glutamate (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Viral inoculum was replaced with 0.22 μm filter-sterilized pH 6.8 growth medium (1X non-essential amino acids [Gibco, MA]/20 mM HEPES, pH 6.8 [Gibco, MA]/5 mM sodium bicarbonate [Sigma-Aldrich, MA]/1X penicillin-streptomycin [Gibco ...
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bioRxiv - Microbiology 2023Quote: The cDNA for AFF4 (also known as MCEF) amino acids 1-715 was cloned into pet-6HIS- 11d plasmid (Novagen) and expressed in E ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were grown at 30 °C in minimal synthetic media or agar plates containing 6.8 g/L yeast nitrogen base without amino acids (Sigma-Aldrich), 2 g/L dextrose (Formedium) ...
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bioRxiv - Bioengineering 2023Quote: ... yeast cultures were grown in synthetic complete media containing 0.67% (w/v) Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich), 1.92 g/L Yeast Synthetic Media Dropout Mix (uracil ...
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bioRxiv - Genetics 2024Quote: Serial 10-fold dilutions of logarithmic yeast cells were spotted on fresh synthetic dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of methyl methanesulfonate (MMS) (Sigma) and incubated at 30°C for 3 days ...
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bioRxiv - Biochemistry 2023Quote: ... DNA encoding the amino acids 264-1309 of dmIR was subcloned into the vector pBAC™4x-1 DNA (Novagen) downstream of the p10 promoter with the addition of a starting Methionine and C-terminal StrepII-tag ...
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bioRxiv - Microbiology 2024Quote: ... to introduce a KpnI site without changing the amino acid sequence by PCR from pTagRFP-N1 65 and cloning it into pFlag-CMV2 (Sigma). pFlag-TagRFP-KanS ...
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1 µg/mL) and the cells were washed three times with PBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... except 6-AM which was dissolved in 1% lactic acid (Sigma Aldrich, St. Louis, MO). Drugs were administered SC or IV at an injection volume of 1-2 ml/kg and all drug doses were expressed as the salt forms listed except for 6-AM which was expressed as the base form ...
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bioRxiv - Biophysics 2021Quote: ... and 1 mM (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; 238813-5G, Sigma-Aldrich). To obtain images for training the DeepSTORM model ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 % 6-acrylamidohexanoic acid (Aladdin) in ddH2O with 0.004 g ml−1 TEMED (Sigma) and 0.001 g ml−1 ammonium persulfate (Sigma) ...
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bioRxiv - Biophysics 2019Quote: ... and aged 1.5 mM 6-hydroxy-2,5,7,8-tetramethyl-chromane-2-carboxylic acid (Trolox, Sigma-Aldrich) was added to the final single-molecule imaging solutions to reduce fluorophore photobleaching and blinking [46].
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bioRxiv - Biophysics 2020Quote: ... 1 % penicillin-streptomycin and 1.5 mg/ml 6-aminocaproic acid (ACA, Sigma, St. Louis, USA) was added ...
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bioRxiv - Synthetic Biology 2023Quote: ... their pH was tuned with hydrochloric acid (HCl, 1 M or 6 M, Sigma-Aldrich) or potassium hydroxide (KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... the differentiation media was supplemented with 6 μM all-trans retinoic acid (RA, Sigma-Aldrich) until day 8 ...
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bioRxiv - Cell Biology 2021Quote: ... 2M urea or no urea using a D-Tube Dialyzer MWCO 6-8 kDa (Millipore Sigma). NFL assembly (0.24 μg/μl total ...
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bioRxiv - Immunology 2024Quote: ... 8 µM 6-biopterin,1 mg/L biotin and 1x RPMI 1640 vitamins solutions (Sigma, USA). Parasites in culture were passaged to fresh medium at a one 20th-50th dilution once a week.
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bioRxiv - Immunology 2021Quote: ... or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU) (courtesy of Jeffrey Aubé, UNC) and 50 μM/ml Methylglyoxal (Sigma). Brefeldin A Solution (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Genomics 2020Quote: ... before addition of 500 μM indole-3-acetic acid solution (“auxin”, Sigma-Aldrich) for different times to induce RPB1 degradation ...
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bioRxiv - Genomics 2020Quote: ... To the XRN1-AID strain 0.2mM of auxin (3-Indoleacetic acid; Sigma-Aldrich) was added to the media 30 and 60 minutes before harvesting ...
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bioRxiv - Cell Biology 2021Quote: ... were treated with 500μM IAA (indole-3-acetic acid dissolved in DMSO; Sigma) to induce degradation of the desired AID-tagged target protein ...
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bioRxiv - Biophysics 2020Quote: ... 4.5% acetic acid and 2.5% 3-(triethoxysilyl)-propylamine (EMD Millipore Corp., Billerica, MA), rinsed with methanol and water and finally dried under a stream of nitrogen (63) ...
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bioRxiv - Physiology 2022Quote: Auxin plates were prepared by adding auxin indole-3-acetic acid (Sigma-Aldrich) from a 400 mM stock solution in ethanol into NGM at the final concentration of 1 mM (Zhang et al ...