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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301, Goat α-mouse HRP 1:1000– Sigma Aldrich/GERPN4401), revealed using ECL reagent (BioRad ...
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bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
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bioRxiv - Microbiology 2024Quote: ... washed in water until the stain turned blue approximately 2 mins and then counter stained with 1% Eosin (Sigma-Aldrich, Cat. no. 17372-87-1) for 30 Sec ...
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bioRxiv - Neuroscience 2024Quote: ... Under anesthesia by intramuscular injection of 0.4 mL of a 1:1 mixture of ketamine (10 mg/mL, Daiichi-Sankyo Pharma Co., Tokyo, Japan) and xylazine (2 mg/mL, Sigma-Aldrich Co., St Louis, USA), the left hemisphere was dissected and immersed in ice-chilled Krebs solution composed of (in mM) ...
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bioRxiv - Genetics 2021Quote: ... 2 mM EDTA and 1% Triton X-100) with 20 mg/mL lysozyme from chicken egg (Sigma, L4919) using a tissue grinder (BMS BC-G10 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos older than 24hpf were transferred into E3 medium containing 0.003% 1-phenyl 2-thiourea (PTU, Sigma P7629) to inhibit pigment formation and aid imaging.
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... t Phospho-Myosin Light Chain 2 (Ser19) antibody (3671; Cell Signalling) 1:50 and Recoverin antibody (AB5585; Millipore) 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Sigma) (sigma) ...
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bioRxiv - Immunology 2022Quote: ... All wells were treated for 1 hour with 10 µM 2’,7’-dichlorofluorescin diacetate (DCF-DA, Sigma, D6883) in Ringer buffer (155 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min and blocked for at least 2 h with a blocking buffer containing 1% BSA (Sigma), 3% donkey serum (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Evolutionary Biology 2020Quote: Adult male frogs (n = 2) were anesthetized with 1% MS-222 (tricaine, Sigma-Aldrich, St. Louis, MO, USA) and sacrificed by decapitation ...
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bioRxiv - Cell Biology 2021Quote: ... topped up to 140 ug/ul for the final 2 h and then DNA was extracted with 20 ml Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Sigma), precipitated with ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2020Quote: ... HIV-GFP produced with p8.91 or p8.2 used in Figure 1 were DNase treated for 2 hours at 37°C (DNaseI, Sigma) prior to ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Neuroscience 2020Quote: ... A borosilicated glass micropipette was filled with NES and 2 mM Glutamate or 1 mM GABA (Sigma Aldrich), and moved via a micromanipulator MP-225 (Sutter Instruments ...
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich catalogue no D9542, 1:600 dilution). Figure 4 shows images of seedlings from 1 of 6 experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Filtered cells were immediately seeded in 96 well plates coated with 1% Poly (2-hydroxyethyl methacrylate; Sigma Aldrich) at a density of 200 or 50 cells per well ...
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bioRxiv - Cell Biology 2021Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW: 114,17; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were stained by addition of 1 µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) containing FluoromountG (Southern Biotech) ...
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bioRxiv - Cell Biology 2020Quote: ... All embryos were maintained in Ringer’s solution at 28.5°C and treated with 0.003% 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) from 8 hpf.
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543 ...
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bioRxiv - Biochemistry 2022Quote: ... Fatty acids were extracted using a chloroform-methanol mix (2:1; Thermo-Fisher) in 0.01% butylated hydroxytoluene (Sigma) prior to centrifugation at 200 g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: A375 cells in 100 mm dishes were challenged by 2 mM of octanoate-1-13C (Sigma Aldrich #296457) for 6 or 20 hrs ...
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bioRxiv - Cancer Biology 2022Quote: ... the cover slides were treated with 1 mg/ml N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sigma) and exposed to ultra-violet (UV ...
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bioRxiv - Cell Biology 2022Quote: Evaporated lipids were resuspended in 10µL 2:1 chloroform/methanol and spotted on dehydrated silica plates (EMD Millipore; dehydrated by baking for 30 minutes at 100°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immobilized larvae were then immersed in MS-222 (0.02%) and 1-phenyl-2-thiourea (PTU, 0.003%, Sigma-Aldrich) containing E3 during imaging ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with secondary antibodies for 2 h: Alexa Fluor 488 goat anti-mouse IgG (1:400, Millipore), or Fluor 594 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... The quenched samples were concentrated to 60 μl by centrifugation at 20,000xg for 2 min (Sigma 1-16K). Due to the linear nature of DNA ...
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bioRxiv - Neuroscience 2022Quote: ... samples were stained with the fluorescent nucleic acid marker 4,6-diamino-2-fenilindol (DAPI 1:3000; Sigma-Aldrich), and mounted with a Fluorescent Mounting Medium (DAKO ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000 dilution with milliQ water, Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 2:1 ratio of beads to reaction volume with an additional 1.8x supplementation of isopropanol (Sigma).
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bioRxiv - Physiology 2020Quote: ... Nuclei were counter-stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 1/5000) (Sigma-Aldrich, Dorset, UK). Imaging was performed on a Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Genetics 2021Quote: ... the resin with the immobilized MBP-Rif2 variants was first washed 4 times with 1 ml wash buffer containing 1M NaCl (50 mM Tris-HCl pH 7.5, 1 M NaCl, 0.2% NP40, 2 mM EDTA, Sigma protease inhibitory cocktail P8340 1:1000) ...
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bioRxiv - Cancer Biology 2021Quote: ... universal MISSION® siRNA Universal Negative Control #1 (cat. SIC001) and #2 (cat. SIC002) were from Millipore Sigma. We used the DharmaFECT 4 Transfection Reagent (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... pH 7) supplemented with 0.02 mg/ml of 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich, St. Louis, MO) to prevent pigmentation ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Physiology 2020Quote: ... following which they were incubated with anti-MAP-2 antibody (AB5543, 1:500, Merck Millipore, Burlington, MA, USA) in blocking buffer overnight at 4℃ ...
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bioRxiv - Biochemistry 2021Quote: ... resin was incubated with 2 bed vol of PBS containing 10 μL of 1 U/μL thrombin (Sigma) for 2 h at 25°C with occasional agitation ...
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bioRxiv - Developmental Biology 2021Quote: ... endogenous pigmentation was inhibited from 24 hpf by addition of 0.003% 1-phenyl-2-thiourea (PTU, Sigma Aldrich) in E3 medium.
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bioRxiv - Biochemistry 2021Quote: ... The cellular suspension was distributed in portions of 0.8 millilitres to reaction tubes with 2 millilitre volume to which 1.4 grams of glass beads (0.75 - 1 millimetre, Sigma) were added ...
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bioRxiv - Molecular Biology 2021Quote: ... see Table 1 for complete plasmid list) was induced by treating cells with 2 µg/mL doxycycline (Sigma) for at least 72 hours ...
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bioRxiv - Biophysics 2021Quote: ... then washed and resuspended in the same volume of YEPD (2% glucose) medium containing 1 mM auxin (Sigma). These cells were grown in the presence of auxin for another 20 minutes at 30 °C to deplete Rbg1 completely.
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bioRxiv - Microbiology 2020Quote: ... The SDS-PAGE gel was stained for 2 h using staining solution [Brilliant Blue (1 g; Sigma-Aldrich), methanol (50% v/v ...
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bioRxiv - Immunology 2020Quote: Cell suspensions were stained for viability with either 1:3000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) or 1:1000 Zombie Aqua (Biolegend ...