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Citations for Millipore Sigma :
9351 - 9400 of 10000+ citations for Recombinant Human PDCD5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... ShOpa1 transduced cells were lysed and 35 μg of protein was electroblotted to PVDF membranes (EMD Millipore, Burlington, MA, USA). The membranes were blocked and incubated overnight at 4°C with antibodies against anti-BNIP3 (3769) ...
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bioRxiv - Cell Biology 2024Quote: ... Extracted proteins were resolved on a 10% SDS-PAGE gel and electroblotted onto a PVDF membrane (Sigma Aldrich, Cat # IPVH00005). The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 600-800μg of protein were diluted in 200μl and added to 50μl of anti-FLAG® M2 affinity gel slurry (Millipore, A2220). Samples were incubated for 1 hour rotating at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were loaded onto SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... For shRNA-mediated knockdown, all TRC pLKO.1 vectors (targeting the YTH domain-containing proteins, THOC1, and THOC2) were purchased from Sigma. The TRIPZ doxycycline (Dox)-inducible lentiviral YTHDC1 shRNA vector is purchased from Dharmacon.
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bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The purity of the proteins was examined by SDS-PAGE and the concentration was measured with Bradford reagent (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with primary antibodies against protein of interest (NPR, Santa Cruz, sc-39008, 1:100; GFP, Aveslab, GFP-1020, 1:5,000; FLAG, Sigma, F1804 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein was quantified according to the Coomassie blue method using bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) as a standard (Bradford 1976) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein extracts (50 µg) were then in-gel digested with trypsin overnight (1:100, w/w, Sigma-Aldrich, USA). Peptides were applied to a reverse-phase liquid chromatographer [Acquity UPLC M-Class System (Waters Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... The protein sample after elution was collected and concentrated to 2 mg/mL using a 50kDa molecular weight cutoff concentrator (Millipore).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of protein samples were loaded onto SDS–PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration of lysates was determined and 70 µg protein was loaded onto a 10% SDS-PAGE and subsequently transferred to a PVDF-FL membrane (Millipore). Membranes were incubated overnight at 4°C in primary antibodies (Flag ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC Chromatography system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 25-30µg protein was loaded on resolving 12% SDS-PAGE and later transferred to a methanol-activated PVDF membrane (Millipore-# IPVH00010) by using a semi-dry Trans blot apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by electrophoresis on precast gradient SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore) using a wet transfer tank (Hoefer Instruments) ...
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bioRxiv - Immunology 2024Quote: ... Each 1 ml collected fraction was analyzed for protein concentration using a Nanodrop spectrophotometer (absorbance at 280 nm) and pooled three fractions were further analyzed for protein concentration using Bradford assay (Sigma) and used to measure biological activity to induce Arg1 expression in RAW264.7 macrophage cell line ...
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bioRxiv - Immunology 2024Quote: Activated TKO CD4+ T cells were activated with Cell stimulation cocktail plus protein transport inhibitors (eBioscience) and PMA (500 ng/ml, Sigma) for 4 hrs and then incubated with antibodies targeting cell surface markers ...
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bioRxiv - Immunology 2024Quote: ... Cytoplasmic protein extracts were obtained by incubation of cells with lysis buffer [50 mM NaH2PO4 (Sigma-Aldrich, Cat. No. S8282), 200 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: Gene fragments encompassing the employed proteins and their variants were amplified by polymerase chain reaction and inserted into a pET24d vector (Novagen) via NcoI/XhoI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary emerin (Protein-tech, cat# 10351-1-AP, 1:3,000 dilution) and γ-tubulin (Sigma, cat# T6557, 1:25,000 dilution) antibodies were incubated overnight with rocking at 4°C and secondary antibodies (goat anti-rabbit [cat# 31462 ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant proteins were resolved on a 10% SDS-PAGE and transferred to a nitrocellulose membrane (0.45 μm, Millipore, USA). Membranes were blocked in blocking buffer (5% nonfat milk ...
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bioRxiv - Immunology 2024Quote: WB analysis of protein levels was performed on whole-cell lysates prepared by lysis in radioimmunoprecipitation assay buffer (Sigma-Aldrich) supplemented with Benzonase (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: ... Samples were then further purified to remove protein by filtration at 14,000 rpm for 30 minutes through Amicon® Ultra-0.5 spin columns (Sigma Aldrich) which have a 30 kDa molecular weight cutoff62 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was transferred to a PVDF membrane 0.2 μm (Amersham) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, MILLIPORE). Membranes were blocked with 5% nonfat dry milk in PBS with 0.05% Tween20 (PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein lysates were electrophoretically separated on sodium dodecyl sulfate-polyacrylamide gels and electrically transferred to polyvinylidene fluoride membranes (IPVH00010, Millipore). The membranes were blocked in 5% skimmed milk for 1 hour and incubated with the primary antibodies (Table S6 ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of cell culture was harvested by centrifugation and lysed by incubation for 20 min with 200 µl in BugBuster protein extraction reagent supplemented with 1uL of Benzonase nuclease (Millipore), 0,6 µl of lysozyme (10 mg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EDTA overnight at 4 °C Protein prep was then concentrated with Amicon Ultra-15 Centrifugal Filter Units 3000K (Millipore) to a final concentration of 1.6 mg/ml measured by Bradford assay (Thermo).
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bioRxiv - Neuroscience 2023Quote: Total protein from VM cell populations were harvested using 100 µl of RIPA buffer (R0278, Sigma-Aldrich®, Dublin, Ireland) supplemented with 1% of Protease inhibitor (Roche® ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Immunology 2022Quote: ... growth factors) in mouse whole lung protein were quantified using EMD Millipore’s MILLIPLEX Magnetic Bead Panel (Millipore Sigma #MCYTOMAG-70K), including ...
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bioRxiv - Microbiology 2023Quote: Eluted proteins were concentrated and desalted with an Amicon® Ultra-4 Centrifugal Filter Device (30 kDa MWCO, Millipore Sigma), with equilibration in protein binding buffer lacking imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...