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Citations for Millipore Sigma :
901 - 950 of 6476 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The precleared lysate was incubated with 30µl FLAG beads (anti-FLAG M2 Affinity Gel, Millipore Sigma) overnight ...
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bioRxiv - Biophysics 2022Quote: ... Wnt display was confirmed by mouse Monoclonal ANTI-FLAG M2-FITC antibody (FLAG-FITC, Sigma F4049), showing comparable display between the full-length and NC-linker truncated versions.
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bioRxiv - Plant Biology 2019Quote: ... proteins in fusion with 2xStrep-FLAG were purified with Anti-FLAG M2 Magnetic Beads (Sigma Co.). Purification was carried out according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cell Biology 2020Quote: ... MFG-E8-eGFP was then purified by FLAG affinity chromatography using M2-FLAG agarose beads (Sigma). Bound protein was eluted using an excess of FLAG peptide (Genscript ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-FLAG M2 affinity gel (A2220) and 3 x FLAG peptide (F3290) were from Sigma-Aldrich, protein A or G beads were from Invitrogen (10004D) ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation against flag was performed using EZview™ Red ANTI-FLAG® M2 Affinity Gel (Sigma) overnight at 4°C followed by washing in IP buffer ...
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bioRxiv - Cell Biology 2021Quote: The flag-tag immunoprecipitations were carried out using FLAG-M2 coated agarose beads (Sigma-Aldrich #F2426) for 2 hours at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged Srb10/ Cdk8 was recovered by immunoprecipitation with anti-FLAG-conjugated M2 agarose beads (Sigma). Samples were washed two times in KLB followed by two times in kinase assay buffer (KAB ...
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bioRxiv - Plant Biology 2022Quote: ... Captured proteins were eluted off FLAG beads using 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich). Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of FLAG/HA-AGO2 was performed with Anti-FLAG M2 Magnetic Beads (Sigma Cat # M8823). Beads were washed twice with TBS buffer (50 mM Tris HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-FLAG M2 antibody staining was at 1:1000 (Monoclonal ANTI-FLAG M2 antibody, Sigma F1804) for overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-Tel1 immunoprecipitation was performed with 2µl of anti-FLAG antibody (M2 monoclonal, Sigma, Ref F1804) and incubation at 4°C for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-FLAG (Sigma, F3165). Quantitative PCR was performed using SYBR Green (Bio-Rad ...
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Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiencybioRxiv - Plant Biology 2020Quote: ... anti-FLAG antibody (Sigma-Aldrich, SAB4301135); anti-GFP antibody(Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Flag (Sigma, F1804, 1:1000) and anti-HA (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg of Flag (Sigma #F1804) antibody were added to each CTRL/TRIM67 IP samples ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG (Sigma Aldrich, F1804, 1:10,000), HA-HRP (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Flag antibody (Sigma, F3165)
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bioRxiv - Molecular Biology 2020Quote: ... ANTI-FLAG® M2 (Sigma, F1804), HA (Biolegend #901515) ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-Flag (AB_259529, Sigma-Aldrich). After five washes ...
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bioRxiv - Cell Biology 2020Quote: ... ANTI-FLAG M2 Magnetic Beads (SIGMA) were prepared as described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... and mouse Flag M2 (Sigma-Aldrich). Rabbit Fbxo45 antibody was kindly provided by Kojo S ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-mouse monoclonal antibody (Sigma-Aldrich) was used at 1:5000 for western blotting and at 1:500 for immunofluorescence ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG tag from Sigma Aldrich (F3165); SHIP2 and phosphorylated SHIP2-Y1135 from Cell Signaling Technologies (#2839 and 5445 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse m2 anti-FLAG (Sigma #F1804), rabbit anti-Tgβ-tubulin (1:10,000 dilution) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Flag antibody (Sigma #F3165) 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Flag (F1804; Sigma-Aldrich), rabbit anti-macroH2A1 (Ab37264 ...
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bioRxiv - Cell Biology 2020Quote: ... and FLAG (M2 mouse mAb, Sigma) were incubated overnight at 4ºC ...
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bioRxiv - Cancer Biology 2020Quote: ... 1:5 000 FLAG (Sigma, F1804), 1:10 000 AR (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-FLAG (1:4000) (Sigma), rabbit anti-P66 (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-Flag HRP-conjugated (Sigma, A8592), anti-p-SEK1/MKK4 (Cell Signaling ...
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bioRxiv - Microbiology 2019Quote: ... anti-FLAG (Sigma F1804, 1:1000), anti-MNDA (Cell Signaling Technology 3329 ...
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bioRxiv - Molecular Biology 2021Quote: Antibodies against FLAG (F1804, Sigma Aldrich), IPMK (custom rabbit polyclonal antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-Flag (1:200, Sigma), mouse anti-V5 (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-FLAG antibody (Sigma F-1804) was used to detect the 3xFLAG tagged HOXB1 protein in the eluted samples.
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bioRxiv - Biochemistry 2022Quote: ... primary (Sigma anti-Flag M2 antibody) and secondary antibody (Invitrogen anti-mouse antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-FLAG mAb (Sigma, USA), mouse anti-GFP mouse mAb (Affinity Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies against FLAG (Sigma-Aldrich, #F7425), Rab7a (# NBP1-87174 ...