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Citations for Millipore Sigma :
51 - 100 of 2111 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Digoxigenin-labelled probes were detected with anti-digoxigenin-fluorescein Fab fragments (Sigma) and Biotin-labelled probes were detected with Streptavidin-Cy5 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with anti-human Fab (Millipore Sigma) diluted 1:500 in PBS ...
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bioRxiv - Microbiology 2024Quote: ... were coated with 1/20,000 dilution of Goat anti-Human Fab (Sigma) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with anti-human Fab (Millipore Sigma) diluted 1:500 in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were detected using anti-digoxigenin antibodies with Fab fragments (Sigma-Aldrich) that were conjugated with an alkaline phosphatase (Roche ...
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bioRxiv - Neuroscience 2024Quote: Anti-DIG Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich, Cat#: 11093274910) were preabsorbed with octopus embryo powder in 1% DIG buffer in TBST for at least one hour ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated with anti-DIG Fab fragments (1:10,000) (Sigma, St. Louis, MO) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Riboprobe hybridization was detected using alkaline phosphatase-coupled anti-digoxigenin Fab fragments (Sigma). Hybridized sections were mounted in glycerol and photographed using a Nikon stereoscope and a DCC Nikon camera.
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bioRxiv - Immunology 2023Quote: ... All Fabs were buffer exchanged to TBS using 10 kDa MWCO concentrators (Millipore).
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bioRxiv - Developmental Biology 2024Quote: ... The blocking solution is exchanged with anti-Digoxigenin-AP Fab fragments (Sigma, 11093274910) diluted 1:2000 in 1% w/v lamb serum in TBST and incubated overnight at 4°C.
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bioRxiv - Immunology 2022Quote: ... Secretion of Fabs was induced using isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich), and the Fab-containing periplasmic fractions of bacteria were collected and screened by SPR using a Biacore 3000 apparatus (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... Samples were incubated with Anti-digoxigenin-AP Fab Fragment (Sigma-Aldrich, 11093274910, RRID: AB_514497) in blocking solution ≥14 hrs at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Conjugated Fabs were then concentrated using an Ultrafree 0.5 filter (10k-cut off; Millipore) to 1 mg/ml ...
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bioRxiv - Genetics 2022Quote: ... anti-Digoxigenin-AP Fab fragments was purchased from Sigma (Roche # 11 093 274 910) and used at 1:10,000 (75 mU/ml).
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bioRxiv - Neuroscience 2024Quote: ... aCSF and 192IgG-SAP groups received intraperitoneal (ip) injections of WIN55,212-2 (0.5 mg/kg or 3 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) or vehicle solution (1:1:18 ...
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bioRxiv - Plant Biology 2020Quote: ... 46 by replacing the antibody with primary anti-Digoxigenin Fab fragment (Sigma-Aldrich cat# 11214667001) and secondary donkey anti-sheep IgG (H+L ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa; Millipore) to about 1 mg ml−1 ...
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bioRxiv - Cancer Biology 2022Quote: Bi-Fabs were incubated with an excess of TCEP (20 eq, Sigma Aldrich, Gillingham, UK) to fully reduce the interchain disulfides ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa; Millipore) to >1 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction from the peak corresponding to the Fab were concentrated using Amicon Ultra-15 (Millipore) and further purified by size-exclusion chromatography using Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... or IgG control (Mouse IgG, Sigma, #I5381-1MG ...
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bioRxiv - Genomics 2022Quote: ... The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich, 11093274910) and visualized with a CDP star (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Thiolated Fab fragments were purified using 30 kDa molecular weight Amicon ultra filtration columns (Millipore, #UFC503096) and the concentration was estimated by A280 absorbance using Nanodrop 2000 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: Anti-Rabbit IgG/Anti-mouse IgG (Millipore) was used for isotype control.
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the SOSIP/Fab complexes were pooled and concentrated using 10 kDa Amicon spin concentrators (Millipore). Samples were diluted to 0.02 mg/mL in TBS (0.05 M Tris pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... The aE11-Fab sample was concentrated by centrifugal concentrator with a MWCO of 3 kDa (Merck Millipore) and further purified by size exclusion chromatography into 20 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... DIG epitopes were detected with HRP anti-DIG fab fragments at 1:2500 (Sigma-Aldrich; Reference 11207733910) and revealed using Cy3-tyramide at 1:100 ...
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bioRxiv - Immunology 2023Quote: ... The flow through containing the Fab was concentrated through 10 kDa Amicon centrifugal filter units (EMD Millipore) and buffer exchange was performed with a 7 kDa molecular weight cut-off size exclusion resin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein epitopes were detected with HRP anti-fluorescein fab fragments at 1:5000 (Sigma-Aldrich; Reference 11426346910) and revealed using Cy2-tyramide at 1:250 ...
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bioRxiv - Genetics 2023Quote: ... The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich; 11093274910) and visualized with a Bmpurple (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich; 11093274910) and visualized with a CDP star (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off, Millipore) and conjugated with CF640 or Cy3 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred into a 24-well plate filled with a FAB solution (0.7% Formaldehyde (FA, 36.5-38%, Sigma) with 0.15% or 1% acrylamide (AA ...
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bioRxiv - Immunology 2022Quote: ... Unliganded BG24S60A Fab was concentrated to 10-15 mg/mL by centrifugation with a 30-kDa concentrator (Millipore).
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bioRxiv - Cell Biology 2019Quote: ... The resulting Fabs were concentrated up to ∼1 mg/ml in PBS using a Microcon filter unit (Millipore) and labeled with NHS-ester of Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the SOSIP/Fab complexes were pooled and concentrated using 10 kDa Amicon® spin concentrators (Millipore). Samples were diluted to 0.03 mg/mL in TBS (0.05 M Tris pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing the SOSIP/Fab complexes were pooled and concentrated using 10 kDa Amicon® spin concentrators (Millipore). Samples were diluted to ∼0.03 mg/mL in TBS (0.05 M Tris pH 7.4 ...
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bioRxiv - Systems Biology 2019Quote: ... or a control IgG (Normal rabbit IgG, EMD Millipore). To collect the Immunocomplexes ...
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bioRxiv - Molecular Biology 2021Quote: ... Excess Fab was removed by ultrafiltration in TBS pH 7.4 with a 100 kDa cutoff Centricon filter (Amicon Ultra, Millipore), and the sample was concentrated to ∼5 mg/mL for cryo grid freezing ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Microbiology 2022Quote: SEC-purified LCMV pfGP-TD and LCMV pfGP-TD/M28 Fab complex in TBS was concentrated to 0.8 and 1.2mg/mL using a 30K Amicon centrifugal filter (Sigma Aldrich). For LCMV pfGP-TDapo ...
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bioRxiv - Genetics 2023Quote: ... The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Cat # 11093274910, Sigma-Aldrich, MO, USA) and visualized with a CDP star (Cat #11685627001 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated with either IgG (anti-rabbit IgG, Sigma) or GFP-nanobody (expressed from pDZ580-pET28a-GBP and purified as described in-conjugated magnetic beads (Dynabeads M-280 ...
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bioRxiv - Cell Biology 2024Quote: ... using nonspecific rabbit IgG control and mouse IgG control (Sigma). Cross-linking was reversed overnight at 65°C ...
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bioRxiv - Microbiology 2021Quote: ... IgG (Millipore Sigma), or IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... rat IgG (Sigma), rabbit F(ab’)2 anti-mouse IgG conjugated to FITC (STAR9B ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse IgG (Sigma), and rat IgG (Sigma).
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bioRxiv - Immunology 2020Quote: ... IgG (Millipore Sigma), IgG1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Rabbit IgG (Sigma) was used for the control IP ...