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Citations for Millipore Sigma :
51 - 100 of 3486 citations for GMPR Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The rHA proteins have a molecular weight of ∼270 kDa and were expressed in HEK-293 cells and purified using IMAC Sepharose® High Performance resin (Sigma Aldrich, St. Louis, MO). Viruses were from the Influenza Reagents Resource (IRR ...
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bioRxiv - Microbiology 2019Quote: Human embryonic kidney HEK-293T were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma F8067) and gentamycin (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... The Madin Darby canine kidney (MDCK) and human embryonic kidney (HEK) 293T cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Sigma), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were cultured in DMEM (high glucose, Sigma-Aldrich, SH30022.01) with 10% FCS (Hyclone ...
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bioRxiv - Microbiology 2023Quote: ... University of Lausanne) and Human Embryonic Kidney (HEK) 293T cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, cat. no. D6429) supplemented with 10% heat-inactivated foetal bovine serum (hiFBS ...
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bioRxiv - Cell Biology 2023Quote: ... and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Microbiology 2020Quote: ... or 293-Free (EMD Millipore), according to the manufacturer’s instructions (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... TIFs (Telomerase immortalised fibroblasts29) and human embryonic kidney (HEK)293Ts cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (D5796, Sigma). All cell culture medium was supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Human HEK-293T cells were grown in Dulbecco’s Modified Eagle’s high glucose medium with HEPES (Sigma-Aldrich®, D6171) supplemented with 10% fetal bovine serum ...
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bioRxiv - Genetics 2021Quote: Human embryonic kidney (Hek) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Biochemistry 2020Quote: ... adherent human embryonic kidney (HEK) 293T cells were transfected with the expression plasmids with branched polyehtyleneimine (PEI, Sigma-Aldrich). In detail ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Biophysics 2024Quote: The tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK) 293T cells and human non-small-cell lung cancer (NSCLC) A549 cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Microbiology 2020Quote: ... or 293CD4 cells (293 cells constitutively expressing human CD4) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Physiology 2021Quote: Ion channel genes were expressed in human embryonic kidney (HEK) tsA cells obtained from two sources (Prof. R. Horn, Jefferson University, Philadelphia, PA and Sigma-Aldrich #96121229) as described previously (20) ...
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bioRxiv - Physiology 2023Quote: Frozen aliquots of tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO) and stored in liquid nitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoproteinbioRxiv - Biophysics 2020Quote: ... in EX-CELL 293 serum-free medium (Sigma), supplemented with 3.75 mM valproic acid ...
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bioRxiv - Immunology 2020Quote: ... in EX-CELL 293 serum-free medium (Sigma), supplemented with 3.75 mM valproic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... 293 cells were transiently transfected (polyethylenimine, Sigma-Aldrich) with a mini-Gs protein and human TGR5 C-terminally fused with nano-luciferase ...
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bioRxiv - Cell Biology 2023Quote: ... and 293 cells were cultured in DMEM (Sigma). All media were supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293A cells were transiently transfected polyethylenimine (Sigma), and MEFs were transfected using GenJet (SignaGen).
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bioRxiv - Microbiology 2021Quote: ... HEK 293FT cells were cultured in DMEM (Sigma) as previously described (2) ...
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bioRxiv - Molecular Biology 2023Quote: ... into HEK-293T cells using polyethylenimine (PEI; Sigma). 48 and 72 hours later ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... EX-CELL 293 Serum-Free Medium (Sigma Aldrich, 14571C) was added to a final concentration of 20% ...
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bioRxiv - Cell Biology 2022Quote: ... RRE (Addgene 12251)24 and VSV-G (Addgene 8454)25 into AD-293 cells with PEI (Sigma-Aldrich). Two days after transfection ...
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...