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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Aspartate Aminotransferase Cytoplasmic GOT1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 1.5% uranyl acetate (Serva) in H2O at 53°C for 2h and 0.02M lead-aspartate (Sigma-Aldrich) at 53°C for 2h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... anti-phosphorylated (Ser897)-N-methyl-D-aspartate receptor (anti phosphoNMDAR cat.#ABN99, Merck Millipore, Burlington, MA, USA), anti-total-calcium/calmodulin-dependent protein kinase II (anti-tCaMK ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µL cytoplasmic fraction was dropped onto nylon membrane (Sigma, 15356) to immunoblotting ...
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bioRxiv - Immunology 2019Quote: ... Nuclear and cytoplasmic fractions were isolated using NucBuster (Novagen 71183-3). If appropriate ...
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bioRxiv - Physiology 2023Quote: ... a colorimetric-based enzyme assay was used to measure alanine aminotransferase (ALT) levels in peripheral plasma collected from the submandibular vein monthly using a Mouse ALT Activity Assay Kit (SIGMA). Serum LBP level was measured by a Mouse Lipopolysaccharide Binding Protein ELISA Kit (HyCult Biotechnology ...
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bioRxiv - Immunology 2023Quote: The serum alanine aminotransferase (ALT) activity was analysed using a commercially available kit according to the manufacturer’s guidance (Sigma-Aldrich) and a Spark multimode microplate reader (Tecan ...
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bioRxiv - Plant Biology 2022Quote: ... The lead aspartate was prepared with 0.04 g L-aspartic acid (#a9256; Sigma-Aldrich, St Louis, MO, USA) and 0.066 g lead nitrate (#203580-10G ...
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bioRxiv - Neuroscience 2024Quote: ... neurotoxic lesions in ACC were made by infusing N- methyl-D-aspartate (NMDA, Sigma-Aldrich, St. Louis, MO) in a phosphate-buffered saline (PBS ...
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bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... and 50 μM for the cytoplasmic dynein inhibitor Ciliobrevin D (Sigma Aldrich). For the live cell imaging experiments drugs were added at the same moment as cells were seeded to chambers ...
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bioRxiv - Immunology 2023Quote: ... and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108, Sigma Aldrich). 2 Million cells were injected intravenously into mice two hours prior to imaging ...
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bioRxiv - Neuroscience 2022Quote: Lesion animals (n=18) received bilateral excitotoxic HPCv lesions via intracranial injections of N-methyl-d-aspartate (NMDA, Sigma, St-Louis ...
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bioRxiv - Cell Biology 2019Quote: Cytoplasmic ROS was quantified using 2,7-dichlorofluorescin-diacetate (H2-DCFDA) dye (Sigma-Aldrich) modified from (Artal-Sanz and Tavernarakis ...
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bioRxiv - Molecular Biology 2020Quote: ... as cytoplasmic marker and U1-70k (EMD Millipore, Cat.# 05-1588, 1:1,000) as nuclear marker.
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bioRxiv - Developmental Biology 2020Quote: ... The cytoplasmic fractions were concentrated using AmiconUltra – 0.5mL – 30K centrifugal filters (Merck Millipore) and mixed 1:1 with 2X Laemmli sample buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Colorimetric assay to measure alanine aminotransferase activity in the cell lines was performed using the ALT activity assay kit from Sigma (MAK052-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The gradient was fractionated into 200 μl fractions and the crosslinking reaction was quenched using 100 mM aspartate (Sigma Aldrich) at pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Systems Biology 2021Quote: The method for separation of nuclei and cytoplasmic fractions was adapted from Sigma-Aldrich single nuclei isolation protocol (nuclei EZ prep nuclei isolation kit ...
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bioRxiv - Cancer Biology 2021Quote: ... The cytoplasmic fraction was concentrated using Amicon Ultra 0.5 mL Centrifugal Filters (Sigma-Aldrich), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cytoplasmic RNA was purified from the supernatants using 1 ml TRI Reagent (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Mel-526 cells stained with BioTracker 490 green cytoplasmic membrane fluorescent dye (Millipore Sigma) were seeded on poly-L Lysine pretreated 96 well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from the cytoplasmic and nucleoplasmic fractions using TRIzol reagent (Sigma-Aldrich), according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Semaphorin 6B cytoplasmic tail (aa 616-1021) (SEMA6B, amplified from human brain cDNA library, Novagen); Semaphorin 6D cytoplasmic tail (aa 684-1073 ...
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bioRxiv - Cell Biology 2021Quote: ... cytoplasmic dynein (dynarrestin, 50 μM; Sigma-Aldrich and ciliobrevin A, 10 μM; R&D Systems); and β−tubulin (nocodazole ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from the cytoplasmic and nucleoplasmic fractions using TRIzol reagent (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... a sharp glass pipette (tip inner diameter: 15–30 μm) filled with NMDA (N-methyl-D-aspartate, Sigma-Aldrich, M3262, 0.2 mM), C21 (200 µg/ml ...
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bioRxiv - Immunology 2024Quote: Aspartate (Scientific Laboratory supplies, CHE2306) was dissolved in iron free media by slowly titrating in 1M NaOH (Sigma Aldrich, 30620-1KG-M) until aspartate was completely solubilised and the pH ∼7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts of cytoplasmic and nuclear cell fractions were prepared using the CelLytic NuCLEAR Extraction Kit (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... were transduced to stably express cytoplasmic fluorescence (LentiBriteTM GFP Control Lentiviral Biosensor 17-10387, Millipore Sigma) and cultured in Vasculife Endothelial Medium (LL-0003 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration for the total and cytoplasmic lysates were quantified by Bradford Reagent (B6916, Sigma Aldrich) and 10-20 μg of protein was used for western blot experiments ...
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bioRxiv - Bioengineering 2024Quote: ... HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor, Millipore Sigma Aldrich), and were used between passages 6 and 9 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant (cytoplasmic fraction) was collected and concentrated using Amicon Ultracel-10 centrifugal filter units (Millipore). 20% of mitochondrial and 10% of cytoplasmic fractions were then loaded in SDS-PAGE and analyzed by western blotting ...
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bioRxiv - Bioengineering 2023Quote: ... HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor, Millipore Sigma Aldrich), and were used between passages 6 and 9 ...
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bioRxiv - Cell Biology 2023Quote: ... then nuclei and cytoplasmic fractions separated using CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich, NXTRACT-1KT). The resulting protein was run on a western blot and normalized to loading controls Histone H3 for nuclear fraction and alpha-tubulin for cytosolic (Sup ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were then subjected to inter-cytoplasmic microinjection in M2 medium covered with mineral oil (Sigma, BioXtra) at r.t ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... HDLMEC were transduced to express cytoplasmic RFP using LentiBrite™ RFP Control Lentiviral Biosensor (EMD Millipore, 17-10409) as described by the vendor ...
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bioRxiv - Microbiology 2020Quote: Nuclear and cytoplasmic extracts were prepared from differentiated THP-1 cells using Nuclei EZ Prep kit (Sigma-NUC101) both from un-stimulated and MprA stimulated cells ...
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bioRxiv - Biophysics 2021Quote: ... Gelsolin TL40 (N-terminal cytoplasmic gelsolin, Hypermol, Germany) was covalently bound to carboxylated microspheres (0.9 µm diameter, Sigma Aldrich) with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in order to bind the +end of a single actin filament ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the nuclear/cytoplasmic cell fractionation Cell Lytic™ NuCLEAR™ extraction kit were from Sigma (St. Louis, MO). Sequencing grade trypsin was purchased from Promega (Madison ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality of nuclear and cytoplasmic extraction was validated by western blotting by α-Histone 3 (Sigma, St. Louis, MO) (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiac tissues were fractionated into nucleus-enriched and cytoplasmic samples by using a CelLytic™ NuCLEAR Extraction kit (Sigma, Nxtract), as previously described (32).
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant as cytoplasmic fraction was collected and concentrated with a Centricon column (10-kDa cutoff, Millipore-Sigma-Aldrich). The pellet as nuclear fraction was lysed with a lysis buffer including 0.1 N NaOH and 0.1 % SDS.
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant as cytoplasmic fraction was collected and concentrated with a Centricon column (10-kDa cutoff, Millipore-Sigma-Aldrich). The pellet as nuclear fraction was lysed with a lysis buffer including 0.1 N NaOH and 0.1 % SDS.
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bioRxiv - Immunology 2024Quote: ... Cytoplasmic protein extracts were obtained by incubation of cells with lysis buffer [50 mM NaH2PO4 (Sigma-Aldrich, Cat. No. S8282), 200 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Preparation of nuclear and cytoplasmic fractions was performed according to the user manual supplied with the CelLytic™ PN Plant Nuclei Isolation/Extraction Kit (Sigma) with minor modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and precleared with protein-sepharose beads for 1 h at room temperature ...