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Citations for Millipore Sigma :
901 - 950 of 7883 citations for Lassa Fever Virus GP2 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... P12-ICHIKAWA cells were infected with Lenti-FUW-Luc-mCh-puro virus and selected in liquid culture with puromycin (Sigma-Aldrich) 2.5µg/mL for 7 days following mCherry+ cell sorting.
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56 °C ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Synthetic Biology 2021Quote: ... viral transductions were performed overnight in a 6-well dish using 800 µl of standard ESC medium with 200 µl virus-containing supernatant supplemented with 8 µg/ml polybrene (TR-1003; Sigma-Aldrich). The selection of positive clones was performed by supplementing the culture medium with 1µg/ml puromycin for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56°C ...
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bioRxiv - Genomics 2021Quote: The titer of lentivirus was determined by transducing 500,000 cells with varying amount (0, 0.5, 1.0, 2.0, 4.0 and 8.0 µL) of concentrated virus and polybrene (Millipore, TR-1003-G, 8 µg/mL,). Viral transduction was performed by centrifuging the lentivirus and cell combination at 1000 RCF for 90 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... On the following day the virus-containing medium was collected and filtered using 0.45 µm filter (Millipore Sigma, Cat. no: SLHVM33RS). Fresh HEK293T cells were infected by adding the lentivirus-containing medium to culture medium followed by 1 to 2 day incubation ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Microbiology 2022Quote: KSHV BAC16 virus stock was prepared by inducing lytic cycle in iSLK-BAC16 cells with Doxycycline (1 µg/ml; Sigma-Aldrich) and sodium butyrate (1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...
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bioRxiv - Cell Biology 2023Quote: ... cell population potentially harboring latent virus was sorted and stimulated with 10 ng/mL Phorbol 12-Myristate 13-Acetate (PMA, Sigma Aldrich) followed by sorting the GFP (+ ...
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bioRxiv - Biophysics 2022Quote: ... cells at a density of 3×10^6 cells/mL at a ratio of 50 mL virus for 800 mL cells and simultaneously stimulated with 3.75 mM valproic acid (VPA; Sigma Cat# P4543). Pellets were harvested from cells by centrifugation at 48-72 hours after infection and snap frozen.
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bioRxiv - Microbiology 2023Quote: ... Neutralization experiments were performed by diluting the appropriate pseudotyped virus with a 4-fold serial dilution of Albumin (Sigma-Aldrich, A8763), alpha-1-antitrypsin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... Wild-type 3T3 cells at 60-80% confluency were incubated for 24 h with 100X virus suspension and 8 µg/ml of Polybrene Transfection Reagent (Millipore Sigma). To maintain stable knockdown ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich), for 60 minutes while centrifuging at 600 rpm at RT followed by 4 hr incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... the gradient-purified virus was subjected to buffer exchange using the Pur-A-Lyzer™ Maxi Dialysis Kit (20k MWCO) (Sigma) at 4°C overnight to replace the iodixanol with PBS.
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... reporter virus particle-containing supernatant was passed through a 0.22 µm Millex SteriFlip Vacuum-driven Filtration System (Sigma-Aldrich, Burlington, MA) before aliquoting and storage at −80°C ...
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bioRxiv - Plant Biology 2024Quote: ... Twelve-day-old quinoa plants were inoculated on their first pair of true leaves with 20 µl of the virus extracts by mechanical damage using silicon carbide (Sigma-Aldrich). Plants were kept in growth chambers ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Immunology 2024Quote: ... dissociated organoid cells were seeded in virus-containing organoid culture media with 5 μg/ml polybrene (EMD-Millipore TR-1003-G) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Neuroscience 2019Quote: Slides for TPH-OTR immunohistochemistry were blocked in 5% NDS in PBS for 1 hr and then incubated in sheep anti-TPH primary antiserum (T857501VL, Sigma-Aldrich, St. Louis, MO; 1:1200) overnight at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers, MA, USA; sheep anti-αMSH, 1:1000, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS + 1% P/S + 0.1 μM phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) + 1μM Ionomycin (Sigma Alrich ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilization and blocking of samples was performed in blocking solution (10% FCS, 0.3% Saponin (47036, Sigma-Aldrich) in PBS 1x ...
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bioRxiv - Immunology 2019Quote: ... in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA) (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed 8 times and avidin-peroxidase (2.5 μg/mL in PBS / 10 % FCS. Sigma) was added for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... a 15-minute chase was performed with normal medium (0.5% FCS) supplemented with 10 µM thymidine (Sigma) to remove unincorporated EdU and cells were fixed and permeabilized with 3.7% FA and 0.5% Triton X-100 for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in FluroDishes (World Precision) and imaged in DMEM supplemented with 10% FCS (Sigma-Aldrich), 100U/ml penicillin and 100μg/ml puromycin (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... the spinal cord was placed in a plexiglass tube and immersed in fluorinert (FC-40 Sigma Aldrich) to reduce background signal ...
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bioRxiv - Microbiology 2021Quote: ... Procyclic forms (PCF; strain 427) were cultured in modified SDM-79 with 10 % heat-inactivated FCS (Sigma) at 27°C ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... A trapped bead was manually manipulated by a motorized XY stage (BIOS-225T and FC-101G, Sigma), and contact of the bead with the cilium during oscillation for 1.5 h was monitored by ciliary GCaMP6 or mCherry fluorescence imaging or bright-field imaging of the bead ...
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bioRxiv - Cancer Biology 2019Quote: ... one cells have adhered remove culture medium and replace with diluted lentiviral media (500µl lentiviral media, 1.5ml DMEM, 10% FCS, 5% PenStrep, 5-10µg Polybrene (Sigma)) ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and resuspended in RPMI supplemented with 10% FCS and 1% penicillin streptomycin (Sigma Aldrich) (complete media ...
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bioRxiv - Pathology 2020Quote: ... stimulated for 24 hours in 10% FCS DMEM with LPS (1 µg/ml, Salmonella Minnesota; Sigma-Aldrich), BLP (Pam3Cys-Ser-(Lys)4 ...
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bioRxiv - Systems Biology 2019Quote: ... Subsequently, cells were washed two times with a washing solution (5% FCS, 0.05% BSA (Sigma-Aldrich; A2058), 0.02% NaN3(Carl Roth GmbH & Co ...