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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for HLA A*0201 HPV16 E7 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of Cypridina noctiluca luciferase and Omicron RBD or its mutants were co-transfected (9:1) into HEK293 cells using X-tremeGENE 9 (Millipore-Sigma XTG9-RO). After 16 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.5 pmole protein was used for JASPer-FLAG and aromatic cage mutant and for JJ-complex and aromatic cage mutant and pre-coupled to 5 µL FLAG-M2 beads (Sigma-Aldrich) (10 µL 50% slurry ...
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bioRxiv - Biochemistry 2019Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... which were then plated in black 96-well plates in the presence of 5 mM complex I substrates glutamate and malate with or without 5 µM CCCP (carbonyl cyanide m-chlorophenyl hydrazone, Sigma-Aldrich) and 1 µM TMRM (tetramethylrhodamine ...
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bioRxiv - Microbiology 2021Quote: We resuspended wild-type and mutant yeast cells in 50 mM potassium phosphate buffer to a final concentration 5 × 107 cells/ml and supplemented the suspension with filipin (filipin complex from Streptomyces filipinensis; Sigma F9765) to a final concentration of 5 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... High Five™ insect cells for the expression of PP2A A/C complex were cultured in EX-CELL 405 Serum-Free medium (Millipore-Sigma) at 27°C with 130 rpm ...
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bioRxiv - Immunology 2020Quote: ... Agarose was pelleted and placed at rotation for five minutes with Low Salt Immune Complex Wash Buffer (Millipore, Cat #20-154), then pelleted ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... the fluorescent-DNA labelled antibody complex was purified using 100 kDa molecular weight cut-off Amicon ultra filtration columns (Millipore #UFC510096) and stored in PBS buffer containing 0.5M NaCl at 4°C for use in SeqStain assays.
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bioRxiv - Molecular Biology 2020Quote: ... Samples of PARP-1 CAT domain in complex with PARP inhibitors for NMR were made by adding inhibitors (50mM in [2H6] DMSO, Sigma-Aldrich) to a final ratio of 1:1.5 (veliparib ...
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bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Developmental Biology 2020Quote: ... Four to six larval body wall tissues containing muscle-epidermis complex were homogenized in 21 μl of RIPA buffer (Sigma, #R0278) supplemented with a cocktail of protease inhibitors (Complete mini ...
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bioRxiv - Cell Biology 2021Quote: ... Replenishment of cholesterol was performed by incubating the cholesterol-depleted cells in 10 mM MβCD-cholesterol complex (1:1 mol/mol; Sigma Aldrich: C3045) at 37°C for 30 min38 ...
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bioRxiv - Cell Biology 2022Quote: Drugs used in this study include the Arp2/3 complex inhibitor CK-666 (48) and the formin agonist IMM-01 (49) and were purchased from Sigma-Aldrich. For drug treatments prior to immunoprecipitation experiments ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... TAP- and HTP-tagged proteins were detected after a unique incubation of the membranes with Peroxidase Anti-Peroxidase Complex produced in rabbit (1:2,000; Sigma, Cat# P1291). Chemiluminescence signals were revealed on an Amersham™ Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed by dounce homogenization and complex formation was initiated with the addition of 25 mU/mL Apyrase (Sigma-Aldrich) and 10 μM ET-1/ IRL1620 (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
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bioRxiv - Cell Biology 2023Quote: ... Then 0.4 µL of the secondary-readout (S-RO) complex was diluted in 200 µL EC buffer [10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911) ...
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bioRxiv - Neuroscience 2024Quote: Oligomycin and antimycin A (inhibitor of mitochondrial complex V and III respectively) were purchased from Sigma-Aldrich (O4876 and A8674 respectively).
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bioRxiv - Cell Biology 2024Quote: ... a complex of siRNA and DharmaFECT 2 was prepared in serum-free DMEM (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) at a volume of 100 μL/well according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... human insulin was purchased by SIGMA and was used at a final concentration of 7ug/ml ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with human albumin (Sigma, #A0237), sodium DL-lactate (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human serum albumin (Sigma, Catalog # A9511) and human pituitary FSH (National Hormone and Pituitary Program ...