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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... and both fractions were checked by SDS-PAGE followed by staining with Coomassie-based dye according to the manufacturer’s instructions (Instant Blue Protein Stain, Sigma ISB1L-1L).
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P3HT-GRAPHENE DEVICE FOR THE RESTORATION OF VISUAL PROPERTIES IN A RAT MODEL OF RETINITIS PIGMENTOSAbioRxiv - Neuroscience 2022Quote: ... ionized calcium-binding adaptor molecule 1 (Iba-1, 1:1000; Wako Pure Chemical Industries, Japan) and glial fibrillary acidic protein (GFAP, 1:200; Sigma-Aldrich) in 1% NGS and 0.3% Triton-X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Physiology 2022Quote: ... propionate and butyrate in the serum was determined by ion chromatography analysis after depletion of proteins and lipids with acetonitrile (Sigma-Aldrich). Samples were loaded on a Dionex ICS-5000 Plus system automatic device (ThermoScientific ...
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bioRxiv - Cell Biology 2024Quote: ... Equal concentration of total protein extracts were incubated with Myc (#1-9E10.2 hybridoma supernatant, ATCC, USA) or HA (#11583816001, 12CA5, Sigma-Aldrich, USA) antibodies for 2h at 4°C on a rotator ...
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bioRxiv - Cell Biology 2024Quote: ... A pair of gRNAs with minimum off-target and maximum on-target activity were designed and selected to specifically target sequences that encode essential protein domains of the Bend2 gene using CRISPR DESIGN TOOLS (Millipore Sigma) (gRNA1-antisense ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates from cell lines were prepared using the CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, St. Louis, MO, USA), while CelLytic™ MT Mammalian Tissue Lysis Reagent (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The protein-containing fractions were pooled and concentrated in an Amicon spin concentrator of 10-30kDa cutoff (Millipore, UCF901024 and UCF903024) and buffer exchanged against Buffer B (20 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... isolated mitochondria equivalent to 1 mg protein were mixed with 250 μl methanol before adding 5 mL 0.1M Hydrochloric acid (Sigma, Cat# 1090601000) and 100 µL SPLASH (Avanti Polar Lipids ...
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bioRxiv - Neuroscience 2024Quote: ... For LC-MS/MS analysis protein gels were stained over night at room temperature with Coomassie solution (B8522-1EA; Sigma-Aldrich) and washed with 2.5% acetic acid and 20% methanol and subsequent washes of 20% methanol ...
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bioRxiv - Plant Biology 2024Quote: ... Active PME units per µg of protein were calculated by using a standard curve generated with known amounts of commercial PME (Sigma, P5400).
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bioRxiv - Developmental Biology 2024Quote: ... 1 nL of 12.5 ng/µL of sgRNA and 1 nL of Cas9 protein (300 ng/µL)64 along with phenol red (Sigma Aldrich) were co-injected in one-cell stage embryos ...
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bioRxiv - Cell Biology 2024Quote: ... Equal quantities of proteins (20 μg/lane) were loaded onto 8% or 10% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore, USA). The membranes were then incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The eluted fractions from solution C (containing target proteins) were applied to an Amicon Ultra 15 Centrifugal Filter (Millipore Billerica MA) for desalting and imidazole removal by using a buffer containing 100 mM Tris–HCl (pH 7.9) ...
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bioRxiv - Microbiology 2024Quote: ... correct production of the protein of interest was checked by Western Blot of HEK293T-transfected cells using an anti-Flag antibody (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... 1 μl of serum sample (∼70-80 μg protein) was lysed with 0.1% Rapigest (Waters, MA, USA) in 100 mM ammonium bicarbonate (Sigma, MO, USA) and denatured at 95°C for 5 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein quantification was performed using the BCA protein quantification assay (QPro BCA kit, Cyanagen) against a bovine serum albumin (BSA) calibration curve (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Expression of the different FLAG-SET8 proteins in U2OS cells were induced by the addition of 2 µg/ml of tetracyclin (TET) (Sigma-Aldrich) into cell culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins eluted by 100 mM imidazole were collected and then desalted with disposable PD 10 desalting columns (Sigma, GE17-0851-01) against stocking buffer (20 mM HEPES (pH 7.3) ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound samples were successively incubated with appropriate amount of primary antibodies against protein-of-interest (CTCF antibody: Millipore, 07-729) in 50µL of CUT&RUN antibody buffer for overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... Equal amounts of protein were separated on a SDS-PAGE gel and transferred to an Immobilon-P polyvinylidene difluoride (PDVF) membrane (Millipore, IPVH00010). Membranes were blocked in PBS with 3% milk powder (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-AGO1 proteins were detected with anti-FLAG-HRP (1:4000 [Sigma] in 5 % milk in PBS-T [0.05% tween20]) or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were incubated overnight at 4 °C in a humidified chamber: mouse anti-glial fibrillary acidic protein (GFAP) (1:500; Sigma-Aldrich), rabbit anti- laminin 1α (1:200 ...
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bioRxiv - Immunology 2022Quote: ... 1 μl of serum sample (∼70-80 μg protein) was lysed with 0.1% Rapigest (Waters, MA, USA) in 100 mM ammonium bicarbonate (Sigma, MO, USA) followed by denaturation at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Sheared chromatin equivalent to 2 × 106 cells was diluted 1:10 in ChIP Dilution Buffer and protease inhibitor cocktail and incubated for 16 h with 20 μL Magnetic Protein A/G Beads (Millipore #CS204457) and 5 μL antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Immunology 2022Quote: The nuclear protein extracts from the TNF-stimulated A549 cells were subjected to SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, USA) using the transfer buffer (192 mM glycine ...
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bioRxiv - Molecular Biology 2023Quote: ... the remainder was pre-cleared by co-incubation with 40 µL of PureProteome Protein A/G magnetic beads (Merck Millipore, LSKMAGAG10) per sample and rotation for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich, I1284). Bacteria pellets were resuspended in Bugbuster (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Isolated adipocytes obtained as described above were washed in PBS three times and proteins were extracted from 500 μl of adipocyte with 500 μl 1X RIPA buffer (Sigma-Aldrich) supplemented with complete antiprotease tablet (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... briggsae ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 400 μl with (10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Physiology 2023Quote: ... The resulting crude mitochondrial pellet fraction was resuspended in MIB and protein concentration was quantified with Bradford assay (Sigma-Aldrich, B6916) using a spectrophotometer (Infinite 200 Pro ...
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bioRxiv - Biochemistry 2023Quote: ... Reagents for protein chemistry (e.g., iodoacetamide, dithiothreitol, guanidine hydrochloride, EDTA, and formic acid) were purchased from Sigma Aldrich (St. Louis, MO). Proteomics grade trypsin was from Promega (Madison WI) ...
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bioRxiv - Molecular Biology 2023Quote: The dox-inducible A375P cells were treated with doxycycline to express MITF WT and MITF mutant proteins for 24 hours and then treated with 40 µg/ml cycloheximide (Sigma #66819), in presence or absence of 200nM TPA ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1 mL using a 50 kDa molecular weight cut-off (MWCO) centrifugal filter (Millipore, #UFC505024). Finally ...
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bioRxiv - Biophysics 2023Quote: ... The purified proteins were then buffer-exchanged into 20 mM CAPS pH 11 storage buffer using 3K Amicon Ultra centrifugal filters (Millipore, USA). Small aliquots of the protein were frozen in liquid nitrogen at a protein concentration of approximately 1 mM and stored at -80 °C.
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bioRxiv - Bioengineering 2023Quote: The purified proteins were concentrated to a concentration of 20 mg/mL using a 10 kDa Amicon® tube (Millipore, UFC9010), and the buffer was exchanged to PBS ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... The peroxidase activity of 3-Amino-9-ethylcarbazole (AEC) was used to visualize the protein expression and the sections were mounted using glycerol gelatin (Sigma-Aldrich). See Table 5 for details on the antibodies used for immunofluorescence ...
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bioRxiv - Developmental Biology 2023Quote: ... All proteins were separated in 5-22% gradient SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, Billerica, MA) using a semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Biophysics 2023Quote: ... The purified proteins were then buffer-exchanged into 20 mM CAPS pH 11 storage buffer using 3K Amicon Ultra centrifugal filters (Millipore, USA). Small aliquots of the protein were frozen in liquid nitrogen at a protein concentration of approximately 1 mM.
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bioRxiv - Biochemistry 2023Quote: ... Proteins were snap-frozen in small aliquots after concentrating them in this buffer using a Centricon 50 (EMD Millipore, Billerica, MA). Protein concentrations were determined using BSA standards on a Coomassie-Blue-stained SDS-PAGE gel.
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...