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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 43] using rabbit polyclonal antibody Synapsin-1 (SYN-1, 1:200; Millipore), goat anti-guinea pig antibody VGlu2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: ... solutions of 89Zr-labeled TDM1 (4 μCi/μg) were prepared in PBS (pH 7.5) containing 1% w/v human serum albumin (HSA, Sigma) and 0.1% w/v sodium azide (NaN3 ...
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bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibody staining was carried out overnight at 4°C in PBS + 0.1% Triton X-100 and 1% BSA (Sigma). Primary antibodies used were anti-pERK1/2 1:200 (4370S ...
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were then incubated overnight at 4°C with the following primary antibodies rabbit AB5804 GFAP (1:2000, Millipore) and mouse N0142 NF200 (1:3000 ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were incubated overnight at 4°C with primary antibodies against MAP2 (1:1000, #M9942, Sigma-Aldrich, MO, USA), Iba1 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... and the resultant supernatants were incubated for 1 h at 4°C with anti-Flag M2 beads (Sigma-Aldrich). The beads were washed three times ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4 °C with a monoclonal anti-RBPMS antibody (1:500, Rabbit, ABN1362, Merck Millipore) for the retina ...
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bioRxiv - Molecular Biology 2021Quote: ... the remaining pre-cleared supernatant was incubated rotating at 4 °C overnight with 1 µL of FLAG antibody (Sigma). The immune complexes were captured for 2 hr at 4 °C with 50 μL of pre-blocked Protein-A Dynabeads ...
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bioRxiv - Cell Biology 2022Quote: ... BJ-5ta cell lines were cultured in DMEM supplemented with a 4:1 ratio of Medium 199 (Sigma-Aldrich) and 0.01 mg/ml hygromycin B (Invitrogen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, REF-D9542, Sigma, USA) for 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... the conjugate underwent buffer exchange three times into 1× DPBS pH 7.4 using a 4-ml Amicon Ultra centrifugal filter (30 kDa MWCO, Millipore). The conjugate was characterized using SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma;F3777) in blocking buffer containing 1 to 5% bovine serum albumin in 0.25–0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 g for 1 hr at 4° C and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). GFP nanobody was coupled to CNBr-activated Sepharose Fast Flow beads (GE healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 x g at 4° C for 1 hr and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). 2 mL GFP nanobody resin was incubated with the sample with agitation at 4 °C for 1 hr ...
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bioRxiv - Neuroscience 2020Quote: ... plus 0.1% Tween-20 and incubated overnight at 4 °C with primary antibodies for: anti-actin (1:5000, Sigma) anti-PCDH19 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Cell Biology 2021Quote: ... and incubation overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898, 1:1,000; rabbit anti-H3K27me3, Millipore, 07-449 ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898, 1:3,000; rabbit anti-H3K27me3, Millipore, 07-449 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed for 15min in 4% PFA and stained with α-alpha-actinin antibody (A7811, Sigma, 1:1000).
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bioRxiv - Biochemistry 2021Quote: ... by the crush and soak method68 and concentrated to a volume of 0.5 – 1 mL using centrifugal filters (Amicon Ultra-4 /-15, Millipore) with a molecular weight cutoff (MWCO ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in an antibody incubation solution (in PBS: 1% bovine serum albumin (Sigma), 1% normal goat serum (Vector) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma-Aldrich) in 50 mM Na phosphate ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were carried out in 20 mM Tris buffer (pH 7.5) supplemented with 4 mg ml-1 CMC sodium salt (Sigma), 8 mg ml-1 microcrystalline cellulose (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 200x molar excess of succinimidyl 4-[N-maleimidomethyl]cyclo-hexane-1-carboxylate (SMCC-Sigma-Aldrich #M5525) for 2.5 hours at room temperature to generate maleimide-DNA capable of sulfhydryl-reactive crosslinker chemistry with peptides with terminal cysteines ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated overnight at 4°C with anti-pan-ADPribose reagent at 1/400 dilution (Millipore-Sigma # MABE1016). ADPribose was detected using anti-rabbit IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A32740) ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing solubilized IBs was diluted 1:4 in lysis buffer containing 8M urea and filtered through a 0.22 μm membrane (Millipore). Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Cell Biology 2020Quote: ... 3D aggregates were fixed using 4% PFA for 1 hour followed by permeabilization in 0.1% Triton-X 100 and 0.2% BSA (Sigma-Aldrich). Samples were then stained with primary antibody for PAX6 (clone ...
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bioRxiv - Developmental Biology 2022Quote: ... tissue sections were probed overnight at 4°C with primary antibodies including rat α-Endomucin (1:250; Millipore; MAB2624); mouse α-Cardiac Troponin T (1:250 ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBST overnight at 4°C with agitation or with 1:5000 beta actin mouse monoclonal antibody (Sigma A5441) in 2% milk powder in PBST for 2 hours at room temperature with agitation ...
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bioRxiv - Cell Biology 2022Quote: ... coverslips were carefully submerged into a 24-well plate with 1 ml of 4% Formaldehyde (FA, 36.5–38%, Sigma) and 4% acrylamide (AA ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with anti-PC antibody (1:500, HPA043922, Millipore-Sigma, Darmstadt, Germany), anti-beta tubulin antibody (1:2000 ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with anti-PC antibody (1:500, HPA043922, Millipore-Sigma, Darmstadt, Germany), anti-beta tubulin antibody (1:2000 ...
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Neuroscience 2021Quote: During patch-clamp recordings, bath application of TTX (1 µM, Alomone, Israel) or 4-AP (0.3 - 600 µM, Sigma) was done using 0.5 mm diameter quartz tubing positioned ...
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bioRxiv - Microbiology 2021Quote: ... Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore). The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... incubated overnight at 4 °C in primary antibody: rat anti-Laminin-α2 (1:250, Cat. No L0663, Sigma-Aldrich), rabbit anti-Collagen Type I (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Neuroscience 2020Quote: ... Tau aggregates were produced using 1mL tau P301S at 8mg/mL was incubated with 4 mg.mL−1 heparin (Sigma) in PBS plus 30 mM 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting suspension was centrifuged at 25,000 rpm for 1 h at 4 °C using high-speed centrifuge (Sigma) (Wilson et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 20% glycerol) and blocked for 1 hour 4 °C rotating in Buffer C100 with 0.1mg/ml insulin (Sigma, I9278), 0.2mg/ml chicken egg albumin (Sigma A5503) ...
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bioRxiv - Physiology 2022Quote: ... hatching was induced by placing embryos in chilled (4°C) 1 g/L humic acid solution (Sigma-Aldrich, 53680) and incubating them in that solution overnight at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Epidemiology 2020Quote: ... An in-house polyclonal anti-flavivirus antibody (anti-DENV 1-4) and an anti-mouse IgG-FITC antibody (Sigma) were used ...
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bioRxiv - Cell Biology 2021Quote: ... after which the cells were fixed in situ by addition of 1 mL of fixative containing 4% paraformaldehyde (Sigma) and 0.05% glutaraldehyde (25% solution in dH2O ...
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bioRxiv - Zoology 2020Quote: ... We used standard dilutions of hen egg white lysozyme (0.5, 0.8, 1, 2, 4, 8, 10, 20, and 40 µg/ml; Sigma-Aldrich) to prepare a standard curve in each plate ...
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bioRxiv - Microbiology 2020Quote: ... The polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with appropriate antibodies: anti-Arp (dilution 1:1,000; Sigma); anti-FHOD1 (dilution 1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified by centrifugation at 10000 g at 4°C for 1 hour and filtered (0.45 µm pore size, Millipore). Clarified lysates were loaded on Ni-NTA resin columns (Thermo Scientific ...