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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-Parvalbumin (Sigma-Aldrich, P3088, 1:2,500) were used as primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-parvalbumin antibody (Sigma, P3088, 1:1000). After 3 rinses in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections labeled with mouse anti-parvalbumin (PV; Sigma #P3088) were incubated at 1:1000 overnight at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-parvalbumin (Cat. #P3088, Sigma-Aldrich; 1 :1000), rabbit anti-somatostatin (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with anti-mouse NeuN or anti-mouse parvalbumin (Millipore, MAB377 ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti c-myc tag (Clone 9E10 1:100) from Sigma-Aldrich; rabbit anti DsRed (Polyclonal 1 ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2024Quote: ... His-tag batch purification was performed using His-select nickel-affinity gel (Sigma-Aldrich). Purified proteins were detected on a 10% acrylamide gel (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were purified by His-affinity chromatography (cOmplete His-Tag Purification resin, Sigma #5893682001) from the supernatant ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Biophysics 2024Quote: ... and H3.1 were subcloned into a pET15b-derived plasmid containing an N-terminal 6×His tag (MGSSHHHHHHSSG) in which the thrombin cleavage site of pET15b (Merck Millipore, 69661) was replaced by the TEV protease recognition sequence ...
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bioRxiv - Neuroscience 2022Quote: ... parvalbumin (Millipore, MAB1572), GAD65/67 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... either 1:200 goat anti-choline-acetyl-transferase from Millipore or 1:500 mouse anti-parvalbumin monoclonal IgG1 from Sigma-Aldrich in PGT at room temperature for 48 h or 2 h respectively ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-parvalbumin (anti-PV, 1:500, Sigma-Aldrich, P3088, RRID:AB_477329), rat anti-somatostatin (SST ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunofluorescence with anti-parvalbumin (Merck Millipore MAB1572, mouse IgG1. 1:100) and anti-Sox2 (Abcam ab92494 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-HA tag (Clone 6E2, Millipore).
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bioRxiv - Cell Biology 2021Quote: ... FLAG-tag (clone M2, SIGMA-ALDRICH), FLAG-tag (Thermo ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% glycerol) supplemented with His-tag protease inhibitors (Sigma-Aldrich). Samples were sonicated twice using a MSE Soniprep 150 apparatus (Sanyo ...
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bioRxiv - Neuroscience 2020Quote: ... a mouse anti-parvalbumin polyclonal antibody (1:1000, catalog: P3088, Sigma Aldrich) and a rabbit anti-vasoactive intestinal peptide (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: parvalbumin (mouse, 1:500, Millipore MAB1572), COX-IV (rabbit ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-parvalbumin (1:1000, cat #P3088, Sigma-Aldrich, St. Louis, MO), rabbit anti-S100a1 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... we mainly used anti-parvalbumin (PV; monoclonal mouse IgG1, P3088, Sigma-Aldrich), diluted 1:16000 ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... or a VSVG epitope tag on the N-terminal tag of H2A (Sigma, V4888) and a fluorescently labelled secondary antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... Nickel resin for purification of His-tagged proteins was purchased from Sigma-Aldrich (cOmpleteTM His-Tag resin). All lipids ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-HA-tag (clone 3F10, Sigma, #11867432001), anti-giantin (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... and mouse anti-His (Novagen).
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bioRxiv - Neuroscience 2023Quote: ... PV: Monoclonal Anti-Parvalbumin antibody produced in mouse (1:1000, Sigma, Cat# P3088), GluR2/3 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated in mouse anti-parvalbumin antibody (1:400; EMD Millipore, MAB1572) in blocking solution (PBS supplemented with 0.1 % Triton-X 100 and 1% normal goat serum ...