Labshake search
Citations for Millipore Sigma :
901 - 950 of 3646 citations for Recombinant Human S100A6 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
-
bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
-
bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
-
bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
-
bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were immune precipitated for 2.5 hours at 4°C using Ezview anti-HA agarose (Sigma-Aldrich) as described above ...
-
bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
-
bioRxiv - Genomics 2022Quote: ... SPA-tagged protein was pulled down from clarified lysates with an anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) bound to Protein A-Sepharose beads (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... and analyzed by Western blotting for detection of 3xFLAG-tagged IsdF using an anti-FLAG M2 antibody (Sigma, #F3165) and infrared imaging (Odyssey CLx ...
-
bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins from cleared lysates was performed with Anti-Flag M2 Affinity Agarose Gel (Sigma-Aldrich) at 4°C for 3 h ...
-
bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
-
bioRxiv - Biochemistry 2023Quote: ... The FLAG-tagged RIG-I was pulled down using anti-FLAG M2 magnetic beads purchased from Sigma-Aldrich (M8823). The proteins were eluted from beads in 30 μL of 4x Laemmli buffer heated to 95 °C for 5 mins and the entire elution was run on a Western blot using anti-FLAG (Sigma-Aldrich F3165 ...
-
bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged proteins were detected by alkaline-phosphatase-conjugated anti-FLAG IgG (A9469, Sigma Aldrich, St. Louis, MO) and 1-Step NBT/BCIP substrate solution (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... 3FLAG-6HIS-YFP-tagged proteins expressed from the endogenous locus were immunoprecipitated using anti-FLAG M2 antibodies (F3165, Sigma) (Unnikrishnan et al. ...
-
bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
-
bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
-
bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
-
bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
-
bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
-
bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... which contained soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
-
bioRxiv - Biochemistry 2024Quote: ... which containing soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
-
bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
-
bioRxiv - Microbiology 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated using a 30K MWCO concentrator (EMD Millipore) to 0.2 ml and diluted to 0.5 ml in GF150 buffer (25 mM HEPES [pH7.4] ...
-
bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
-
bioRxiv - Genetics 2022Quote: ... Human Erythroleukemia (HEL) and human TF-1 cells were cultured in RPMI-1640 medium (Sigma Aldrich #R8758) supplemented with 10% Fetal Bovine Serum (Sigma Aldrich #F0926 ...
-
bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
-
bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
-
bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
-
bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
-
bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
-
bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
-
bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
-
bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
-
bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
-
bioRxiv - Biochemistry 2019Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
-
bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were clarified by centrifugation before GFP-FLAG-tagged proteins were isolated by incubation with 100 µL packed FLAG M2 affinity resin (Sigma) for 1 h with rotation at 4°C ...
-
bioRxiv - Microbiology 2021Quote: Samples of episomal PPS-GFP Dd2 or endogenously HA-FLAG-tagged PPS Dd2 parasites were harvested by centrifugation and treated with 0.05% saponin (Sigma 84510) in PBS for 5 min at room temperature and spun down by centrifuge at 5,000 rpm for 30 minutes at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... The resin was loaded into a column and the flow through containing S-tagged gyrase A was further purified using an S-protein agarose (Sigma) by elution with 20 mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were hybridized overnight at 37°C with sixteen FLAP-Y-Cy-3 tagged complementary oligonucleotide probes, targeting regions in Cox7c mRNA (IDT) in Hybridization mix (15 % Formamide, 1.7 % tRNA (Sigma; R1753), 2 % FLAP:Probe mix ...
-
bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
-
bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were lysed with whole-cell extract buffer and the FLAG tagged proteins were immunoprecipitated overnight at 4°C using anti-FLAG-agarose beads (Sigma), as described above ...
-
bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
-
Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).