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Citations for Millipore Sigma :
51 - 100 of 121 citations for Colloidal Chitin polysaccharide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... a polysaccharide gel was prepared that contained (in g/100 ml) Gelrite gellan gum (2.5, Sigma), xanthan gum (0.25 ...
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bioRxiv - Microbiology 2022Quote: ... and overlaid with 10-nm colloidal gold particles (Sigma-Aldrich) for use as fiducial markers ...
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bioRxiv - Microbiology 2021Quote: ... or 8 mg ml-1 412 α-Chitin (from shrimp shells, Sigma). For each reaction ...
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bioRxiv - Microbiology 2023Quote: ... This medium was supplemented with 0.2% w/v chitin flakes (Sigma C9213) or the corresponding carbon source as indicated ...
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bioRxiv - Microbiology 2023Quote: A sample of polysaccharide 33X (1-2 mg) was dissolved in deuterium oxide (D2O) (Sigma-Aldrich, USA), subjected to centrifugation for 5 min at 16800 x g ...
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bioRxiv - Cell Biology 2021Quote: ... and subsequently stained with Brilliant Blue G Colloidal Coomassie (Sigma-Aldrich). Proteins contained in Coomassie-stained bands were digested in-gel with trypsin and identified ...
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bioRxiv - Neuroscience 2020Quote: ... a 3-μl drop of 10-nm colloidal gold (Sigma-Aldrich) was deposited on plasma-cleaned ...
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bioRxiv - Neuroscience 2023Quote: ... Protein gels were stained overnight with colloidal Coomassie Brilliant Blue (Sigma). Each lane was excised into 9 sections that were destained and in-gel digested overnight using trypsin (sequencing grade ...
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bioRxiv - Developmental Biology 2024Quote: ... and conjugated with 5nm gold nanoparticles (colloidal gold, G7277, Sigma-Aldrich) at 1:40 in 1% serum/TBST + 0.5% polyethylene glycol for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the remaining intact polysaccharide on the plates was stained with 0.2% (w/v) Congo red (Sigma Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Chitin flakes from shrimp shell was bought from Sigma-Aldrich (Catalog # C9213-500G). The flakes were firstly sieved by 2 mm or 1 mm pore size steel sieves (Amazon ...
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bioRxiv - Plant Biology 2020Quote: ... Lignin and differential polysaccharide staining were detected using a 0.02% toluidine blue O solution (Sigma-Aldrich, St. Louis, USA) as described in Methods S2.
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bioRxiv - Microbiology 2021Quote: ... and monolayers were overlaid with 1.2% microcrystalline colloidal cellulose (Sigma-Aldrich 435244) in DMEM containing 2% FBS ...
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bioRxiv - Microbiology 2024Quote: ... A 5 nm colloidal gold-conjugated antibody (diluted 1:100, Sigma, USA) was applied to the grid ...
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bioRxiv - Cell Biology 2023Quote: ... five-fold concentrated 10 nm colloidal gold (Sigma-Aldrich, St. Louis, MO) (Ivancu et al ...
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bioRxiv - Biophysics 2024Quote: ... Virus was mixed with BSA-coated 10 nm colloidal gold (Sigma-Aldrich) and 3 µL were applied to glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Plant Biology 2020Quote: ... (±)-3-Hydroxydecanoic acid (3-OH-C10:0) and chitin were obtained from Sigma-Aldrich. All elicitors were dissolved in deionized MilliQ sterile water at the respective stock concentration of 1mM for flg22Pa ...
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bioRxiv - Immunology 2022Quote: ... albicans was assessed with an end-point chitin staining with Calcofluor White (Sigma-Aldrich). Neutrophils (1 × 105 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... and a chitin-binding dye Calcofluor White (0.1 mg/ml, Sigma-Aldrich #F3543-1G) at room temperature with agitation for 2 days ...
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bioRxiv - Neuroscience 2023Quote: ... and a chitin-binding dye Calcofluor White (0.1 mg/ml, Sigma- Aldrich #F3543-1G) at room temperature with agitation for 24 h ...
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bioRxiv - Plant Biology 2023Quote: ... Chitin solution was prepared by repeatedly grinding crab or shrimp shell powder (Sigma; C9752) in water with a pestle ...
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bioRxiv - Physiology 2021Quote: ... Three replicate placental sections 80μm apart were stained for polysaccharides (including glycogen) using Periodic Acid Schiff (PAS, 395B-1KT, Sigma-Aldrich) (n = 7-11/group/sex) ...
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bioRxiv - Microbiology 2024Quote: Co-cultures were mixed with 10 nm colloidal gold beads (Sigma-Aldrich, Australia), which had been precoated with 1% BSA ...
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bioRxiv - Microbiology 2020Quote: ... following staining of cell wall chitin with 25 μM calcofluor white (Sigma, catalog number 18909) in PBS for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Chitin precipitate was then placed in cellulose dialysis tubing (approximately 13kDa, Sigma-Aldrich D9652-100FT) and dialyzed with fresh deionized water daily for three days to remove residual phosphoric acid and oligomers ...
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bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg/ml of calcofluor white (to visualise chitin; 18909, Sigma-Aldrich, St. Louis, United States), or 500 μl/ml of aniline blue (to visualise β-glucans ...
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bioRxiv - Microbiology 2024Quote: ... These mixtures were then incubated in seawater (collected from Jinshan, Shanghai) supplemented with 0.5% chitin (Sigma) for 72 hours at 30°C under static conditions ...
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bioRxiv - Microbiology 2024Quote: A standard curve for Alcian Blue labeling of acid polysaccharides was prepared with Xanthan Gum (“XG”; Sigma-Aldrich G1253, ordered 10/2022) using the updated method of Bittar et al ...
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bioRxiv - Molecular Biology 2021Quote: ... Chitin in the ASG was labeled using FITC conjugated wheat germ agglutinin (FITC-WGA, Sigma-Aldrich, USA).
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bioRxiv - Cancer Biology 2020Quote: ... The pH of 1000 μl 20nm colloidal spherical monodispersed citrate stabilized gold nanoparticles (Sigma Aldrich) was adjusted to a pH unit of 0.5 higher than the iso-electric point (IEP ...
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bioRxiv - Cell Biology 2021Quote: ... with a 3:1 concentrated solution of 20 nm:5 nm colloidal gold (Sigma Aldrich) blocked with bovine serum albumin ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were incubated with 10-nm colloidal gold-conjugated secondary antibody (Sigma, Cat G7402). The exosomes were stained with 2% uranyl acetate for 3 minutes before observation using a JEM 1400 plus transmission electron microscope at 120 kV.
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bioRxiv - Immunology 2022Quote: ... After that 50 µl of 1:5 dilution Protein A colloidal gold conjugate (Sigma, USA), which is used as an indicator was added to the test hole and was allowed to soak for 2 minutes ...
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bioRxiv - Microbiology 2022Quote: ... pastoris was adjusted to a concentration of 5μg/ml (in 800μl) and incubated with 3mg of chitin from crab shell (Sigma), chitosan (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived secondary antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963), the goat-derived secondary antibody (anti-mouse ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated with 10 nm colloidal gold-labeled anti-mouse IgG secondary antibody (G7652, Sigma-Aldrich) diluted 1:25 for 3 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived second antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963) is available as a commercial product.
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bioRxiv - Molecular Biology 2022Quote: ... The gels were stained with Coomassie Brilliant Blue G-250 colloidal (EZBlue Gel Staining Reagent, Sigma). After washing with water ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with goat anti-rabbit antibody labeled with 10 nm colloidal gold particles (Sigma-Aldrich) diluted 1:10 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Gel slices corresponding to each sample lane were excised and stained with Coomassie colloidal blue (Sigma). All steps from in-gel digestion and mass spectrometry data acquisition were carried out at the mass spectrometry platform of the Carlos Chagas Institute ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 μM octa-N-acetyl-chitooctaose (CO8, obtained from Sébastien Fort28) or 0.1 mg/mL shrimp-cell chitin (Sigma Aldrich). Luminescence was recorded with a Varioskan LUX multimode microplate reader (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 3.1 μL of sample was mixed with 1 μL of 10 nm colloidal gold beads (Sigma-Aldrich) as fiducial markers and then applied to 300 mesh Quantifoil R2/2 grids ...
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bioRxiv - Neuroscience 2023Quote: ... and 9–11 nm colloidal gold conjugated to goat anti mouse IgG secondary antibodies (Sigma-Aldrich, #G7652). Following immunogold labeling ...
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bioRxiv - Microbiology 2023Quote: ... Overnight cultures were then used to inoculate M9 medium supplemented with 5% (wt/vol) chitin from shrimp shells (Sigma-Aldrich) and grown at 18 °C for 48-72 h ...
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bioRxiv - Biochemistry 2022Quote: ... Elution from the chitin beads was done by overnight incubation with 50 mM of 2-mercaptoethanesulfonic acid (MESNA) (Sigma Aldrich) that induces intein-mediated cleavage ...
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bioRxiv - Biochemistry 2022Quote: ... 250 mM sucrose and mixed in a 1:1 ratio with 5-nm colloidal gold solution (Sigma Aldrich) and vitrified as described above on glow-discharged Quantifoil R2/2 Au 200 mesh grids ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were then analysed by Coomassie staining using Brilliant Blue G colloidal concentrate (Sigma Aldrich, B2025) on 4–12% NuPAGE Bis-Tris gels (Life Technologies).
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bioRxiv - Microbiology 2022Quote: ... and the cell monolayers were washed twice with PBS and then overlaid with 1% colloidal microcrystalline cellulose (Sigma) in MEM containing 2% FBS ...
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bioRxiv - Biophysics 2024Quote: ... The instrument response function was collected by measuring scattered light from a colloidal suspension of Ludox (Sigma Aldrich). The lifetime decays were fit to a sum of exponentials using the following equation: where I(t ...