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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for UDP β L Arabinofuranose since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2019Quote: ... Ten sexually mature individuals were chosen from each stock to spawn by flowing water stimulation for two hours followed by injection of 0.1 mL of 0.05 mmol/L serotonin (5-hydroxytryptamine, Sigma, USA) into the adductor muscle (Cruz ...
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bioRxiv - Genetics 2019Quote: ... The differentiating cells were split 1:2 with Accutase and seeded with ROCK inhibitor onto Poly-L-ornithine (Sigma), fibronectin (2ug/ml ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... confluent cells were removed from flasks using Trypsin-EDTA (0.05/0.02%) (Biochrom, Germany) and then transferred onto poly-L-lysine–coated (0.01%) (Sigma-Aldrich, USA) glass coverslips (5000 cells/cm2 ...
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bioRxiv - Bioengineering 2019Quote: ... Synthetic Complete (SC) medium was prepared using 6.7 g/L Yeast Nitrogen Base without amino acids from Sigma-Aldrich, 1.92 g/L Synthetic Drop-out supplement without histidine from Sigma-Aldrich and 76 mg/L histidine ...
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bioRxiv - Microbiology 2019Quote: ... Bacteria-free supernatants were incubated at room temperature for 10 minutes with 20 mmol/l of dithiothreitol (Sigma-Aldrich). Supernatant was aliquoted into two tubes ...
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bioRxiv - Microbiology 2019Quote: One liter from each 60-L seawater sample was filtered through a 0.22-μm pore-size Durapore PVDF 47-mm filter (Millipore) in a sterile Sterivex filter unit (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended in complete Neurobasal media and plated at 0.5 × 106 cells/well on 6-well plates coated with 0.1 mg/ml poly-L-lysine (PLL; Sigma Aldrich). Neurons were cultured at 37°C in a humidified incubator with 5% CO2 and medium replaced every 5 days with fresh medium containing B27.
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bioRxiv - Synthetic Biology 2020Quote: ... Antifungal marker characterization experiments were conducted in yeast extract-peptone-dextrose medium (50 g/L YPD broth (Sigma-Aldrich)) supplemented with various concentrations (50 µg/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... both cell lines were cultured in RPMI-1640 medium with L-glutamine and sodium bicarbonate (R8758, Sigma-Aldrich, Germany), supplemented with 10% fetal bovine serum (F7524 ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR experiments were conducted with Top Banana cultures cleared of Wolbachia by rearing for two generations on indicated diet with 0.25 g/L tetracycline (Sigma), followed by >10 generations on regular diet ...
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bioRxiv - Cell Biology 2019Quote: ... Then 2,5 Mл culture medium (α-MEM, 10% FBS, 100 μM L-ascorbic acid 2-phosphate (Sigma-Aldrich, USA), 2 mM L-glutamine ...
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bioRxiv - Biophysics 2019Quote: Clean glass coverslips were placed flat on a clean slide and a 100 μL droplet of 0.01% poly-L-lysine (P4832, Sigma) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... The 40 µL RNA sample destined for CMC-labeling was added to 400 µL BEU+CMC buffer (50 mM Bicine, pH 8.5; 4 mM EDTA; 7 M urea; 200 mM CMC (Sigma)) ...
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bioRxiv - Molecular Biology 2019Quote: ... The sections were mounted onto microscope glass slides coated with a 0.01% solution of (poly)L-lysine (Sigma-Aldrich) or adhered to parafilm ...
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bioRxiv - Genetics 2020Quote: ... CAN1 forward mutation rates were determined by plating cells on SCD-Arg medium supplemented with 60μg/mL L-canavanine sulfate (SCD-Arg+Can; Sigma). For complementation studies in Figure 2B ...
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bioRxiv - Cancer Biology 2020Quote: MCF-7 [28] and MDA-MB-231 [29] cells were cultured in high glucose (4.5 g L−1) Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich) supplemented with 10 % Foetal Calf Serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... The media were supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamine (all from Sigma-Aldrich). Cells were cultured at 37 °C and 5% CO2 and regularly tested for mycoplasma contamination.
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bioRxiv - Neuroscience 2020Quote: ... The tissue was transferred to sterile conditions and enzymatically digested in dissociation medium supplied with 0.16 mg/ml L-cysteine and 70U papain (Sigma) set to pH 7.35 ...
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bioRxiv - Neuroscience 2020Quote: ... and iPSC derived H9N NSCs homozygously carrying the LRRK2 G2019S (GGC>AGC) mutation (ax0310) (Axol Bioscience, Cambridge, United Kingdom) were cultured and expanded on 0.01 % poly-L-ornithine (PLO) (Sigma) and L-15 laminin (L15 medium containing 1:60 natural mouse laminin and 1:41 sodium bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654; 0.21% L-methionine, Sigma #M9625 ...
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bioRxiv - Neuroscience 2020Quote: ... Enucleated eyes were placed in petri dish filled with carboxygenated (95% O2/ 5% CO2) Ames’ media (supplemented with 1.9g/L sodium bicarbonate, pH 7.4, Sigma Aldrich) and retinas dissected ...
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bioRxiv - Cell Biology 2021Quote: ... A 1-μl aliquot of each sample was put on a poly-L-lysine coated glass slide (Sigma, P0425) and observed by confocal microscopy.
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bioRxiv - Bioengineering 2021Quote: ... the cell culture channels of the microfluidic devices were coated with 0.01% (w/v in H2O) poly-L-lysine (PLL) solution (Sigma).
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bioRxiv - Bioengineering 2021Quote: RBCs samples were bonded to Nº 1.5 glass coverslips treated with 100 ng/ml Poly-L-Lysine (Sigma-Aldrich) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Detergents in the samples were exchanged with 100 mmol/L Tris-HCl (pH 8.5) using Microcon filters (10 kDa cut off, Millipore). Tryptic digestion with trypsin (Sequencing Grade Modified ...
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bioRxiv - Biochemistry 2021Quote: Frozen helminths were ground with a pestle in a mortar with liquid nitrogen to a powder with the addition of 0.1 mol/L Tris-HCl (pH 7.6) with 1% protease inhibitor cocktail (Amresko) and 1% phenylmethanesulfonyl fluoride (Sigma-Aldrich). After thawing the extraction buffer protein was precipitated with 100% trichloroacetic acid (final concentration 10%)(Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... and the animals mildly anesthetized with a dose of tricaine methane sulfonate (MS-222; 80 mg/L; Sigma-Aldrich) to facilitate their placement in the tube and minimize their stress ...
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bioRxiv - Neuroscience 2020Quote: ... The dissociated cells were plated at a density of 20000-30000 cells/cm2 on poly-L-lysine (P2636, Sigma) (0.2mg/ml in borate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The surfaces of glass coverslips and plates were pre-coated with 1 μg/μl poly-L-lysine (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then plated on glass coverslips (WVR international) previously coated with PLL (Poly-L-lysine hydrobromide, mol wt 70.000-150.000, Sigma Aldrich) diluted to 1mg/ml in borate buffer (0.1M boric acid ...
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bioRxiv - Cell Biology 2021Quote: ... then triturated and plated on poly-L-lysine coated 8-well glass chambers (Sigma, P2636, Cellvis, C8-1-N) at a density of 25,000 cells/well in 5 % horse serum (Gibco 26050-088 ...
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bioRxiv - Cell Biology 2021Quote: ... murine CD4+ T cells were isolated and activated in a 12 well plate with culture media containing 2g/L D-Glucose-1,2-13C2 (Sigma) for 24 hours ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... cleaned for 30 min with 1 M KOH (Fluka, Bucharest, Romania) and coated for 30 min with 0.01 % poly-L-Lysine beforehand (P4707, Sigma). Samples were rinsed 5 times with deionized water and dried under nitrogen flow ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was triturated to dissociate neurons and plated onto poly-L-lysine (30,000-70,000; Sigma-Aldrich, St. Louis, MO) coated culture dishes with Gibco neurobasal-A culture medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Separation was performed using a reversed phase column (25 cm L x 10 mm D, 5 µm, Sigma-Aldrich) controlled by the LabSolutions software ...
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bioRxiv - Microbiology 2020Quote: ... with and without the addition of tryptophan (100 μL of the 100 mg L−1 stock solution in distilled water and filtered through 0.22 μm Millipore membrane ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in 500μL S-Trap lysis buffer (5% SDS, 50mM TEAB, pH 7.5) supplemented with cOmplete EDTA-free protease inhibitor (Sigma) and HALT phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Plant Biology 2021Quote: ... seeds were plated on solid growth media (GM) consisting of g/L Murashige and Skoog basal salt mixture (Sigma), 1% sucrose ...
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bioRxiv - Plant Biology 2020Quote: ... clv2 and fin mutants was conducted using half strength LANS containing 2.5 mM NaH2PO4 and 5.6 mM KNO3 (Hewitt 1966) solidified with 5g phytagel/L (Sigma, USA). In subsequent experiments that aimed to examine the tomato root morphology response under different N conditions ...
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bioRxiv - Microbiology 2020Quote: Approximately 20 µL of cells were air-dried either on agarose pads (1% (w/v) in PBS) or on slides coated with 0.1% (w/v) poly-l-lysine solution (Sigma) and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: Fresh solutions of L-NAME were prepared daily in accordance with previously published protocols (López-Juárez et al., 2017; L-NG-nitroarginine methyl ester, 100 mM in 13 PBS; Sigma-Aldrich) and injected to animals intraperitoneally (0.4 mg/kg ...
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bioRxiv - Microbiology 2021Quote: ... The resulting construct was transformed into protoplasts of each strain and mutants were selected for on osmotically stabilized minimal media (GMM plus 1.2M sorbitol) containing 100 μg/L pyrithiamine hydrobromide (Sigma). Reconstitution of the alaA gene was performed by PCR amplification of the alaA locus for each strain from ∼1.1kb upstream of the start codon to ∼700bp downstream of the stop codon using primers containing PacI and AscI digestion sites ...
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bioRxiv - Systems Biology 2020Quote: ... the MPAO1 cryoculture was streaked out on 20% BHI solid medium (7.4 g in 1 L water) containing 1.5 % agar (both Sigma, Switzerland). Shaken 20% BHI fluid cultures were inoculated from a single colony and grown at 30 °C until mid-exponential phase (OD600 = 0.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with selection medium (SM, 8 mM L-glutamine and phenol red, with 0.5 mg/mL G-418, Sigma). Cell pools were maintained at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Neurospheres were dissociated to single-cell and were seeded on poly-L-ornithine/laminin (Sigma, Saint Louis, Missouri, USA) coated plates for 24h in neurosphere medium ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown in a 1-L Braun Biostat fermenter supplemented with 15NH4Cl and D-[13C] glucose (Sigma-Aldrich) as the sole source of nitrogen and carbon for the bacterial cells ...
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bioRxiv - Biophysics 2020Quote: ... A single colony isolate was grown overnight and then sub-cultured in 50 ml tryptone media (0.015g/l; Sigma) till mid-log phase (OD 600 at 200 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell suspension was resuspended in BrainPhys complete media and 1.13×105 cells/cm2 were seeded in plates coated with 15μg/mL Poly-L-Ornithine (Sigma #P4957) and 0.5μg/cm2 laminin (Gibco #23017-015) ...
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bioRxiv - Cancer Biology 2020Quote: Cofluent astrocytes were irradiated with 0 Gy or 10 Gy followed by 10 days of culture on 0.2% gelatin in astrocyte medium supplemented with 50 µg/ml L-ascorbic acid (Sigma). After 10 days ...
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bioRxiv - Microbiology 2022Quote: ... UK) supplemented with 0.05 mg/mL mupirocin and 0.05 mg/mL of L-cysteine (both from Sigma-Aldrich, UK) within four hours of collection ...