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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Cancer Biology 2021Quote: ... The PTBP1 ORF was PCR amplified and subcloned by gibson cloning into pET21A+ (Novagen) digested by XhoI and NheI ...
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bioRxiv - Physiology 2021Quote: ... we cloned each ORF without stop codon into the pIEx-4 expression vector (Novagen) in frame with the vector-encoded carboxy terminal 6 × His-tag and sequenced the resulting constructs ...
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bioRxiv - Immunology 2022Quote: ... ORFs were synthesized as gBlocks (IDT) and assembled using PCR (KOD mastermix, Milipore Sigma) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... polyoxyethylene(4)lauryl ether (Brij L4®), sodium tetraborate decahydrate (≥99.5%) and N,N,N,N-tetramethyl-ethylenediamine (TEMED, 99%) were purchased from Sigma Aldrich, US ...
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bioRxiv - Genomics 2021Quote: ... after which it was quenched with 5 mM of ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA, Sigma, E3889). The quenched reaction was subsequently centrifuged for 5 minutes at 300 rcf and the supernatant was carefully discarded ...
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bioRxiv - Neuroscience 2022Quote: ... For zinc chelation N,N,N,N-Tetrakis (2-pyridylmethyl) ethylenediamine (TPEN) (Cat# P4413-50MG, Sigma-Aldrich, St Louis, MO, USA) solution was freshly prepared in 10%-ethanol (Cat#100983 ...
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bioRxiv - Neuroscience 2022Quote: ... ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA; Cat#E3889, Sigma-Aldrich, St Louis, MO, USA) 1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... fully oxidized and fully reduced samples were generated by the addition of 20 mM N,N,N′,N′-Tetramethylazodicarboxamide (diamide, Sigma Aldrich) or 100 mM Dithiotreitol (DTT ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were washed 1 hour x6 and then transferred to CUBIC1 solution (25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl)ethylenediamine (Sigma 122262), 25 wt% urea and 15 wt% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: Tissue for wholemount imaging was then cut into pieces of 1cmx1cm and cleared while shaking on 37°C in CUBIC-1A (5% N,N,N′,N′-Tetrakis(2-hydroxypropyl) ethylenediamine (122262, Sigma-Aldrich), 10% Urea (U5378 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then washed in 0.2% Triton and placed in CUBIC R1 solution: 25% N,N,N’,N’-tetrakis (Sigma, #122262-1L), 15% Triton X-100 ...
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...
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bioRxiv - Microbiology 2020Quote: ... α-His(Sigma, cat# H1029, 1: 10,000), α-HA (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... anti- HIS from Novagen (70796-3), anti-TGN38 from BD Transduction (610898) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-His (Sigma-Aldrich, H1029, RRID:AB_260015), anti-Strep (IBA GmbH ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HIS (Sigma H1029 1:2,500) or anti-MBP (New England Biolabs E8032 ...
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bioRxiv - Plant Biology 2024Quote: ... or His-beads (Millipore, 70666-4) or MBP-beads (Smart-Lifesciences ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating 40 μm coronal slices were incubated in a monoclonal antibody against parvalbumin (PV) (1:1000, Sigma P3088) for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Microbiology 2023Quote: ... HCoV-OC43 rN was additionally characterized by using mouse anti-OC43 N mAb (Sigma-Aldrich # MAB9013) followed by IRDye® 800CW Goat anti-Mouse IgG Secondary Ab (LI-COR # 926-32210).
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Cancer Biology 2024Quote: ... HA-tag was stained using either rabbit and HA-tag from Sigma-Aldrich (catalogue no. H6908) or mouse anti-HA-Tag (6E2 ...
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bioRxiv - Microbiology 2020Quote: ... 24 hours old bacterial culture was mixed with few drops of 1% of N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) solution (Sigma-Aldrich, USA) on Whatman No ...
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bioRxiv - Cell Biology 2021Quote: 1.5 µM ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) or 5 µM cyclopiazonic acid (CPA, CalBiochem-EMD Millipore). For Ca2+ replenishment ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were quenched by adding 5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) (Sigma-Aldrich, E3889) and pelleted afterwards ...
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bioRxiv - Bioengineering 2023Quote: Cells were incubated in 5 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid EGTA (Sigma-Aldrich, E3889) diluted in Endothelial Growth Medium at 37°C and 5% CO2 for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... and anti-HA (mouse monoclonal clone HA-7 (cat. no. H3663, Sigma Life Science)) ...
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bioRxiv - Immunology 2021Quote: ... or a monoclonal mouse anti-tubulin antibody (Sigma-Aldrich, Clone B-5-1-2). Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:800 mouse anti-acetylated tubulin (clone 6-11B-1, Millipore-Sigma, cat#: MABT868), 1:600 rabbit anti-FMRFamide (Immunostar ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:800 mouse anti-acetylated tubulin (clone 6-11B-1, Millipore-Sigma, cat#: MABT868), 1:600 rabbit anti-FMRFamide (Immunostar ...
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bioRxiv - Genomics 2020Quote: ... mouse anti-acetylated tubulin antibody (clone 6-11B-1, Sigma, St Louis, MO, USA), mouse anti-alpha-tubulin (A11126 ...
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bioRxiv - Biophysics 2020Quote: ... mouse anti-β-actin monoclonal (clone AC-74) (Sigma-Aldrich A2228; 1:20,000 dilution) and rabbit affinity-isolated anti-FLAG polyclonal (Sigma-Aldrich F7425 ...
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bioRxiv - Biochemistry 2020Quote: ... mouse monoclonal anti-HA (MMS-101p; Covance) and anti-FLAG (clone M2; Sigma-Aldrich); Alexa Fluor 488-conjugated (Cat.# ab181448 ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-α-actinin1 clone BM-75.2 (Sigma-Aldrich A5044, IF 1/50).
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-cortactin clone 4F11 (p80/85) (Sigma-Aldrich 05-180, IF 1/50), mouse anti-filamin A clone PM6/317 (Sigma-Aldrich MAB1678 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-acetylated α-tubulin clone 6-11B-1 1:100 (Sigma Aldrich) and mouse polyclonal anti-PAR2 1:100 (T ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-acetylated α-tubulin clone 6-11B-1 1:2000 (Sigma Aldrich). Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-β-actin (1 µg/mL, mouse monoclonal, clone. AC-15; Sigma-Aldrich) antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit polyclonal calbindin antibody (C8666) and mouse monoclonal NeuN antibody (clone A60; Millipore, MAB377B) were from Sigma-Aldrich (Taufkirchen ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-GluR2 (clone 6C4) Alexa Fluor 488 (1:1000; Millipore, Cat#MAB397A4) and mouse monoclonal anti-HA (clone 2-2.2.14 ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies used: anti-γ-H2AX (mouse, 1/200, Millipore, clone JBW301), anti-TH (chicken ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse α-®-actin (Sigma, clone AC-15, #A3854, used at 1:25,000). The HRP conjugated secondary antibodies were Goat α-Mouse (Jackson ImmunoResearch 115-035-003 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti adenomatous polyposis coli antigen (APC; clone CC1; Millipore, OP80-100UG; 1:100). Secondary antibodies were FITC conjugated goat anti-mouse IgG (Invitrogen ...