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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... We biotinylated fetuin by combining 1.5ml fetuin (Sigma Aldrich) at 25 mg.ml-1 with 127 µl of 100nM Biotin NHS-LC (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated HIF1α was purified with streptavidin agarose beads (Sigma) and precipitates analysed by immunoblot analysis[19].
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bioRxiv - Immunology 2024Quote: ... and enzymatically biotinylated using BirA ligase (Sigma-Aldrich #CS0008) overnight at 4°C according to the manufacturer’s protocol (BirA ...
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bioRxiv - Microbiology 2021Quote: ... and recombinant chondriotinase ABC (Sigma, C3667) was treated at 25 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant active AMPK (Millipore, 14-840) was added and incubated for 30 min at 30°C ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant IFNα was purchased from Sigma (Interferon-αA/D human Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... and Recombinant mouse LIF (Sigma, #ESG1107). Additionally ...
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bioRxiv - Immunology 2021Quote: ... Recombinant purified Angiotensin II (Sigma – A9525) at 1 μM with 2-fold serial dilutions ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant leukemia inhibitory factor (SRP3316; Sigma), growth hormone (869008 ...
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bioRxiv - Neuroscience 2023Quote: ... and/or recombinant Fibronectin (Sigma-Aldrich) were applied as described using a range of concentrations (μg/ml).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Cell Biology 2024Quote: ... The recombinant laminin (L6274, Sigma‒Aldrich) we used was almost identical to Biolaminin 511 LN because nearly all the laminins in L6274 were laminin-511 (Wondimu et al. ...
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bioRxiv - Bioengineering 2024Quote: ... 100 mIU/mL recombinant hCG (Sigma), 500 ng/mL of androstenedione (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... and purified using DDDDK-tagged protein purification gel (MBL, Japan) followed by elution with 0.1 mg/ml FLAG Peptide (Sigma-Aldrich Co., St. Louis, Missouri, the US). To remove the flag peptide ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... and then stained with biotinylated secondary antibody (SAB4600042, Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... biotinylated probes shown in Figure S3 were synthesized (Sigma, U.S.A) and annealed to a final concentration of 25 pmol/μL ...
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bioRxiv - Microbiology 2020Quote: ... or biotinylated anti FLAG antibody (Sigma Aldrich, Cat. No. F9291) for detection of preys ...
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bioRxiv - Immunology 2020Quote: ... coupling with biotinylated-bovine serum albumin (100 μg/ml, Sigma) then neutravidin (10μg/ml) ...
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bioRxiv - Biochemistry 2022Quote: ... Biotinylated UNC-6ΔC or heparin (Millipore Sigma, cat. no. 375054) was captured on a streptavidin-coated sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... The channel was first incubated with biotinylated-BSA (Sigma Aldrich)) solution (1mg/mL biotinylated-BSA in Buffer A (10mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Systems Biology 2019Quote: ... and un-tagged CARD14 was detected with anti-CARD14 (HPA023388, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... The normalized supernatants were mixed with FLAG-tagged beads (Sigma, A2220) at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... a C-terminal 6His-tagged version was cloned onto pET22b (Novagen) for expression in E ...