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Citations for Millipore Sigma :
51 - 100 of 103 citations for pG2 er since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and the ER was labeled with antibodies against CALNEXIN (Mo, Millipore, MABF2067, 1:200) or RRBP1 (Mo ...
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bioRxiv - Synthetic Biology 2021Quote: ... ER was grown in ABB media with the addition of 3.3 mM sodium acetate (Sigma) and RI was grown in Brain Heart Infusion Broth (BHI ...
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bioRxiv - Molecular Biology 2021Quote: ... MCF10a-Snail-ER cells were treated with 3µg/mL of TSA (Trichostatin A - Sigma T8552) or 10nM of Panobinostat (gift from Moreaux Lab ...
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bioRxiv - Molecular Biology 2022Quote: ... ER stress was induced by treating cells with either 400 nM Thapsigargin (Tg) (Sigma-Aldrich) or 100 µM Cyclopiazonic acid (CPA ...
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bioRxiv - Microbiology 2023Quote: ... transepithelial electric resistance (TEER) measurements were performed using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01 ...
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bioRxiv - Neuroscience 2024Quote: ... ER stress was induced by treatment of cells with 10 µg/ml tunicamycin (Sigma, T7765) or 1 µM thapsigargin (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... TEER was measured using the Millicell-ERS (Electrical Resistance System) epithelial volt-ohmmeter (Millipore Sigma) and STX2 chopstick electrode (WPI ...
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bioRxiv - Systems Biology 2022Quote: ... Transendothelial electrical resistance (TEER) measurements were performed using a Millicell ERS-2 Voltohmmeter (Millipore, Watford, UK) and were expressed as Ω.cm2 ...
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bioRxiv - Microbiology 2020Quote: ... the TER of the monolayers on the filter was measured using a Millicell-ERS device (Millipore), and monolayers with measured TER values of 450–500 Ω/cm2 were used in experiments ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transepithelial electrical resistance (TEER) was measured using a Millicell-ERS resistance system (Millipore, Bedford, MA, USA). Cell monolayers used for transport studies had TEER values of 800 to 1000 Ω·cm2.
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bioRxiv - Cell Biology 2020Quote: To induce ER stress cells were incubated in medium containing 1 μM Tunicamycin (Sigma Aldrich, T7765) or 1 μM Thapsigargin (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: Contact between ER and lysosome was examined using Duolink In Situ Detection Reagents Red kit (Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... CGRP-ER-TDT mice were induced by injecting 100mg/kg tamoxifen (Sigma-Aldrich, St. Louis, MO) intraperitoneally ...
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bioRxiv - Biophysics 2021Quote: ... ER-Hoxb8 cells were cultured in uncoated six-well culture plates in RPMI 1640 medium (Merck Millipore) supplemented with 10% FBS (Pan Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... The transendothelial electrical resistance (TEER) of the HUVEC monolayers was measured with Millicell-ERS volt-ohmmeter (Millipore) and reported as percentage difference between TEER values before and after infection/treatment.
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bioRxiv - Neuroscience 2024Quote: ... Trans-endothelial electrical resistance (TEER) was measured using a Milli-cell-ERS electrode from Millipore (Billerica, USA). Calibration of the electrode in reference media was performed before measurements and values from transwells without cells served as blank controls.
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bioRxiv - Cell Biology 2024Quote: ... TEER measurements were performed under sterile conditions using a Millicell ERS-2 Voltohmmeter (Millipore, Burlington, MA, USA). The culture medium was replaced before each TEER measurement ...
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bioRxiv - Cell Biology 2024Quote: ... Culture media was replenished 30 minutes prior to measuring TEER with a Millicell ERS-2 voltohmeter (Millipore), following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells stably expressing the ER-phagy probe were cultured with 0.5 μg/mL doxycycline (D3447; Sigma-Aldrich) for 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Setd2flox/flox MEFs expressing ER-Cre were treated with 3μM (Z)-4-hydroxytamoxifen (4-OHT, Millipore Sigma, H7904) or vehicle (Ethanol ...
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bioRxiv - Genomics 2023Quote: ... was aliquoted into each of 3 tubes with antibody (1.25 µg anti-HA, Cell Signaling #3724S, 2.5µg anti-ER, Millipore #06-935 ...
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bioRxiv - Microbiology 2023Quote: Transepithelial electrical resistance (TEER) of the filter-grown monolayers was measured using a Millicell-ERS device (Merck Millipore) as an index for integrity of the tight junctions (TJ).
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bioRxiv - Cell Biology 2022Quote: ... Unhooking of RUSH-TfR from the ER was allowed by the addition of D-biotin (Sigma-Aldrich, B4501-500MG) at 100 μM final concentration in DMEM for 30 min prior fixation ...
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bioRxiv - Plant Biology 2020Quote: ER stress treatments of Arabidopsis seedlings were performed by growing seeds directly on MS plates supplemented with TM (Sigma) or DTT (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... Transepithelial electrical resistance (TEER) was quantified cultures using the Millicell Electrical Resistance System ERS-2 Volt-Ohm Meter (Millipore).
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bioRxiv - Microbiology 2021Quote: ... Polarization of Calu-3 cells was evaluated by measuring the transepithelial resistance (TER) using a Millicell ERS meter (Millipore). Cells were considered polarized when TER values reached 500-600 Ωcm-2.
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bioRxiv - Microbiology 2024Quote: ... Electrical resistance (Ωt) was quantified using Ag/AgCl electrodes connected to a Volt-Ohm meter (Millicell ERS-2, Millipore), and this value was then transformed into a TEER measurement using the following equation ...
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bioRxiv - Cell Biology 2020Quote: MDCK cells were grown on collagen-coated transwell filters (see Cell Culture section) and resistance measurements were taken four days after plating with a Millicell ERS-2 Voltohmmeter (Millipore). Three measurements per well ...
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bioRxiv - Neuroscience 2020Quote: The barrier integrity of the in vitro BBB models was assessed through measurements of TEER using Millicell ERS-2 epithelial volt-ohm Meter and STX01 Chopstick Electrodes (Merck Millipore). The TEER value for each hanging culture insert was obtained from an average of three individual measurements subtracted the TEER value of a double-coated cell-free hanging culture insert and multiplied by the area of the hanging culture insert (1.12cm2) ...
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bioRxiv - Microbiology 2020Quote: ... used for barrier dysfunction experiments were conducted on a 37 °C heating block using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01) ...
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bioRxiv - Molecular Biology 2021Quote: TEER measurements [22] were performed on 3rd day of incubation with growth factors and (or) different inhibitors by using Millicell ERS-2 Electrical Resistance System (Merck-Millipore). Each insert was measured in three different locations ...
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bioRxiv - Biophysics 2022Quote: ... To clear the golgi and ER content GFP-GPI and VsV-G-EGFP expressing cells were exposed to 50 μg/ml of cycloheximide (Sigma) for a total of 3-4 hours before the experiment.
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bioRxiv - Cell Biology 2022Quote: ... Fraction 5/6 (found at the interface between 22.5% and 9.5% Nycodenz and enriched in ER and autophagosome membranes (Strømhaug et al., 1998)) was diluted 1.25X and loaded on a Percoll (Sigma-Aldrich; P4937)/Nycodenz gradient (110 μL of 22.5% Nycodenz on bottom ...
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bioRxiv - Microbiology 2023Quote: ... The basolateral media was then replaced with 500 μl PBS and another 200 μl PBS applied to the apical surface before measuring the transepithelial electrical resistance (TEER) using the Millicell Electrical Resistance System (ERS)-2 (Sigma). Following TEER measurements ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Transepithelial Electrical Resistance (TER) detected by using a Millicell-electrical resistance system (ERS)-2 Volt-ohm meter (Cat# MERS00002, Millipore) was used to evaluate the integrity of the Sertoli cell TJ-permeability barrier ...
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bioRxiv - Cell Biology 2024Quote: ... Expanded colonies were tested by inducing ER stress with 200 nM thapsigargin for 6 h (Sigma-Aldrich, Burlington, MA, USA) using confocal microscopy (Zeiss LSM800 controlled by the ZEN 2.3 software ...
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bioRxiv - Pathology 2024Quote: ... was transduced into EA.hy926 cells using lentiviral transfection.28 ER stress was induced by 1 µg/mL tunicamycin (Sigma-Aldrich) and the cells were also incubated with KUS121 or 5 % glucose solution as control ...
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bioRxiv - Molecular Biology 2024Quote: ... SH-EP-MYCN-ER cells were infected with lentiviral supernatants in the presence of 4 µg/ml polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... The electrical resistance across the cell monolayer was monitored daily from the second day onwards for 7 days using the Millicell-ERS epithelial volt-ohm meter (Millipore). Transepithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2021Quote: TEER across cellular monolayers was measured with chopstick electrodes in 24-well Transwell inserts using the Millicell® ERS-2 Voltohmmeter (Merck Millipore). The absolute TEER of eGFP-hCldn5-MDCK II cells before treatment was recorded as a baseline reading ...
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bioRxiv - Genomics 2021Quote: ... 6 ml of ER-HoxB8 cell culture media (RPMI 1640 supplemented with 10% FBS, 1% penicillin/streptomycin, 0.5 μM β-estradiol (Sigma-Aldrich), and 20 ng/ml GM-CSF (PeproTech) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were considered to be confluent and polarised on the polycarbonate membrane of Transwell inserts when a stable measurement of the transepithelial electrical resistance (TEER) using the Millicell ERS-2 Volt-Ohm meter (EMD Millipore) reached 500∼680 Ω.cm2 ...
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bioRxiv - Microbiology 2024Quote: Cytotoxicity was studied according to the manufacturer’s instruction either using cytotoxicity LDH assay kit-WST (Dojindo) or by measuring transepithelial electrical resistance (TEER) (Millicell® ERS-2, Millipore) as previously described (25) ...
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bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The reporter proteins were released from the ER-localized streptavidin ‘hook’ with the addition of 40 μM biotin (Sigma-Aldrich, B4639-100MG) to the coverslip ...
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bioRxiv - Physiology 2022Quote: ... Potential difference and transepithelial resistance were measured in the absence of any drug (except otherwise noted) using a Millicell-ERS Volt-Ohm meter (Millipore, Billerica, MA).
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bioRxiv - Bioengineering 2024Quote: ... was assessed every day for 7 to 10 days using a Millicell ERS-2 voltammeter EVOM2 and an STX02 chopstick electrode (Merck Millipore, Bayswater Australia). The TEER value was calculated by subtracting the resistance of a blank insert from an insert containing a monolayer and then multiplying it by the surface area of the insert.
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bioRxiv - Neuroscience 2020Quote: ... Nuclear localization of Cre recombinase in Ntrk2Cre-ER/+ mice was induced by tamoxifen (20 mg/ml in corn oil, Sigma-Aldrich T-5648) administration for 6 days [3 mg per animal per day ...
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bioRxiv - Cell Biology 2022Quote: ... electric resistance between the apical and basal compartments was measured each day using a volt-ohm meter Millicell ERS-2 (EMD Millipore, MA, USA). The electric resistance of a blank filter was subtracted from each measurement ...
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bioRxiv - Microbiology 2023Quote: Barrier integrity was assessed using Trans-Epithelial Electrical Resistance (TEER) of polarized monolayers measured using the Millicell-Electrical Resistance System (ERS, Millipore, Billerica, MA, USA) and those with a minimum TEER of 200 Ο/cm2 (range ...