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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... followed by the incubation with extravidin-peroxidase (1:1000, Sigma- Aldrich Cat# e2886, RRID:AB_2620165). The antigen-antibody binding is then detected using chromogen 3,30-diaminobenzidine (DAB ...
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Neuronal connectivity, behavioral, and transcriptional alterations associated with the loss of MARK2bioRxiv - Neuroscience 2023Quote: ... and 10 mM DTT and supplemented with protease inhibitor cocktail (1:1000, Sigma Aldrich), phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Biophysics 2023Quote: ... 0.5% NP-40 alternative) containing protease inhibitors-1x aprotinin (Sigma, #A6279, diluted 1:1000), 1 mM benzamidine (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... cleaved Caspase-3 Asp175 (5A1E #96641:1000) and anti-FLAG M2 antibody (Sigma, #F1804). HRP-conjugated secondary antibody was used at a dilution of 1:5000 (Jackson Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... we also used clone CON.1 (1:1000 for IF) from Sigma-Aldrich (#c1985). Mouse monoclonal anti-AP2 (1:100 for IF ...
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bioRxiv - Neuroscience 2023Quote: ... IgG1 isotype control-AlexaFluor 488 (1:1000, Mouse monoclonal IgG1-k, FCMAB310A4; Millipore, MA) and DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... and mouse monoclonal anti-FLAG® M2 (Cat# F1804-50UG, Sigma Aldrich, 1:1000).
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies donkey anti mouse conjugated with CFTM 555 (1:1000, Sigma-Aldrich SAB4600060) and donkey anti rabbit conjugated with DyLight 650 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 1000 units/ml DNase-1 and 5 mg/ml glucose (all from Sigma Aldrich) in Hank’s basal salt solution medium (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the following primary antibodies: anti-flag HRP-conjugated (1:1000; Sigma-Aldrich, A8592), anti-αTubulin (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... 5) 30 seconds in a 1:1000 dilution of 28% Ammonium Hydroxide (Sigma 338818) in mQ water ...
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bioRxiv - Neuroscience 2024Quote: ... Floating sections were stained with rabbit anti TH antibody (AB152,1:1000, Millipore SIGMA, USA), and mouse anti PHOX2B (B-11 ...
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bioRxiv - Cancer Biology 2024Quote: ... for nuclear co-staining a 1:1000 dilution of Hoechst (Millipore-Sigma, Cat#33258) was added to the solution ...
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bioRxiv - Cancer Biology 2024Quote: ... for nuclear co-staining a 1:1000 dilution of Hoechst (Millipore-Sigma, Cat#33258) was added to the solution ...
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bioRxiv - Bioengineering 2024Quote: ... and PEG-dithiol (DT, Mn = 1000, Sigma catalog #717142; Mn = 3000, Sigma catalog #704539). Cell culture reagents were purchased from Fisher Scientific unless otherwise noted.
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in 0.3% PBST and incubated with DAPI (Millipore, D6210, 1:1000) for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... or the microtubule depolymerization inhibitor Taxol (1000 µM for 1 hour; PHL89806, Sigma-Aldrich). Vehicle treatment of DMSO (D2650 ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse anti-UBE3A (E6AP; WB: 1:1000; IF: 1:200; Sigma-Aldrich; Cat# E8655). Mouse anti-Ubiquitin (WB ...
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bioRxiv - Microbiology 2024Quote: ... T was prepared in 20% (wt/wt) d-α tocopheryl polyethylene glycol 1000 (Sigma) succinate solution ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody used was a mouse monoclonal anti-NeuN (1:1000; Millipore MAB377), and the secondary antibody used was a goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... followed by a secondary HRP-conjugated donkey anti-chicken antibody (1:1000) (Merck Millipore). The western blots were analysed with Clarity Western ECL substrate (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... 150,000 cells were stained with 10 μM 4’-(trimethylammonio)-diphenylhexatriene (TMA-DPH) or 2 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine; both obtained from Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... and mixed with 100 μL of 2× GPCR solution ligand (2 μM neurotensin for FLAG-NTSR1 or 20 μM Isoproterenol (Sigma-Aldrich) for FLAG-β2AR) ...
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells with doxycycline-inducible short hairpin RNA-micro RNA (shRNA – Supplementary Table 2) sequences targeting Tet1 or Tet3 were treated for 48h with 2 μg/mL doxycycline (D9891, Sigma Aldrich).
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bioRxiv - Cancer Biology 2019Quote: ... injected daily with 5 mg/kg body weight ethyl-2-[6-(4-chlorophenoxy)hexyl]-oxirane-2-carboxylate (etomoxir) (Sigma-Aldrich, US), dissolved in 5% (2-Hydroxypropyl)-β-cyclodextrin solution from day 8 following tumor transplantation (LLC-Eto) ...
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bioRxiv - Molecular Biology 2019Quote: ... then inoculated in required media at 2×104 cells/ml and allowed to recover for 2 hours at 30° before addition of 1μM β-estradiol (Sigma E2758) and CuSO4 as required ...
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bioRxiv - Cell Biology 2019Quote: ... 4,000 cells per cm2 in passage 2 were seeded on a 12 well plate coated with fibronectin (2 μg/cm2; Sigma Aldrich) and allowed to grow confluent ...
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bioRxiv - Microbiology 2019Quote: ... in brain heart infusion medium supplemented with 2% yeast extract (BHIS; Βecton Dickinson Company) or Mueller Hinton Broth (MHB; Difco) with 2 μg/mL thiamphenicol (Sigma-Aldrich), 3.125 – 60 μg/mL cefoperazone (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice with PBS1X (5 mM EDTA, 2% fetal bovine serum (FBS)) before being fixed in 2% formaldehyde solution (252549, Sigma-Aldrich). After incubation at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cysteine thiols were subsequently reduced and alkylated by adding Tris(2-carboxyethyl)phospin (Carl Roth) to a final concentration of 10 mM and 2-Chloroacetamide (Sigma-Aldrich) to a final concentration of 40 mM ...
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bioRxiv - Biophysics 2021Quote: ... Cardiomyocytes were attached to imaging chambers (laminin-coated coverslips attached to acrylic slides) for 2 hours at 37°C and fixed in 2% paraformaldehyde (Sigma-Aldrich) (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich) and 4% BSA (Bovine Serum Albumin ...
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bioRxiv - Plant Biology 2020Quote: Stock solutions of 60 mM PAO (Phenylarsine oxide) in DMSO and 50 mM 2-BP (2-bromopalmitate) in EtOH were ordered from Sigma-Aldrich. Seven-day-old seedling grown in vitro vertically (root tip analyses) ...
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Plant Biology 2021Quote: ... Five days after germination the plant were incubated in liquid in ½ MS with 1% sucrose media with or without 50 µM 2-bromopalmitate (2-BrP; Sigma-Aldrich), and with or without 100 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Mice were kept on 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. No. PHR1731-1G) in drinking water when indicated ...
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bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... PLGA-PEI NPs were dispersed in 100 mM phosphate buffer with EDTA (2 mM) (pH = 8.0) and mixed with excess amount of 2-iminothiolane (Traut’s reagent, Sigma-Aldrich, Cat# I6256). After continuous rotation overnight at RT ...
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bioRxiv - Neuroscience 2022Quote: ... participants were administered a dosage of 2 g tyrosine (supplied by The Hut.com Ltd.) or placebo (2 g cellulose powder (Sigma-Aldrich Co.), solved in 200 ml orange juice (Solevita ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Neuroscience 2020Quote: ... the mice were anesthetized with 20 mg/kg 1.25% Avertin (a mixture of 12.5 mg/mL of 2,2,2-Tribromoethanol and 25 μL/mL 2-Methyl-2-butanol, Sigma, St. Louis, MO, USA) through intraperitoneal injection and then placed in a prone position in the stereotaxic frame ...
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bioRxiv - Immunology 2020Quote: ... Cells are washed in an excess of HBSS + 2%FBS and are resuspended in HBSS + 2%FBS with DAPI (Sigma-Aldrich) to discriminate live from dead cells.
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bioRxiv - Neuroscience 2022Quote: ... 1:400) for 2-h at room temperature and then stained with 4,6-diamidino-2-phenylindole dihydrochloride (DAPI, #D9542, Sigma, Darmstadt, Germany) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The total volume was concentrated to ∼2 ml by spin filtration using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane (Sigma-Aldrich). The non-vesicular protein fraction volumes were each collected in 2 ml of PBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Supernatants were discarded and pellets was re-suspended in 2 ml wash buffer (50 mM Tris pH6.8, 2 mM MgSO4, 10 U/ml Benzonase (Sigma-Aldrich, Germany)) and incubated an additional 20min on ice to degrade genomic DNA ...
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bioRxiv - Systems Biology 2020Quote: BALB/cJ mice were subjected to cutaneous Oxazolone (Oxa) challenge by applying 5% 4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in acetone and olive oil topical to the skin as described [74] ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated with frequent agitation at 37°C for 20 min in 2 ml of 2 mg/ml papain (Sigma, P4762) solution in Hibernate-A ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...