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Citations for Millipore Sigma :
51 - 100 of 8956 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Protein was detected by enhanced chemiluminescence (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by enhanced chemiluminescence (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Protein was detected by enhanced chemiluminescence (Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescent yellow‐green latex beads (SIGMA-Aldrich) at a concentration of 8×106 beads/well (∼100 beads/cell ...
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bioRxiv - Neuroscience 2024Quote: ... fluorescent yellow-green latex beads (Sigma-Aldrich) were added to iMicroglia cultures treated with or without EVs ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Cancer Biology 2020Quote: The puromycin selection gene was replaced with green fluorescent protein (eGFP) cDNA in Lentiviral pLKO.1 plasmid (Sigma). shRNA oligos (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Protein bands were visualized using enhanced chemiluminescence reagents (Millipore, WBKLS0500).
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... peroxidase-anti-peroxidase for detecting TAP-tagged proteins (Sigma); anti-FLAG mouse monoclonal antibody (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... HTP-tagged proteins were detected using rabbit PAP (Sigma) diluted 10,000 fold ...
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme) ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with an enhanced chemi-luminescence system (Millipore Luminata) and visualized using an Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins expression was detected using an enhanced chemiluminescence kit (EMD Millipore).
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by using enhanced chemiluminescence (ECL) systems from Millipore or GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo, Merck Millipore). Membranes were re-probed with β-Actin antibody to ensure equal loading.
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Bioengineering 2023Quote: We verified the patterning and the pattern transfer to PA hydrogel using green fluorescent laminin (as described in Protein patterning glass coverslips) and a pan-cadherin primary antibody (Sigma, C3678). We diluted the pan-cadherin antibody 1:200 in PBS and incubated on the devices for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Plant Biology 2023Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST tag (Step 2 ...
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bioRxiv - Biochemistry 2019Quote: For fluorescent labeling of Ag85B the green fluorescent dye CFTM 488A succinimidyl ester (Sigma-Aldrich, St. Louis, USA) was used ...
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bioRxiv - Neuroscience 2023Quote: ... treated microglia were incubated with fluorescent latex beads of 1 μm diameter (green, fluorescent 496/519; Sigma-Aldrich) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: Exosomes were labelled using the green lipophilic fluorescent dye PKH67 (Sigma) for five minutes and the reaction was stopped by the addition of exosome-depleted FBS ...
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bioRxiv - Neuroscience 2021Quote: Aqueous green fluorescent latex beads of 1µm diameter (Sigma-Aldrich, L1030) were pre-opsonised in FCS in a ratio of 1:5 for 1 hour at 37°C ...
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bioRxiv - Physiology 2023Quote: ... and PKH67 green fluorescent cell linker (1:250, MIDI67, Sigma, USA) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoreactive proteins were detected by an enhanced chemiluminescence detection system (EMD-Millipore).
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by ECL (Enhanced chemiluminescence; Merck Millipore, Cat. No. WBKLS0500). The signal was detected using Vilber FUSION-SL system ...