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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... incubated for 1 hr with a 1:10,000 dilution alkaline phosphatase conjugated anti-mouse secondary antibody (Sigma), prior to a final 5 x TBST washes and incubation with BCIP/NBT substrate ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
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bioRxiv - Microbiology 2024Quote: ... for 1 h and then incubated with fluorescein (FITC)-conjugated secondary anti-mouse antibody (1:250, Sigma) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: PLA was performed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, DUO92101) protocol with some modifications ...
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bioRxiv - Immunology 2024Quote: ... BMMCs were sensitized overnight with 0.5 µg/mL mouse monoclonal anti-DNP IgE (SPE-7, Sigma-Aldrich). After sensitization ...
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bioRxiv - Immunology 2024Quote: ... This was followed by detection with a goat polyclonal anti-mouse IgG HRP-conjugate (1/1000; Millipore). For all ELISAs ...
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bioRxiv - Physiology 2024Quote: ... CLDN5 was identified with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma-Aldrich, MO, USA). Western blot signal was detected with a GeneGnome XRQ fluorescent detector (Syngene ...
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bioRxiv - Cell Biology 2024Quote: ... The lung fibroblast was maintained in Mouse Embryonic Fibroblast (MEF) medium on a gelatin (Millipore-Sigma, ES006B)-coated well of 6-well plates (0.1 million cells per well) ...
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bioRxiv - Cell Biology 2024Quote: ... The lung fibroblast was maintained in Mouse Embryonic Fibroblast (MEF) medium on a gelatin (Millipore-Sigma, ES006B)-coated well of 6-well plates (0.1 million cells per well) ...
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bioRxiv - Neuroscience 2024Quote: ... and a mouse monoclonal antibody against VSM α-Smooth Muscle actin – Cy3 (5mg/ml, Cat# C6198, Sigma). Amyloid was stained with methoxy-X04 overnight (1:500 from in 5mM in distilled H2O ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse α-smooth-muscle-actin (α-SMA, 1:1000, Sigma, St. Louis, MO; catalog no. A5691).
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bioRxiv - Neuroscience 2024Quote: ... corresponding secondary antibodies (anti-rabbit or anti-mouse HRP-conjugated, #AP307 and #AP130P respectively; Sigma-Aldrich, US) were added for 1 h at a dilution of 1/5000 ...
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bioRxiv - Cell Biology 2024Quote: ... or with mouse monoclonal anti-α-tubulin antibody (1:1000; T6199; Sigma Aldrich; Merck KGaA, Darmstadt, Germany) in blocking solution ...
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bioRxiv - Developmental Biology 2024Quote: ... the zygotes/embryos were incubated overnight at 4°C with mouse anti-α-tubulin (1:300, Sigma), rabbit monoclonal anti-Myo19 antibody (1:300 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The cells were reacted with rabbit anti-HA antibody and mouse anti-FLAG antibody (#F3165, Sigma-Aldrich) diluted with antibody reaction buffer [PBS ...
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bioRxiv - Molecular Biology 2020Quote: ChIPs were performed on plasmid shuffled derivatives of KY3232 as described above with an antibody against the FLAG epitope (Sigma-Aldrich, 30 µL A2220) to immunoprecipitate FLAG-Rpb3 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7-NLS-eGFP-dCAS9KRAB were then transduced with lentiviral particles containing plasmids expressing individual sgRNAs and selected with Puromycin (Sigma-Aldrich cat no. P8833). For each gene of interest ...
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bioRxiv - Developmental Biology 2021Quote: ... where the Cre enzyme was inserted after excision from pSK-Cre1 plasmid (provided by Clifford Tabbin, Harvard Medical school, diluted in PBS containing 0.05% of Fast Green (Sigma-Aldrich, Saint-Quantin Fallavier, France) was injected into the lateral ventricle of embryos using a Hamilton needle (Dutsher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Molecular Biology 2022Quote: For Generation of stable AKT1 and KLF4 overexpressing cell lines the AKT1 (TRCN0000473539) and KLF4 (TRCN0000492053) overexpression plasmids were obtained from Sigma (Mission TRC3.0, Sigma Aldrich, USA). For lentivirus packing ...
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bioRxiv - Bioengineering 2024Quote: Bacterial expression plasmids carrying CODEs were transformed into homemade competent cells propagated from Rosetta™ 2(DE3)pLysS Singles™ Competent Cells (Millipore Sigma, Cat# 71401). Individual colonies were picked and inoculated in 50 mL Luria Broth (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... the IA2 geneblock within the Pet53dest plasmid was isolated and mixed with BL21DE3 competent cells and grown prior to IPTG (Millipore Sigma, Cat#367-93-1) and EtOH induction of gene expression ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant from this clarification step were incubated with mouse anti-FLAG antibody (Sigma, clone M2 1µg/ml) over night at 4ºC with gentle rocking ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were incubated with TBST-Blotto containing primary monoclonal mouse anti-FLAG M2 antibody (1:2,000 dilution, Sigma) or monoclonal mouse anti-GAPDH antibody (1:2,000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were probed overnight at 4°C with mouse anti-BRAF [clone 3C6] (Sigma-Aldrich, cat# WH0000673M1; RRID:AB_1840330) at 2.5 µg/ml dilution ...
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bioRxiv - Systems Biology 2020Quote: ... samples were analyzed for β-actin expression using a mouse anti-β-actin antibody (1:5,000, Sigma Aldrich) and a HRP-labeled goat anti-mouse antibody (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
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bioRxiv - Microbiology 2020Quote: ... Detection was via polyclonal goat secondary antibodies: an ATTO647N-conjugated anti-mouse antibody was purchased from Sigma Aldrich, while an AlexaFluor488 (AF488)-conjugated anti-rabbit antibody was purchased from Invitrogen.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore; MA, USA), 1:200 goat anti-DCX(Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with ultra-block solution (10% rat sera, 10% hamster sera, 10% mouse sera (Sigma), and 10 μg/ml of anti-CD16/32 (2.4G2 ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibodies were used: mouse anti-acetylated tubulin (clone 6-11b-1, Sigma-Aldrich, diluted 1:2000) and goat anti-mouse Alexafluor 488 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Detection was done with donkey anti-mouse IgG horseradish peroxidase at 1:5000 (1:5000, Millipore Cat#AP308P) and Luminata Crescendo Western HRP substrate (Millipore Cat#WBLUR0100) ...
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bioRxiv - Bioengineering 2022Quote: ... The following primary antibody were used in this study: β-tubulin III (mouse, Sigma Aldrich T8660, 1:1000), TH (rabbit ...
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bioRxiv - Neuroscience 2022Quote: ... Co-stainings were performed with a rabbit (1:400) or mouse anti-GFAP (Sigma Aldrich, Cat. SAB5600060-RM246) or a rat anti-Lamp1 (1:300-Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... and a mouse anti-acetylated α-tubulin antibody (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) to co-stain cell boundaries ...
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bioRxiv - Developmental Biology 2022Quote: ... and a mouse anti-acetylated α-tubulin antibody (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) to co-stain cell boundaries ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with HRP-linked secondary antibodies anti-mouse IgG (1:5000, A6782, Sigma-Aldrich, MO, USA) and anti-rabbit IgG (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons isolated from E16 mouse embryos were cultured on 24-well plates coated with fibronectin (Sigma-Aldrich). The cells were infected with lentivirus-E-SARE-Venus-AkaLuc-PEST on DIV5 ...
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bioRxiv - Molecular Biology 2021Quote: Proximity ligation assays were carried out with Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of FLAG-AGO2 was performed with Dynabeads Protein G (Life) and anti-FLAG M2 mouse (Sigma, F3165) for 3h at 4ºC with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with goat anti-mouse IgG conjugated to horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) diluted 1 ...
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bioRxiv - Molecular Biology 2021Quote: PLA was performed using DuoLink red detection kit and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Genomics 2019Quote: Mouse ES cells were routinely cultured on cell culture-treated dishes coated with 0.1% gelatin (Sigma #G9391-100G) using the following culture medium ...
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bioRxiv - Genomics 2019Quote: MIN6 mouse insulinoma beta cells50 were grown in Dulbecco’s modified Eagle’s Medium (Sigma-Aldrich, St. Louis, Missouri/USA) with 10% fetal bovine serum ...
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bioRxiv - Biophysics 2019Quote: ... Following primary antibodies were used: mouse monoclonal anti-pan sodium channel antibody (panNav; Sigma Aldrich, cat. no. S8809) and mouse monoclonal anti-ankyrin G antibody (Santa Cruz ...
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bioRxiv - Genetics 2019Quote: ... The bound anti-BrdU antibody was subsequently detected by anti-mouse IgG-FITC conjugate (Sigma, dilution 1:64) under a fluorescence microscope after mounting the ganglia in antifade (Sigma).
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bioRxiv - Cell Biology 2020Quote: The primary antibodies used (with their respective working dilutions) were: anti-FLAG (Sigma F1804, mouse, WB: 1/1000), anti-pan-acetyl lysine (AcK ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were used: Monoclonal mouse anti-FLAG M2-Peroxidase (HRP) (Sigma-Aldrich; A8592, dilution: 1:1,000). Monoclonal mouse anti-Actin clone C4 (Millipore ...