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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and 5-fluorouacil (5-FU; Sigma-Aldrich) were dissolved in DMSO ...
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bioRxiv - Physiology 2024Quote: ... 5-hydroxytryptamine (5-HT, serotonin) (Sigma-Aldrich) and histamine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 5 × 10−5 M 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin ...
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bioRxiv - Physiology 2019Quote: ... Citrate synthase activity was measured on a 96-well plate using a commercially available kit (Sigma Aldrich, CS0720).
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bioRxiv - Microbiology 2019Quote: ... supernatants were collected and analyzed for total GLP-1 levels by ELISA (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Genomics 2021Quote: ... U2OS cells in 6-well plates were transduced at 50% confluence with 200 μL of 10x concentrated lentivirus and 5 μg/mL polybrene (EMD Millipore TR-1003-G). Approximately 4 days after transduction ...
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bioRxiv - Neuroscience 2019Quote: ... These animals were raised at 20°C throughout the experiment and transferred to nematode growth medium (NGM) plate supplemented with 250uM 5-fluoro-2′-deoxyuridine (FUDR; Sigma-Aldrich, St. Louis, USA) to prevent progeny production ...
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bioRxiv - Neuroscience 2022Quote: ... each well of a 6-well plate (Axion Biosystems, catalog no. M384-tMEA-6W-5) was coated with 1 mg/mL Poly-L-lysine (Sigma, catalog no. P2636-100MG). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were maintained in culture at 37°C and 5% CO2 in 10cm plates with 10ml of media containing DMEM (#11995-065, Sigma-Aldrich, St. Louis, MO) and 10% FBS (#30-2020 ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were isolated and maintained for 5-12 DIV on 35 mm coverslips (3.5×105 cells/plate) coated with poly-L-lysine/laminin matrix (Sigma-Aldrich, St. Louis, MO, USA). Primary neurons were grown on Neurobasal medium (Gibco BRL ...
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bioRxiv - Developmental Biology 2021Quote: RNA was extracted from whole implantation sites at E6.5 using Trizol (Cat. No. T9424, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... The majority of whole-mount preparations were cleared in a solution of 2,2’thiodiethanol (Sigma-Aldrich), mounted on objective slides and analyzed ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole cell lysates were harvested using ice-cold RIPA buffer with 1x protease inhibitor (Sigma, P8340) and 1x phosphate inhibitor (Sigma ...
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bioRxiv - Pathology 2019Quote: ... Whole blood was mixed with an equal volume of Alsever’s solution (Sigma Aldrich, cat. number A3551). Mice were then sacrificed by cervical dislocation ...
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bioRxiv - Neuroscience 2021Quote: ... E12.5 and E13.5 whole heads were submerged in 4% paraformaldehyde in PBS (PFA; Millipore Sigma, 818715). E15.5 and E17.5 brains were dissected ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... the whole cell extract was applied to 1 mL of anti-Flag M2 affinity gel (Sigma) pre-equilibrated with buffer H ...
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bioRxiv - Cancer Biology 2020Quote: ... and whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Sigma-Aldrich 11836170001). Alternatively ...
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bioRxiv - Cancer Biology 2020Quote: ... The whole cells extract was sonicated and incubated with 60 μl of Ni-NTA agarose (Sigma) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared using SDS-PAGE sample buffer supplemented with benzonase nuclease (Sigma, E1014) and boiled for 5 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting whole cell suspension was successively washed with Gey’s balanced salt solution (GBSS, Sigma-Aldrich). Then the cell pellet was resuspended in GBSS supplemented with 10% fetal calf serum and 10 U/ml DNAse I ...
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bioRxiv - Physiology 2019Quote: ... whole brains were frozen for 1 minute in −35°C 2-methyl-butane (Isopentane, Sigma-Aldrich) and stored at −80°C ...
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bioRxiv - Cancer Biology 2019Quote: ... and whole cell lysates were made in a lysis buffer containing 10% NP-40 (Sigma-Aldrich), 1 mM EDTA ...
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bioRxiv - Genomics 2021Quote: ... whole lichen thalli were collected and stored directly in RNAlater (Sigma-Aldrich Chemie GmbH, Munich, Germany). RNA was isolated from both chemotypes of P ...
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bioRxiv - Biochemistry 2021Quote: ... Both samples were incubated in 1 mL of human serum from clotted whole blood (Millipore-Sigma) for 5 days at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Both samples were incubated in 1 mL of human serum from clotted whole blood (Millipore-Sigma) for 5 days at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by a Ficol gradient (Merck Millipore) and cryopreserved in 90% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: shRNA clones were obtained from the whole RNAi human library for shRNA mediating silencing (Sigma, Aldrich) maintained at IISER ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated retinas were mounted whole over a 1–2 mm2 hole in nitrocellulose filter paper (Millipore) with the photoreceptor layer side down and transferred to the recording chamber of a two-photon microscope for imaging ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were obtained from whole blood EDTA samples and by density centrifugation using histopaque® (Sigma). Samples were selected based on cell viability (at least 80% or higher ...
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bioRxiv - Developmental Biology 2023Quote: Fixed, whole embryos (E8.5, 4-8 somite stages) were immunostained against SOX9 (AB5535, mouse, EMD Millipore) and E-cadherin (610181 ...
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bioRxiv - Pathology 2023Quote: ... whole blood was layered on top of a solution of Histopaque H1117 and H1009 (Sigma Aldrich) and centrifuged for 20 min at 2000 rpm with brake off ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were separated from American crow and robin whole blood using a Histopaque-1077 (Sigma-Aldrich) gradient as previously described (39) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... whole ovaries were treated with a 0.8 mg/ml collagenase solution (Sigma Aldrich, 9001-12-1) at 18°C with agitation for 3.5 hours at 60 RPM ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were separated from the whole blood using a Histopaque-Ficoll gradient (Sigma-Aldrich, Munich, Germany) and washed twice by centrifugation at 1500 rpm for 10 minutes to remove the platelets ...
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bioRxiv - Cell Biology 2023Quote: ... whole-cell extracts (WCE) were prepared by lysing cells in ice-cold RIPA lysis buffer (Millipore). WCE ...
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bioRxiv - Microbiology 2024Quote: ... we filtered 500 mL of whole seawater onto 0.2 µm polyethersulfone filter cartridges (Sterivex-GP, Millipore). After filtering ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Mouse IgG (whole molecule)-ATTO 488 antibody (1:500, Sigma Aldrich 62197-1ML-F, RRID:AB_1137649) diluted in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Whole fly guts and fish larval intestines were mounted on microscope slides using FlouromountTM (Sigma; F4680) and visualized with a spinning-disk confocal microscope (Olympus IX-81 motorised microscope base with Yokagawa CSU 10 spinning-disk scan-head).
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysate was incubated with Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich M8823-1ML) or IgG beads for 3 hours then washed with lysis buffer 3 times ...
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bioRxiv - Immunology 2024Quote: ELISPOT 96 well plates (MultiScreenHTS IP Filter Plate, 0.45 µm, Sigma-Aldrich) were precoated with 2 µg/ml NP-BSA in PBS over night at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Tspan12 siRNA duplexes (#1: 5’-GCUUAUCUUUGCCUUCUCCTT-3’ and 5’-GGAGAAGGCAAAGAUAAGCTT-3’; #2: 5’-AUGAGGGACUACCUAAAUATT-3’ and 5’-UAUUUAGGUAGUCCCUCAUTT-3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... and 48 h later viable cells were sorted on MA900 Multi-Application Cell Sorter (Sony Biotechnology) and cultured in fresh DMEM/IMEM medium (Sigma, UK). Cells were grown for 10 days ...
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bioRxiv - Cell Biology 2020Quote: ... 1×105 cardiomyocytes were seeded in a small drop on the centre of the MEA chip (Multi Channel Systems, 60MEA200/30iR-Ti-gr) coated with 10 μg/mL fibronectin (Sigma, F1141). After 5 h ...
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bioRxiv - Microbiology 2019Quote: ... Cellular responses to Chlamydia infection were analyzed via BD LSRFortessa cell analyzer (Becton Dickinson; Franklin Lakes, NJ) or by Multi-spectral Imaging Flow Cytometer Amnis Image StreamX MKII (EMD Millipore). Data were interpreted by using FlowJo v10 (FlowJo ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue sample was trimmed to 2×3×0.2 mm3 and scanned with a multi-beam serial-section scanning electron microscope (Sigma, Carl Zeiss) in 4×4 nm2 pixels and 33 nm slice thickness ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were counted using a Neubauer Chamber and seeded on coverslips into multi-well culture dishes (Falcon) pre-treated with 0.1% poly-D-lysine (Sigma-Aldrich, #P7280) at a density of 12.500 cells/cm2 in plating medium containing antibiotics (streptomycin/penicillin 100 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and serotonin (5-HT, Sigma-Aldrich, Figure 5) or using blue light on the lumbar spinal cord of ChR2-expressing pups (Vglut2Cre;ChR2floxed ...