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Citations for Millipore Sigma :
9251 - 9300 of 10000+ citations for Mouse Spermidine Synthase SRM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... followed by incubation in LI-COR blocking buffer containing rabbit polyclonal anti-P2X2 (#APR-003, Alomone labs; 1:2000) and mouse anti-Na+/K+-ATPase (05-369; EMD Merck Millipore) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were the resolved in a 12% SDS-PAGE gel and transferred on a 0.2 µm nitrocellulose membrane and then immunobloted using mouse FlagM2 antibody (Sigma-Aldrich) diluted at 1/500.
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies were goat anti-rabbit and anti-mouse Alexa 488 and Alexa 555 conjugated fluorescence at 1:500 (Millipore). The spinal cord sections were directly coverslipped with Dapi mounting media (Vector ...
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bioRxiv - Biophysics 2022Quote: ... Phagosomes in each chamber were incubated with one of the following mouse monoclonal antibodies against kinesin-1 (MAB1614, EMD Millipore), kinesin-2 (ab24626 ...
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bioRxiv - Cell Biology 2022Quote: ... 2013), rabbit anti-Cep97 (Dobbelaere et al., 2020), rabbit anti-Cnn (Dobbelaere et al., 2008) and mouse anti-Actin (Sigma); all primary antibodies were used at 1:500 dilution in blocking solution (Aydogan et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Smith’s laboratory) mouse ESC lines were routinely cultured without feeders on gelatin-coated plates (0.2% gelatin, Sigma-Aldrich, cat. G1890). Media were changed every 2 days and cells were passaged when approaching confluency (every 2-3 days) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Cell Biology 2022Quote: ... each strip was incubated with 99 μL PBSBT and 1 μL mouse anti-CNB antibody (Sigma-Aldrich, C0581, 1:100), on the nutator for five hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA knockdown vectors for mouse Mdm2 (TRNC0000302276) or human MDM2 (TRCN0000355726, TRCN0000355727, TRCN0000355728, TRCN0000003378 and TRCN0000003380) were from Sigma Aldrich. The pBabe-puro expression vector for CA-AMPK has been described53.
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bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.1 % Triton X-100 for 60 min at room temperature and incubated with mouse monoclonal anti-TUBB3 (Sigma; #T8660) (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: Live TTX-treated WT and Fmr1R138Q hippocampal neurons (14-15 DIV) were incubated with a mouse monoclonal anti-N-terminal GluA1 antibody (1:100; Merk-Millipore) for 10 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Immunology 2020Quote: ... fixed antibodies were detected with horseradish peroxidase-conjugated goat anti-mouse IgA (100 µL / well - 1.5 µg/mL; Sigma-Aldrich) for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Slides were placed in a light-protected humidity-controlled tray and incubated in primary antibody (mouse-anti-Neurofilament 200; clone N52; MAB5266; Sigma) at 1:500 in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were stained with rabbit anti-Actin or rabbit anti-TnI (1:1000; a gift from A. Ferrus) or mouse anti-Tubulin (1:1000, Sigma) and washed with TBS-Triton X (0.1%) ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then incubated for 1 h with secondary antibody a-mouse HRP conjugate 1:10000 (Cell Signalling) and chemiluminescent substrate ultra (Millipore) was applied for 5 min before camera detection in Sapphire Biomolecular Imager (Azure Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-RELN G10 (MAB5364, Millipore 1:2000) or 142 (for human RELN ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were isolated from a single mouse hippocampus in 2 ml of pre-chilled EZ PREP lysis buffer (NUC-101, Sigma) using a glass Dounce tissue grinder (D8938 ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2021Quote: ... then the Duolink In Situ PLA Probe Anti-Rabbit PLUS and Duolink In Situ PLA Probe Anti-Mouse MINUS PLA probes (Sigma) diluted in Duolink Antibody Diluent were applied ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with OXT and AVP primary antibodies (mouse anti-oxytoxin, Gainer Lab, NIH, PS3845,60 and rabbit anti-vasopressin, Millipore PC234L) diluted in 0.1 % saponin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked with 5% bovine serum albumin (BSA) and then probed with the following primary antibodies - mouse anti-GluN2A (1:1000, Millipore), rabbit anti-GluN2B (1:1000 ...
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bioRxiv - Microbiology 2021Quote: Western blot analyses of 3xFLAG-tagged proteins or IgaA were carried out as described previously (Pontel and Soncini, 2009; Pérez Audero et al., 2010) with mouse anti-FLAG monoclonal (Sigma-Aldrich) or rabbit polyclonal anti-IgaA antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The slices then incubated with primary antibodies with denoted dilutions overnight at 4°C (anti-Iba1 goat polyclonal, Abcam ab5076, 1:200; anti-HNA mouse monoclonal, Millipore MAB1281 ...
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bioRxiv - Neuroscience 2022Quote: All samples were immunostained for fluorescence activated cell sorting (FACS) with mouse anti-NeuN conjugated to PE (EMD Millipore, FCMAB317PE) at a dilution of 1:500 with incubation for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then pre-cleared using 25 μL of Protein A agarose beads (Pierce) and then incubated with 25 μL of mouse anti-V5 agarose beads (Sigma) and washed with RIPA buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... R26-CreERT2/R26-Confetti mice received intraperitoneal injections of tamoxifen (TAM; 4mg/25 grams mouse weight, Sigma, St. Louis, MO) dissolved in 10% ethanol/corn oil (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Neuroscience 2019Quote: ... and or Alexa Flour 488 anti-mouse IgG (1:800, A11001, Life) at room temperature for 1h and mounted with DAPI (F6057, Sigma). The sections were observed using fluorescence microscope (Olympus BX35 ...
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... containing 4% PFA (EM Sciences) and 0.1% Triton X-100 and stained with a mouse antibody to α-tubulin (DM1α; SIGMA) and a secondary donkey DyLight 594-conjugated F(ab’)2 mouse IgGs (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... The serum-containing mESCs medium was composed of high glucose DMEM (HyClone) supplemented with 1000 U/mL recombinant mouse leukaemia inhibitory factor (LIF) (Millipore), 100 μM non-essential amino acids (NEAA ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS before their incubation with an anti-HA mouse monoclonal antibody (Sigma) for 2 hours at room temperature ...