Labshake search
Citations for Millipore Sigma :
51 - 100 of 4613 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... The chromatin pellet was digested in 100 μl of nuclease-free water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 minutes at 37°C in a thermomixer at 1,400 rpm ...
-
bioRxiv - Neuroscience 2021Quote: ... Injection solutions were prepared with a final concentration of 300ng/µl nCas9n mRNA and 10ng/µl sgrNA in nuclease free water and 0.05% (w/v) phenol red (Sigma Aldrich). Wildtype embryos were injected at the single or two cell stage with 1.5nL injection solution ...
-
bioRxiv - Developmental Biology 2021Quote: ... They were then transferred to another cavity containing 2.5 % Liberase (5 g Liberase reconstituted in 2ml nuclease free water; Sigma 5401119001) and teased apart with tungsten needles until most of the clumps were separated and left (without agitation ...
-
bioRxiv - Immunology 2023Quote: ... Both insert and backbone were column purified according to the manufacturer’s instructions (D4033) and drop dialyzed for 2 h using nuclease-free water (Millipore VSWP02500). The RBD library insert and linearized pYD1 backbone were co-transformed into yeast (S ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 μL cDNA template at a concentration of 10 ng μL-1 and 3.5 μL of nuclease-free water (Sigma-Aldrich). No template controls were included for all reactions ...
-
bioRxiv - Biophysics 2024Quote: ... was then used to prepare a 10 μM solution in nuclease-free water with 1 mM phosphate buffer (Sigma P3619) at pH 7.4 and 25 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... EDTA Free (Pierce™) and Benzonase Nuclease (NOVAGEN) as recommended by the manufacturer ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.15 mg/mL nuclease-free BSA (Merck-Millipore), 2 mM DTT ...
-
bioRxiv - Cell Biology 2020Quote: ... the channel was washed three times with 10 μl nuclease-free water and the channel walls were passivated with 10 mg/ml Western Blocking Reagent (Sigma Aldrich) in PBS for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: ... 250, 300, or 800 ng/μL by using nuclease-free water (Cytiva, SH30538.01) containing 0.05% phenol red (SIGMA-ALDRICH, P5530-5G) as an injection marker.
-
bioRxiv - Cell Biology 2023Quote: ... The volume was brought to 250μL with nuclease-free water and RNA was extracted with 24:1 Chloroform-Isoamyl alcohol (Sigma-Aldrich, #227056) using a MaXtract tube ...
-
bioRxiv - Molecular Biology 2019Quote: ... and eluted in nuclease-free EmbryoMax microinjection buffer (Millipore).
-
bioRxiv - Microbiology 2019Quote: ... no vigorous mixing steps (performed by inversion) and elution into 50μl of double processed nuclease free water (Sigma-Aldrich, St. Louis, USA). DNA was quantified using a Qubit and the HS (High sensitivity ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue from three rats per sex was combined and transferred to 5 ml of ice cold lysis buffer (10 mM tris-HCl, 10 mM NaCl, 3 mM MgCl2 and 0.1% Igepal in nuclease-free water (Sigma-Aldrich, 18896-50ML) for 15 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Certified RNase-free water (Sigma W3513) was used for elution and storage of RNA ...
-
bioRxiv - Genomics 2019Quote: ... with minor alterations including no vigorous mixing steps (performed by inversion) and elution into 100μl double processed nuclease free water (Sigma-Aldrich, St. Louis, USA). gDNA for each extract was quantified using a Qubit and the HS (high sensitivity ...
-
bioRxiv - Microbiology 2021Quote: ... A calibrated loop (1 μL) was used to suspend colony material in 600 μL nuclease free water (Sigma-Aldrich, St Louis, MO, USA) and mixed with 0.1 mm silica beads (BioSpec Products Inc. ...
-
bioRxiv - Physiology 2021Quote: ... and tracrRNA were resuspended to 100 μM in nuclease-free and sterile T10E0.1 buffer (10 mM Tris-HCl, 0.1 mM EDTA, embryo-tested water, Sigma, W1503, St. Louis, Missouri, USA) prepared as described (51) ...
-
bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
-
bioRxiv - Genomics 2023Quote: ... staining base was then made in molbio grade water to a final concentration of 1% w/v of Nuclease Free BSA (Sigma-Aldrich, 126609-100GM), 1x HBSS (gibco ...
-
bioRxiv - Molecular Biology 2023Quote: ... then treated with nuclease-free Pronase (Millipore; 46 mg total), and placed in 540 ml YPD medium at 18°C ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 3.25 µL RNase-free water (Sigma-Aldrich), and 1 µL of dNTP mix (10 mM each ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 2 µL RNase-free water (Sigma-Aldrich), and 2.5 µL of dNTP mix (10 mM each ...
-
β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... free nucleic acids were digested with Benzonase® Nuclease (70746, Millipore) and leftover cellular components were discarded after centrifugation ...
-
bioRxiv - Systems Biology 2022Quote: ... + 0.04% Nuclease-Free Bovine Serum Albumin (BSA, EMD Millipore, Cat # 12661525mL). Cells were resuspended to a target concentration of 1,000 cell/µL ...
-
bioRxiv - Biophysics 2020Quote: ... were fixed with 4% (v/v) methanol-free paraformaldehyde in nuclease-free phosphate saline buffer (PBS, Sigma) for 10 min at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... in RNase-free water (Sigma-Aldrich, ca. 95284). Next ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 μL of RNAse-free water (Sigma-Aldrich) were added to dissolve the pellet ...
-
bioRxiv - Cell Biology 2022Quote: ... before incubation with blocking buffer (3% nuclease-free bovine serum albumin (Sigma) in PBS ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 ml of chloroform: isoamyl alcohol (24:1, nuclease-free, Sigma®) was added to the tube and inverted several times to form an emulsion to remove residual phenol ...
-
bioRxiv - Microbiology 2023Quote: ... All mIgGs administered to mice were purified using buffers made with endotoxin-free water (HyPure Cell Culture Grade Water, Cytiva SH30529.03) and were dialyzed into endotoxin-free Dulbecco’s PBS (EMD Millipore, TMS-012-A). Following dialysis ...
-
bioRxiv - Synthetic Biology 2021Quote: ... followed by drop dialysis for 1 hour in nuclease-free H2O (Millipore VSWP02500). The libraries were cloned and expressed in yeast by in vivo homologous recombination ...
-
bioRxiv - Biochemistry 2023Quote: ... Free genomic and plasmid DNA was digested with a Benzonase Nuclease (Sigma-Aldrich). Cell debris was removed by centrifugation and ...
-
bioRxiv - Microbiology 2021Quote: ... and DNA/RNA-free water (2 µl; Sigma-Aldrich) at 95°C for 2 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protease free water (Molecular Biology Reagent, Sigma-Fine Chemicals).
-
bioRxiv - Neuroscience 2023Quote: ... in water (free of RNAase and DNAase; Sigma-Aldrich). The second wash was with 1:1 mix of MABT (1.2% maleic acid ...
-
Driving Osteocytogenesis from Mesenchymal Stem Cells in Osteon-like Biomimetic Nanofibrous ScaffoldsbioRxiv - Bioengineering 2022Quote: ... an equivalent volume of DNase and RNase-free water (Sigma) was used in place of RT Enzyme Mix ...
-
bioRxiv - Neuroscience 2022Quote: ... DNA was resuspended and solubilized in DNAse-free water (Sigma-Aldrich) and sequenced on Illumina Hi-Seq machines at the Yale Center for Genome Analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3.25 µl sterile DNase/RNase free water (Sigma, ST. Louis, USA) and 400nM of all forward and reverse primers ...
-
bioRxiv - Immunology 2021Quote: ... RNA was eluted in 35 μl of RNAse-free water (Sigma).
-
bioRxiv - Developmental Biology 2019Quote: ... All solutions were diluted in RNAse-and DNAse-free water (Sigma). Injection volume is 1nl per embryo for all experiments.
-
bioRxiv - Neuroscience 2023Quote: ... completed with molecular water (free of RNAase and DNAase; Sigma-Aldrich).
-
bioRxiv - Neuroscience 2023Quote: ... completed with molecular water (free of RNAase and DNAase; Sigma-Aldrich).
-
bioRxiv - Microbiology 2022Quote: ... The pellet was then washed twice with ice-cold 70% nuclease-free ethanol (Sigma-Aldrich, 51976). Finally ...
-
bioRxiv - Biochemistry 2019Quote: All buffers were prepared using Ca2+-free water for molecular biology (Millipore). Plasmids carrying the WT or mutant genes were transformed into the S ...
-
bioRxiv - Microbiology 2019Quote: ... LTA (200 ng/ml) was dissolved in endotoxin free water (Sigma Aldrich) with melimine or Mel4 at their MICs or MBCs as final concentrations and incubated for 1-2 h at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... mixed with RNase-free water and proteinase K (100 μg/mL, Sigma) and incubated for 10 minutes at 55°C ...
-
bioRxiv - Microbiology 2021Quote: ... the free RNA not associated with virions was removed from the samples using the Benzonase nuclease (Sigma), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... and pH 7.4 using endotoxin-free ultra-pure water (TMS-011-A, Sigma) and 12–14 kDa MW cut-off membranes (ZelluTrans/Roth T3) ...
-
bioRxiv - Neuroscience 2023Quote: ... the RNA was dissolved in 20-50 μL of RNase-free water (Sigma) and incubated for 10 min at 60°C ...